We narrowed to 826 results for: tg;

1. Halo-WIPI2B K128E

   Plasmid

   #175031

   Purpose

   Expresses WIPI2B (K128E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

   Depositor

   Andrea Stavoe

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2B (K128E) (WIPI2 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Lysine 128 to Glutamate (AAG to GAG)

   Promoter

   CMV

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-WIPI2dR108E-cs-TEV-STREP

   Plasmid

   #171835

   Purpose

   Mammalian expression of human WIPI2d R108E with C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   R108E

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-WIPI2dR125E-cs-TEV-STREP

   Plasmid

   #171836

   Purpose

   Mammalian expression of human WIPI2d R125E with C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   R125E

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-WIPI2dK128E-cs-TEV-STREP

   Plasmid

   #171837

   Purpose

   Mammalian expression of human WIPI2d K128E with C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   K128E

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-mcherry-WIPI2dR108E-cs-TEV-STREP

   Plasmid

   #171842

   Purpose

   Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Tags

   Strep and mCherry

   Expression

   Mammalian

   Mutation

   R108E

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-WIPI2dH85E-cs-TEV-STREP

   Plasmid

   #171831

   Purpose

   Mammalian expression of human WIPI2d H85E with C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   H85E

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-WIPI2dK88E-cs-TEV-STREP

   Plasmid

   #171832

   Purpose

   Mammalian expression of human WIPI2d K88E with C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   K88E

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-WIPI2dI92E-cs-TEV-STREP

   Plasmid

   #171833

   Purpose

   Mammalian expression of human WIPI2d I92E with C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   I92E

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-WIPI2dC93E-cs-TEV-STREP

   Plasmid

   #171834

   Purpose

   Mammalian expression of human WIPI2d C93E with C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   C93E

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPEE42

    Plasmid

    #139421

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    Citrine
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPEE45

    Plasmid

    #139424

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mTurquoise1
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPEE44

    Plasmid

    #139423

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTFP1 ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mTFP1
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPEE43

    Plasmid

    #139422

    Purpose

    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mMaroon1
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGWKS17

    Plasmid

    #139420

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised mCherry marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mCherry
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA-dabAB2

    Plasmid

    #133012

    Purpose

    pFA carrying the badabA2 (locus_tag: BAS2958) and badabB2 genes (locus_tag: BAS2959)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Expression

    Bacterial

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFE-dabAB2 C539A

    Plasmid

    #133008

    Purpose

    pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a C539A mutation and a dabB2 gene  (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Expression

    Bacterial

    Mutation

    C539A

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFE-dabAB2 C351A

    Plasmid

    #133005

    Purpose

    pFE carrying a dabA2 gene with a C351A mutation and a dabB2 gene (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Expression

    Bacterial

    Mutation

    C351A

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFE-dabAB2 H524A

    Plasmid

    #133007

    Purpose

    pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a H524A mutation and a dabB2 gene (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Expression

    Bacterial

    Mutation

    H524A

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFE-dabAB2 D353A

    Plasmid

    #133006

    Purpose

    pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a D353A mutation and a dabB2 gene  (Uniprot: D0KWS8)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Expression

    Bacterial

    Mutation

    D353A

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p073 pCS xGATA4tr + xTRPS1(C119)

    Plasmid

    #11044

    Depositor

    Ramesh Shivdasani

    Article

    Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

    Insert

    GATA4tr + TRPS1(C119) (trps1, gata4-b Frog)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Mutation

    Fusion construct of XGATA4 (amino acids 1-274) wi…

    Available Since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVX.TRE3G.AdProt

    Plasmid

    #131687

    Purpose

    A lentiviral vector for tTA-regulated expression of the adenovirus protease using the tetracycline transactivator transcription factor.

    Depositor

    Matthew D. Shoulders

    Article

    Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

    Insert

    Adenovirus Protease
    

    Use

    Lentiviral

    Promoter

    TRE3GV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p-AAV-sh[control]

    Plasmid

    #75438

    Purpose

    control shRNA vector

    Depositor

    Edward Burton

    Article

    Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

    Insert

    GFP
    

    Use

    AAV and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SynTagMA_post

    Plasmid

    #119736

    Purpose

    Synaptic Tag for Mapping Activity

    Depositor

    Thomas Oertner

    Article

    Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

    Insert

    SynTagMA
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    hSyn-1

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDH_CMV_TFAM_mKate2

    Plasmid

    #167331

    Purpose

    Expresses mKate2 fused to full length TFAM in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SynTagMA_pre

    Plasmid

    #119738

    Purpose

    Synaptic Tag for Mapping Activity

    Depositor

    Thomas Oertner

    Article

    Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

    Insert

    SynTagMA_pre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hsyn1

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28-His6-Ubc9

    Plasmid

    #133909

    Purpose

    Bacterial expression of His6-Ubc9

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV CaMKIIa iChloC 2A tDimer

    Plasmid

    #66709

    Purpose

    Chloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent protein

    Depositor

    Thomas Oertner

    Article

    Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E83Q,E90R,E101S,D156N,T159C

    Promoter

    CaMKIIa

    Available Since

    Oct. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET41a iGluu

    Plasmid

    #119834

    Purpose

    Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluu
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    S72T

