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Showing: 81 - 100 of 583 results

  1. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

    Plasmid
    #140198
    Purpose
    Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    dLbCas12a(D156R)-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Expression
    Mutation
    D832A DNase deactivated, D156R for improved activ…
    Promoter
    PgpdA
    Availability
    Academic Institutions and Nonprofits only
    140198-plasmid-map-sequence-id-309713

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  2. pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

    Plasmid
    #140205
    Purpose
    Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    mCherry
    sgRNA E1
    Use
    CRISPR and Synthetic Biology; Proof-of-concept te…
    Tags
    Expression
    Mutation
    G174D
    Promoter
    PelcA and U3
    Availability
    Academic Institutions and Nonprofits only
    140205-plasmid-map-sequence-id-310180

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  9. pJM483

    Plasmid
    #52987
    Purpose
    A secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensor
    Depositor
    Ding Xue
    Article
    Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d
    Insert
    Lactaherin (Mfge8 Mouse)
    Use
    Tags
    GFP (w/ introns) and signal sequence
    Expression
    Worm
    Mutation
    deleted native signal sequence, deleted C-termina…
    Promoter
    hsp-16.41
    Availability
    Academic Institutions and Nonprofits only
    52987-plasmid-map-sequence-id-82563

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  11. pJM656

    Plasmid
    #53086
    Purpose
    A non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a control
    Depositor
    Ding Xue
    Article
    Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d
    Insert
    Lactaherin (Mfge8 Mouse)
    Use
    Tags
    GFP (w/ introns) and signal sequence
    Expression
    Worm
    Mutation
    deleted native signal sequence, deleted C-termina…
    Promoter
    hsp-16.41
    Availability
    Academic Institutions and Nonprofits only
    53086-plasmid-map-sequence-id-82567

    Enlarge

  12. pSAH51 - [DDp | wrmScarlet | tbb-2 UTR]

    Plasmid
    #200327
    Purpose
    mScarlet BioPart for DD neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [DDp | wrmScarlet | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00007304, ttr-39
    Availability
    Academic Institutions and Nonprofits only
    200327-plasmid-map-sequence-id-397610

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  13. pSAH52 - [DDp | gfp | tbb-2 UTR]

    Plasmid
    #200328
    Purpose
    gfp BioPart for DD neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [DDp | gfp | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00007304, ttr-39
    Availability
    Academic Institutions and Nonprofits only
    200328-plasmid-map-sequence-id-396872

    Enlarge

Showing: 81 - 100 of 583 results