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pET-21a(+) SurE

Plasmid

#12669

Depositor

SeWon Suh

Article

Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

Insert

SurE (TM1662 T.maritima)

Tags

His

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
pLII-proUBI10_CKA3 InsPX6 K69A,K71A,K72A,K242A,H271A,K274A-FLAG_termNOS _OLE-RED

Plasmid

#238524

Purpose

Plant expression of flag-tagged A. thaliana CK2alpha3 InsPX6 K69A,K71A,K72A,K242A,H271A,K274A; with OLE1-RED marker

Depositor

Michael Hothorn

Insert

AtCK2alpha3 (AT2G23080 Mustard Weed)

Tags

FLAG

Expression

Plant

Mutation

K69A,K71A,K72A,K242A,H271A,K274A

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pWW2149

Plasmid

#80392

Purpose

Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains.

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Insert

Taz Histidine Kinase

Use

Synthetic Biology

Tags

Fused by GS linkers to 4 SH3 domains

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pVH001

Plasmid

#80394

Purpose

Contains aTc inducible Scaffold-mCherry-AAV and TetR genes

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Insert

mCherry RFP

Use

Synthetic Biology

Tags

Fused by GS linkers to Sh3pep-LZX scaffold protei…

Expression

Bacterial

Available Since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS292

Plasmid

#73725

Purpose

SapTrap 2xNLS-mCherry + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

2xNLS-mCherry + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pTargetF

Plasmid

#62226

Purpose

Constitutive expression of sgRNA without donor editing template DNA

Depositor

Sheng Yang

Article

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Insert

sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pij23119

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_tBFP_PGK_mCherry

Plasmid

#79130

Purpose

Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

tBFP

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_EGFPligand

Plasmid

#79129

Purpose

Lentiviral vector for surface expressed EGFP (SFFV promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

EGFP surface ligand

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCI NL4-3 Nef-HA-WT

Plasmid

#24162

Depositor

Warner Greene

Article

Geleziunas et al Nature. 2001 Apr 12. 410(6830):834-8.

Insert

Nef (nef HIV)

Tags

HA

Expression

Mammalian

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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DAGR

Plasmid

#14865

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

DAG reporter (Prkcb Rat)

Tags

CFP and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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FLAG.PKCzeta.T410E

Plasmid

#10804

Depositor

Alex Toker

Article

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Insert

PKC zeta (Prkcz Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Activation loop active: T410E (Thr 410 to Glu)

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pZH1 Dual G-less cassette

Plasmid

#231645

Purpose

plasmid containing dual G-less cassette for in vitro transcription

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG.PKCzeta.K/W

Plasmid

#10800

Depositor

Alex Toker

Article

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Insert

PKC zeta (Prkcz Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Kinase dead mutant: K281W (Lys 281 to Trp)

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
FLAG.PKCzeta.T410A

Plasmid

#10801

Depositor

Alex Toker

Article

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Insert

PKC zeta (Prkcz Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Activation loop inactive: T410A (Thr 410 to Ala)

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pOL2340

Plasmid

#37841

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Ub-M

Tags

mCherry

Expression

Worm

Mutation

R77M

Promoter

nhx-2

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pMLS262

Plasmid

#73718

Purpose

C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Psnt-1::2xNLS-FLP-D5

Expression

Worm

Mutation

G5D

Promoter

Psnt-1

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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4H4H

Plasmid

#51314

Depositor

Cheryl Arrowsmith

Insert

4H4H (EHMT1 Human, 4H4H)

Tags

His

Expression

Bacterial

Mutation

Truncation: See Comments

Promoter

T7

Available Since

Feb. 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pMLS256

Plasmid

#73715

Purpose

SapTrap destination vector for building combined sgRNA expression + repair template vectors

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

pU6::sgRNA

Use

CRISPR

Expression

Worm

Mutation

SapI insertion site

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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Gal-Rad51

Plasmid

#33230

Depositor

Hannah Klein

Article

Shah et al Mol Cell. 2010 Sep 24;39(6):862-72.

Insert

Rad51 (RAD51 Budding Yeast)

Expression

Yeast

Promoter

Gal1

Available Since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
pMLS260

Plasmid

#73685

Purpose

Punc-17::2xNLS-FLP-D5 Expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Punc-17::2xNLS-FLP-D5::let-858 3'-UTR

Use

Flp/frt

Expression

Worm

Mutation

G5D

Promoter

Punc-17

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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