We narrowed to 220 results for: lifeact

1. mGeos-M-Lifeact-7

   Plasmid

   #54816

   Purpose

   Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mGeos-M-Lifeact

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

2. mTopaz-Lifeact-7

   Plasmid

   #54661

   Purpose

   Localization: Actin, Excitation: 514, Emission: 527

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mTopaz-Lifeact

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. EGFP-lifeactin

   Plasmid

   #229606

   Purpose

   Expresses EGFP-lifectin fusion in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mKOet-Lifeact-7

   Plasmid

   #54633

   Purpose

   Localization: Actin, Excitation: 548, Emission: 561

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mKOet

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA5/FRT/TO_Lifeact_HaloTag9

   Plasmid

   #175545

   Purpose

   Expression of HaloTag9 at the actin filaments of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lifeact-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-Lifeact-tdTomato

   Plasmid

   #64048

   Purpose

   Fluorescent reporter for F-actin labeling in living cells

   Depositor

   Weiping Han

   Article

   Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-C1 Lifeact-EGFP

   Plasmid

   #58470

   Purpose

   Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Bioarchitecture. 2015 Aug 28:1-14.

   Insert

   Lifeact
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LifeAct-GFP-Fis1

   Plasmid

   #182576

   Purpose

   Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

   Depositor

   Uri Manor

   Article

   Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

   Insert

   LifeAct-GFP-Fis1
   

   Use

   Tags

   Fis1 and LifeAct

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLViP-LifeAct-iRFP670

   Plasmid

   #229699

   Purpose

   Lentiviral expression of LifeAct-iRFP670 in mammalian cells

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   Synthetic construct LifeAct-mTurquoise gene
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lifeact-3xmNG::HIS3

    Plasmid

    #227596

    Purpose

    Integration vector for expressing Lifeact-3xmNeonGreen

    Depositor

    Bruce Goode

    Article

    Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

    Insert

    Lifeact-3xmNeonGreen::HIS3
    

    Use

    Tags

    3xmNeonGreen

    Expression

    Yeast

    Mutation

    Truncated ABP140 (aa 1-17 only)

    Promoter

    Abp140

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LifeAct-GFP-cytoB5RR

    Plasmid

    #182577

    Purpose

    Expresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    LifeAct-GFP-cytoB5RR
    

    Use

    Tags

    LifeAct and cytoB5RR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Tg(mpeg1:LifeAct-mRuby)

    Plasmid

    #166977

    Purpose

    labels F-actin in macrophages

    Depositor

    Anna Huttenlocher

    Article

    Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-380

    Inserts

    mpeg1 (mpeg1.1 Zebrafish)
    LifeAct (actb1 Frog)
    

    Use

    Tags

    Lifeact-mRuby and mRuby

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lifeact-3xmNG::TRP1

    Plasmid

    #227593

    Purpose

    Integration vector for expressing Lifeact-3xmNeonGreen

    Depositor

    Bruce Goode

    Article

    Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

    Insert

    Lifeact-3xmNeonGreen::TRP1
    

    Use

    Tags

    3xmNeonGreen

    Expression

    Yeast

    Mutation

    Truncated ABP140 (aa 1-17 only)

    Promoter

    Abp140

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Tol2-mpx:Lifeact-Ruby

    Plasmid

    #45246

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

    Insert

    lifeact-ruby
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    mpx

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lifeact-3xmNG::URA3

    Plasmid

    #227595

    Purpose

    Integration vector for expressing Lifeact-3xmNeonGreen

    Depositor

    Bruce Goode

    Article

    Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

    Insert

    Lifeact-3xmNeonGreen::URA3
    

    Use

    Tags

    3xmNeonGreen

    Expression

    Yeast

    Mutation

    Truncated ABP140 (aa 1-17 only)

    Promoter

    Abp140

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA_Lifeact-GFP_NLS-mCherry

    Plasmid

    #69058

    Purpose

    Dual Fluorescent Reporter for F-actin and Nuclei

    Depositor

    Olivier Pertz

    Insert

    LifeAct-GFP_IRES_NLS-mCherry
    

    Use

    Tags

    Lifeact-GFP, NLS-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST/LIfeAct-mCherry-N1

    Plasmid

    #40908

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    LifeAct
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    No stop codon to create c-terminal fusion

    Promoter

    CMV

    Available since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lifeact-3xmNG::LEU2

    Plasmid

    #227594

    Purpose

    Integration vector for expressing Lifeact-3xmNeonGreen

    Depositor

    Bruce Goode

    Article

    Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

    Insert

    Lifeact-3xmNeonGreen::LEU2
    

    Use

    Tags

    3xmNeonGreen

    Expression

    Yeast

    Mutation

    Truncated ABP140 (aa 1-17 only)

    Promoter

    Abp140

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-Lifeact EGFP

    Plasmid

    #187686

    Purpose

    In vivo visualization of actin

    Depositor

    Mary Beckerle

    Article

    Smith et al PLoS One. 2013 Aug 21;8(8):e69378. doi:

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMVtrunc sfCherry2ΔC4-Lifeact

    Plasmid

    #231557

    Purpose

    Mammalian expression of Lifeact fused to C-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Lifeact (ABP140 Budding Yeast)
    

    Use

    Tags

    sfCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtrunc

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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