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Plasmid#180660PurposeEncodes pLacFBA1 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systemsDepositorInsertpLacFBA1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT005
Plasmid#180658PurposeEncodes pLacFEC as a Type 2 part to be used in the Dueber YTK and Ellis MYT systemsDepositorInsertpLacFEC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT004
Plasmid#180657PurposeEncodes pLacC as a Type 2 part to be used in the Dueber YTK and Ellis MYT systemsDepositorInsertpLacC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT003
Plasmid#180656PurposeEncodes pLacE as a Type 2 part to be used in the Dueber YTK and Ellis MYT systemsDepositorInsertpLacE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT002
Plasmid#180655PurposeEncodes pLacD as a Type 2 part to be used in the Dueber YTK and Ellis MYT systemsDepositorInsertpLacD
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)
Plasmid#225752PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQP-T2A
Plasmid#102583PurposeLight-controlled activation of expressionDepositorInsertBphP1-VP16-T2A-NLS-GAL4-QPAS1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-nCas9n
Plasmid#46757DepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionBacterialMutationPromoterT3Available sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-FUS-G156E
Plasmid#181716PurposeGalactose inducible expression of FUS-G156EDepositorInsertFUS (FUS Human)
UseTagsExpressionYeastMutationG156EPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Alpha-synuclein
Plasmid#181715PurposeGalactose inducible expression of Alpha-synucleinDepositorInsertAlpha-synuclein (SNCA Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector
Plasmid#194715PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sitesDepositorArticleInsertAsCpf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
act5c-LbCas12a
Plasmid#140620PurposeUbiquitous expression of LbCas12a in DrosophilaDepositorInsertLbCas12a
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500)
Plasmid#225753PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Zeocin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Alpha-synuclein-E46K
Plasmid#181723PurposeGalactose inducible expression of Alpha-synuclein-E46KDepositorInsertAlpha-synuclein (SNCA Human)
UseTagsExpressionYeastMutationE46KPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Alpha-synuclein-H50Q
Plasmid#181724PurposeGalactose inducible expression of Alpha-synuclein-H50QDepositorInsertAlpha-synuclein (SNCA Human)
UseTagsExpressionYeastMutationH50QPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHG6
Plasmid#188254PurposeIntegrating plasmid contains the BT1 int/attP locus and the erythromycin-resistance gene for selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoteract1p/actII-orf4Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRec ΔLacI
Plasmid#104575PurposeExpresses Cas1, Cas2 under PLTetO-1 promoter, and TetR constitutively.DepositorInsertPTet-cas12, tetR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHG7
Plasmid#188255PurposeIntegrating plasmid contains the C31 int/attP locus and the hygromycin-resistance gene for selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoteract1p/actII-orf4Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Alpha-synuclein-A53T
Plasmid#181722PurposeGalactose inducible expression of Alpha-synuclein-A53TDepositorInsertAlpha-synuclein (SNCA Human)
UseTagsExpressionYeastMutationA53TPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFD8
Plasmid#140619PurposeExpression of one of several LbCas12a crRNAs in DrosophilaDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterDrosophila U6:3Available sinceOct. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRec
Plasmid#104572PurposeExpresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI constitutively.DepositorInsertPTet-cas12, tetR, lacI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ACME
Plasmid#186717PurposeACME dual-deaminase CRISPR base editorDepositorInsertACME
UseCRISPRTagsNucleoplasmin NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-FUS
Plasmid#181711PurposeGalactose inducible expression of FUSDepositorInsertFUS (FUS Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7tyrgRNA
Plasmid#46761DepositorInserttyr gRNA (tyr Zebrafish)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHG5
Plasmid#188253PurposeIntegrating plasmid contains the SV1 int/attP locus and the kanamycin-resistance gene (aphII) for selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoteract1p/actII-orf4Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-hnRNPA1
Plasmid#181710PurposeGalactose inducible expression of hnRNPA1DepositorInserthnRNPA1 (HNRNPA1 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR PKAKTRn-Clover
Plasmid#177199PurposeGateway entry vector encoding PKAKTRnew-CloverDepositorInsertPKAKTRnew-mClover
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAST-LbCas12a
Plasmid#140621PurposeGal4 dependent expression of LbCas12a in DrosophilaDepositorInsertLbCas12a
UseCRISPRTagsExpressionInsectMutationPromoterUASTAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-sypHTomato
Plasmid#164613PurposeExpress the pHTomato inserted in the synaptophysion in mammalian cellsDepositorInsertA reporter of vesicular turnover sypHTomato
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-TDP-43
Plasmid#181709PurposeGalactose inducible expression of TDP-43DepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV:AsCpf1-2A-GFP-U6-AAVS1-sg
Plasmid#194716PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hAAVS1 locusDepositorArticleInsertAsCpf1 (AAVS1 Acidaminococcus sp.)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGRNA2e
Plasmid#169782PurposeInsert one gRNADepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGRNA1
Plasmid#169778PurposeInsert one gRNADepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1(D908A)-2A-GFP-U6-AAVS1-sgRNA
Plasmid#194727PurposeCAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector, with guide RNA array targeting hAAVS1.DepositorArticleInsertAsCpf1(D908A) (AAVS1 Acidaminococcus sp.)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA
Plasmid#194723Purposebased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1DepositorArticleInsertAsCpf1 (AAVS1 Acidaminococcus sp.)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti EPICode screen backbone
Plasmid#177198Purposelentiviral vector backbone for both barcodes and KTRDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJSH-B14
Plasmid#20145DepositorInsertArtificial sequence
UseCre/LoxTagsExpressionMutationn/aPromoterAvailable sinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGRNA3e
Plasmid#169783PurposeInsert one gRNADepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGRNA5e
Plasmid#169786PurposeInsert one gRNADepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGRNA4e
Plasmid#169784PurposeInsert one gRNADepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGRNA4
Plasmid#169781PurposeInsert one gRNADepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGRNA3
Plasmid#169780PurposeInsert one gRNADepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGRNA2
Plasmid#169779PurposeInsert one gRNADepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-SoxN-A22-EGFP
Plasmid#181731PurposeGalactose inducible expression of SoxN-A22-EGFPDepositorInsertSoxN-A22-EGFP (SoxN Fly)
UseTags3xFLAG-EGFPExpressionYeastMutationextended repeat of 22 Ala residues replacing nati…PromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-hnRNPA2B1
Plasmid#181714PurposeGalactose inducible expression of hnRNPA2B1DepositorInserthnRNPA2B1 (HNRNPA2B1 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVP293
Plasmid#222062PurposeGreenGate CD bridge module containing filler sequence, backbone domesticated of Esp3I sites.DepositorInsertFiller
UseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationBackbone Esp3i sites have been domesticatedPromoterAvailable sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP294
Plasmid#222063PurposeGreenGate DE bridge module containing filler sequence, backbone domesticated of Esp3I sites.DepositorInsertFiller
UseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationBackbone Esp3i sites have been domesticatedPromoterAvailable sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP096
Plasmid#222043PurposepGGPK-AG2 where the ccdb/CmR has been swappe for mRFP1e; Esp3I domesticated backbone.DepositorInsertmRFP1e
UseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVP076
Plasmid#222028PurposeEmpty spectinomycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.DepositorTypeEmpty backboneUseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationAll Esp3i sites domesticatedPromoterAvailable sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP077
Plasmid#222029PurposeEmpty Kanamycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.DepositorTypeEmpty backboneUseSynthetic Biology; Greengate compatible cloning v…TagsExpressionMutationAll Esp3i sites domesticatedPromoterAvailable sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits