Skip to main content
Addgene
Showing: 201 - 250 of 4188 results
  1. PA1421_WT_pET21a

    Plasmid
    #29792
    Depositor
    Insert
    Guanidinobutyrase (gbuA P. aeruginosa)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 4, 2012
    Availability
    Academic Institutions and Nonprofits only
  2. PA0288_WT_pET21a

    Plasmid
    #29793
    Depositor
    Insert
    3-guanidinopropionase (gpuA P. aeruginosa)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 4, 2012
    Availability
    Academic Institutions and Nonprofits only
  3. PA1421_M161Y_pET21a

    Plasmid
    #29791
    Depositor
    Insert
    Guanidinobutyrase (gbuA P. aeruginosa)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    M161Y
    Promoter
    Available since
    July 7, 2011
    Availability
    Academic Institutions and Nonprofits only
  4. PA0288_Y157M_pET21a

    Plasmid
    #29794
    Depositor
    Insert
    3-guanidinopropionase (gpuA P. aeruginosa)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Y157M
    Promoter
    Available since
    July 7, 2011
    Availability
    Academic Institutions and Nonprofits only
  5. pET28aHisAviCD72aCTLD

    Plasmid
    #129067
    Purpose
    generation of biotinylated mouse CD72a CTLD protein using bacteria
    Depositor
    Insert
    CD72a (Cd72 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
  6. pET28aHisAviCD72cCTLD

    Plasmid
    #129068
    Purpose
    generation of biotinylated mouse CD72c CTLD protein using bacteria
    Depositor
    Insert
    CD72c (Cd72 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
  7. pET28aHisAvihCD72CTLD

    Plasmid
    #129069
    Purpose
    generation of biotinylated human CD72 CTLD protein using bacteria
    Depositor
    Insert
    CD72 (CD72 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
  8. pET21b(+)-Is-PETase

    Plasmid
    #112202
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12
    Insert
    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    Promoter
    N/A
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pET28a-LIC

    Plasmid
    #26094
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Type
    Empty backbone
    Use
    Tags
    6xHis and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
  10. pET29a-YS14

    Plasmid
    #66890
    Purpose
    Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)
    Depositor
    Inserts
    His6-Thrombin-Xenopus laevis histones H2A
    Xenopus laevis histones H2B
    Xenopus laevis histones H3
    Xenopus laevis histones H4-Thrombin
    Use
    Tags
    H2A, H2B, H3, H4, His6 tag, and Thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
  11. eSrtA(2A-9) pET29b

    Plasmid
    #75145
    Purpose
    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity
    Depositor
    Insert
    reprogrammed sortase A variant
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    S102C A104H E105D K138P K152I N160K K162H T164N K…
    Promoter
    T7
    Available since
    July 28, 2016
    Availability
    Academic Institutions and Nonprofits only
  12. eSrtA(4S-9) pET29b

    Plasmid
    #75146
    Purpose
    reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity
    Depositor
    Insert
    reprogrammed sortase A variant
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    N98D S102C A104V A118T F122A K134R F144L I182V E1…
    Promoter
    T7
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
  13. pET28a hMDH2

    Plasmid
    #204230
    Purpose
    Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).
    Insert
    hMDH2 (MDH2 Human)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include transit peptide and includes a M…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  14. pET28a-HsSENP2

    Plasmid
    #71466
    Purpose
    Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Insert
    SUMO1/sentrin specific peptidase 2 (SENP2 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  15. pET29b-rhMOG

    Plasmid
    #225484
    Purpose
    Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli
    Depositor
    Insert
    Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-29 (signal peptide) and 150…
    Promoter
    T7
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  16. pET28-MKH8SUMO

    Plasmid
    #79526
    Purpose
    Bacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    8xHis-Thrombin-SUMO-TEV and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 201 - 250 of 4188 results