We narrowed to 106 results for: 62988

1. pSpCas9(BB)-2A-Puro (PX459) V2.0

   Plasmid

   #62988

   Purpose

   Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pX461-ABE8e-nSpCas9-Intergenic sgRNA

   Plasmid

   #237463

   Purpose

   All-in-one base editor expressing adenine base editor (ABE8e-nSpCas9) and gRNA control targeting Intergenic site

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Kumari et al Nat Commun 17, 1218 (2026)

   Insert

   Intergenic sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chicken beta-actin promoter & U6

   Available Since

   Jan. 12, 2026

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX461-evoCDA1-nSpCas9-Intergenic sgRNA

   Plasmid

   #237464

   Purpose

   All-in-one base editor expressing cytosine base editor (evoCDA1-nSpCas9) and sgRNA control targeting Intergenic site

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Kumari et al Nat Commun 17, 1218 (2026)

   Insert

   Intergenic sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chicken beta-actin promoter & U6

   Available Since

   Jan. 12, 2026

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX462-ABE8e-nSaCas9-Intergenic sgRNA

   Plasmid

   #237465

   Purpose

   All-in-one base editor expressing adenine base editor (ABE8e-nSaCas9) and sgRNA control targeting Intergenic site

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Kumari et al Nat Commun 17, 1218 (2026)

   Insert

   Intergenic sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chicken beta-actin promoter & U6

   Available Since

   Jan. 12, 2026

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX461-ABE8e-nSpCas9-NRF1-ss-sgRNA

   Plasmid

   #237466

   Purpose

   All-in-one adenine base editor (ABE8e-nSpCas9) with gRNA targeting NRF1-Ex7 splice site

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Kumari et al Nat Commun 17, 1218 (2026)

   Insert

   gRNA targeting NRF1-Ex7 splice site
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chicken beta-actin promoter & U6

   Available Since

   Jan. 12, 2026

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX461-evoCDA1-nSpCas9-NRF1-ss-sgRNA

   Plasmid

   #237467

   Purpose

   All-in-one cytosine base editor (evoCDA1-nSpCas9) with sgRNA targeting NRF1-Ex7 splice site

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Kumari et al Nat Commun 17, 1218 (2026)

   Insert

   sgRNA targeting NRF1-Ex7 splice site
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chicken beta-actin promoter & U6

   Available Since

   Jan. 12, 2026

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX462-ABE8e-nSaCas9-NRF1-ss-sgRNA

   Plasmid

   #237468

   Purpose

   All-in-one adenine base editor (ABE8e-nSaCas9) with gRNA targeting NRF1-Ex7 splice site

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Kumari et al Nat Commun 17, 1218 (2026)

   Insert

   gRNA targeting NRF1-Ex7 splice site
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chicken beta-actin promoter & U6

   Available Since

   Jan. 12, 2026

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PX459_NRF2-exon5-1

   Plasmid

   #177781

   Purpose

   sgRNA for CRISPR/Cas9-mediated deletion of human NRF2

   Depositor

   Michael Ristow

   Article

   Thomas et al Nat Commun. 2023 Dec 8;14(1):8142. doi:

   Insert

   NRF2 (NFE2L2 Human)
   

   Use

   CRISPR

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PX459_NRF2-exon5-2

   Plasmid

   #177782

   Purpose

   sgRNA for CRISPR/Cas9-mediated deletion of human NRF2

   Depositor

   Michael Ristow

   Article

   Thomas et al Nat Commun. 2023 Dec 8;14(1):8142. doi:

   Insert

   NRF2 (NFE2L2 Human)
   

   Use

   CRISPR

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

   Plasmid

   #221552

   Purpose

   Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

   Insert

   gRNA targeting human Rab7A (RAB7A Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. INS-3'gRNA

    Plasmid

    #210468

    Purpose

    gRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    INS (INS Human)
    

    Use

    CRISPR

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PX459_GRHL1-exon5

    Plasmid

    #177763

    Purpose

    sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    CRISPR

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PX459_GRHL1-exon8

    Plasmid

    #177764

    Purpose

    sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    CRISPR

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEN243-EN1953

    Plasmid

    #224546

    Purpose

    Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl

    Depositor

    Elphege Nora

    Article

    Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

    Insert

    Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl (Nipbl Mouse)
    

    Expression

    Mammalian

    Available Since

    Dec. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PX459v3

    Plasmid

    #178799

    Purpose

    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PX459v2-ILK-gRNA 1-exon 1

    Plasmid

    #163320

    Purpose

    SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ILK gRNA 1_Exon1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

16. PX459v2-ILK-gRNA 2-exon 8

    Plasmid

    #163321

    Purpose

    SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ILK gRNA 2_Exon8
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6F

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

17. pX461-ABE8e-nSpCas9

    Plasmid

    #237460

    Purpose

    Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Kumari et al Nat Commun 17, 1218 (2026)

    Insert

    3xFlag_ABE8e_SpCas9(D10A)_6xNLS
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    chicken beta-actin promoter & U6

    Available Since

    Jan. 12, 2026

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX461-evoCDA1-nSpCas9

    Plasmid

    #237461

    Purpose

    Transfection-based all-in-one base editor plasmid that expresses both cytosine base editor (evoCDA1-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Kumari et al Nat Commun 17, 1218 (2026)

    Insert

    3xFlag_evoCDA1_ssDBD_nickase SpCas9 _6xUGI
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    chicken beta-actin promoter & U6

    Available Since

    Jan. 12, 2026

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX462-ABE8e-nSaCas9

    Plasmid

    #237462

    Purpose

    Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSaCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Kumari et al Nat Commun 17, 1218 (2026)

    Insert

    3xFlag_ABE8e_SaCas9 -6xNLS
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    chicken beta-actin promoter & U6

    Available Since

    Jan. 12, 2026

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEN243-KH217

    Plasmid

    #224550

    Purpose

    Cas9-2A-puro and sgRNA targeting the STOP codon mouse Car2

    Depositor

    Elphege Nora

    Article

    Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

    Insert

    Cas9 and sgRNA targeting the STOP codon of mouse CAR2 (Car2 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge