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pBbB14a-GFP

Plasmid

#126117

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMJ915v2 + sfGFP

Plasmid

#88918

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pBbB11a-GFP

Plasmid

#126114

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23150

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-Src-Dasatinib-resistant

Plasmid

#26980

Depositor

Joan Massague

Article

Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

Insert

c-Src (SRC Chicken)

Use

Retroviral

Expression

Mammalian

Mutation

T338I

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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mPapaya1-C1

Plasmid

#54722

Purpose

Localization: C1 Cloning Vector, Excitation: 530, Emission: 541

Depositor

Robert Campbell, Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Type

Empty backbone

Tags

mPapaya1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
pJUMP42x-2A(sfGFP)

Plasmid

#126986

Purpose

Level 2 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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K18iresCreER-T2

Plasmid

#44580

Depositor

Robert Oshima

Article

Wen et al Genesis. 2003 Feb;35(2):100-6.

Insert

keratin 18 gene (KRT18 Human)

Use

Mouse Targeting

Tags

IRES_CreER-T2

Available Since

June 18, 2013

Availability

Academic Institutions and Nonprofits only
pBbB19a-GFP

Plasmid

#126131

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbB18a-GFP

Plasmid

#126133

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB20a-GFP

Plasmid

#126132

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

S23200

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB13a-GFP

Plasmid

#126116

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23102

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbB10a-GFP

Plasmid

#126113

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23116

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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mKO-pBAD

Plasmid

#54735

Purpose

Localization: Protein Expression Vector , Excitation: 548, Emission: 561

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

6xHis and mKO

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pMC-XI2

Plasmid

#176156

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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1xNLS-pMJ915v2

Plasmid

#88915

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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mAzami Green-C1 (mAG)

Plasmid

#54797

Purpose

Localization: C1 Cloning Vector, Excitation: 492, Emission: 505

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al J Biol Chem. 2003 Sep 5. 278(36):34167-7

Type

Empty backbone

Tags

mAzami Green

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
IFP1.4-N1

Plasmid

#54783

Purpose

Localization: N1 Cloning Vector, Excitation: 684, Emission: 708

Depositor

Michael Davidson, Roger Tsien

Article

Shu et al Science. 2009 May 8;324(5928):804-7. doi

Type

Empty backbone

Tags

IFP1.4

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pBbA13a-RFP

Plasmid

#126086

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23102

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pSP72-deltaFlincG

Plasmid

#19733

Depositor

Wolfgang Dostmann

Article

Nausch et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

delta-FlincG (PRKG1 Bovine)

Tags

His

Expression

Bacterial

Mutation

aa 77-356

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pUdOm1.0

Plasmid

#210298

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells with an added 5'UTR

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pY3

Plasmid

#218116

Purpose

Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL636

Plasmid

#189830

Purpose

The T-DNA backbone of Cas12j2 for transcriptional activation in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Promoter

pZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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p221z-AtMIR390a

Plasmid

#118388

Purpose

Entry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

AtMIR390a backbone

Use

RNAi

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB401NL3F10H

Plasmid

#141285

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, KmR for plant)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Luciferase; Gateway

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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mPapaya-N1

Plasmid

#54716

Purpose

Localization: N1 Cloning Vector, Excitation: 530, Emission: 541

Depositor

Robert Campbell, Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Type

Empty backbone

Tags

mPapaya

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only
LII destination

Plasmid

#104529

Purpose

LII backbone vector

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Type

Empty backbone

Use

Ralstonia solanacearum transformation vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pDESTmycTNRC6C

Plasmid

#19874

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6C (TNRC6C Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pBbA19a-RFP

Plasmid

#126101

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-X4

Plasmid

#176154

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-XII1

Plasmid

#176159

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-XI3

Plasmid

#176157

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-XII5

Plasmid

#176161

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ141-ZmUbi-RZ-Aac

Plasmid

#129685

Purpose

(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbS15k-RFP

Plasmid

#126194

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA-GFP

Plasmid

#133009

Purpose

pFA carrying a sfGFP gene

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

sfGFP

Tags

6xhis

Expression

Bacterial

Promoter

tetR

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRD119 (PCNA GFP)

Plasmid

#14822

Depositor

Robert Duronio

Article

Thacker et al Curr Biol. 2003 Jan 8. 13(1):53-8.

Insert

PCNA-GFP (PCNA Fly)

Tags

GFP

Expression

Bacterial

Available Since

July 30, 2007

Availability

Academic Institutions and Nonprofits only
pJUMP22x-1A(sfGFP)

Plasmid

#126969

Purpose

Level 1 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AP582-2

Plasmid

#70049

Purpose

Expresses Ollas::mCherry::linker::H2B::V5 in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

mCherry::linker::H2B

Tags

Ollas and V5

Expression

Bacterial

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK4a_020

Plasmid

#123833

Purpose

Encodes the d4 PEST tag as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

d4PEST tag

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK3a_007

Plasmid

#123760

Purpose

Encodes Peroxisome Targeting Sequence as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PTS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRetroSuper-shYes

Plasmid

#26987

Depositor

Joan Massague

Article

Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

Insert

shRNA Yes (YES1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
epiCRISPRn

Plasmid

#135961

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSNAPf-AP

Plasmid

#61830

Purpose

Encodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPull

Depositor

Aaron Hoskins

Article

Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

Insert

SNAP-AP

Use

Pcr template

Expression

Bacterial and Yeast

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only
FLAG Enthoprotin Full Length

Plasmid

#47419

Depositor

Peter McPherson

Article

Wasiak et al J Cell Biol. 2002 Sep 2;158(5):855-62. d

Insert

Enthoprotin

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pJUMP43-2A(sfGFP)

Plasmid

#126987

Purpose

Level 2 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK4a_019

Plasmid

#123832

Purpose

Encodes the d2 PEST tag as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

d2PEST tag

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSKΔB

Plasmid

#177268

Purpose

Backbone vector for STREAMING-tag knockin vector

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-X2

Plasmid

#176152

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-X3

Plasmid

#176153

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-XI1

Plasmid

#176155

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-1

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2451 - 2500 of 17798 results

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We narrowed to 17,798 results for: bon