    Promoter

    T7

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)

    Plasmid

    #164193

    Purpose

    Freeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)

    Depositor

    Thomas Oertner

    Article

    Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

    Inserts

    CamPARI2
    Human α Tubulin
    

    Use

    AAV

    Tags

    6 His tag and NES

    Expression

    Mammalian

    Mutation

    F391W-L398V

    Promoter

    Syn

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Wild-type CoV-2 ORF8

    Plasmid

    #159933

    Purpose

    Protein overexpression in E.coli

    Depositor

    James Hurley

    Article

    Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

    Insert

    CoV-2 ORF8 18-121 (ORF8 CoV-2)
    

    Tags

    6x Histidine, TEV cleavable

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCI syn iGluu

    Plasmid

    #106122

    Purpose

    Fast indicator for synaptic glutamate imaging (synapsin promoter)

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR S72T variant
    

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Ser 72 to Thr

    Promoter

    synapsin

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDNA3.0_mitoLAMA-F98 IRES ShadowG-mScarlet

    Plasmid

    #130708

    Purpose

    Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-LAMA-F98 IRES ShadowG-mScarlet
    

    Tags

    NTOM20 and SNAP-Tag

    Expression

    Mammalian

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDNA3.0_LynLAMA-G97 IRES EGFP

    Plasmid

    #130711

    Purpose

    Plasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    Lyn11-SNAP-Tag-LAMA-G97 IRES EGFP
    

    Tags

    Lyn11 and SNAP-Tag

    Expression

    Mammalian

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCDNA3.0_LynLAMA-F98 IRES EGFP

    Plasmid

    #130710

    Purpose

    Plasmid encoding outer inner membrane localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    Lyn11-SNAP-Tag-LAMA-F98 IRES EGFP
    

    Tags

    Lyn11 and SNAP-Tag

    Expression

    Mammalian

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Empty Target Control

    Plasmid

    #127667

    Purpose

    Encodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET41a iGluSnFR

    Plasmid

    #119828

    Purpose

    Production of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDNA3.0_mitoLAMA-F98 IRES EGFP

    Plasmid

    #130706

    Purpose

    Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-LAMA-F98 IRES EGFP
    

    Tags

    NTOM20 and SNAP-Tag

    Expression

    Mammalian

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET41a iGluh

    Plasmid

    #119830

    Purpose

    Production of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluh
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E25A

    Promoter

    T7

    Available Since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV Syn ChR E90R-T159C 2A tDimer

    Plasmid

    #52495

    Purpose

    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E90R, T159C

    Promoter

    human synapsin-1 and ribosomal skip sequence

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet

    Plasmid

    #130709

    Purpose

    Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet
    

    Tags

    NTOM20 and SNAP-Tag

    Expression

    Mammalian

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET41a iGluf

    Plasmid

    #119833

    Purpose

    Production of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluf
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E25D

    Promoter

    T7

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCI syn iGluf

    Plasmid

    #106121

    Purpose

    Fast indicator for synaptic glutamate imaging (synapsin promoter)

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR E25D variant
    

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Glu 25 to Asp

    Promoter

    synapsin

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. V32M,L84M CoV-2 ORF8

    Plasmid

    #159934

    Purpose

    Protein overexpression in E.coli

    Depositor

    James Hurley

    Article

    Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

    Insert

    CoV-2 ORF8 18-121 (ORF8 CoV-2)
    

    Tags

    6x Histidine, TEV cleavable

    Expression

    Bacterial

    Mutation

    V32M, L84M

    Promoter

    T7

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCAG-WIPI2d10-364delta263-295-TEV-STREP

    Plasmid

    #171830

    Purpose

    Mammalian expression of human WIPI2d 10-364 delta263-295 with C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d (WIPI2 Human)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    10-364delta263-295

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pFE-vcDAB

    Plasmid

    #133004

    Purpose

    pFE carrying the vcdabA2 ( locus_tag: CSW01_RS07960) and vcdabB2 genes (locus_tag: CSW01_RS07955)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    vcdabB
    vcdabA
    

    Expression

    Bacterial

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pET30b GluBP600n

    Plasmid

    #119835

    Purpose

    Production of recombinant GluBP600n high affinity protein in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    GluBP600n
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pET41a iGlul

    Plasmid

    #119832

    Purpose

    Production of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGlul
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    T92A

    Promoter

    T7

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCDNA3.0_mitoLAMA-G97 IRES EGFP

    Plasmid

    #130707

    Purpose

    Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-LAMA-G97 IRES EGFP
    

    Tags

    NTOM20 and SNAP-Tag

    Expression

    Mammalian

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pAAV CaMKIIa ChR2 E90R D156N T159C 2A tDimer

    Plasmid

    #52878

    Purpose

    Chloride-conducting Channelrhodopsin with slow kinetics, neuron-specific promoter, plus red fluorescent protein, excitatory neurons

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E90R, D156N, T159C

    Promoter

    CaMKIIa

    Available Since

    July 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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50. pGG-PIP62

    Plasmid

    #196800

    Purpose

    Contains EDS1 promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1 Mustard Weed)
    

    Use

    Greengate (gg) entry vector

    Mutation

    151 G->C and 572 A->T

    Available Since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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