-
Plasmid#205233PurposeExpresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cellsDepositorInserttwo repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
UseTagsNuclear Export Sequence and mScarlet-iExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSHS212
Plasmid#87346PurposeCysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmidDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/C574SPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab5A S34N
Plasmid#28045DepositorInsertRab5A (RAB5A Human)
UseTagsEGFP on N-termExpressionMammalianMutationS34NPromoterAvailable sinceFeb. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR_CMV_Melt-37-mCherry
Plasmid#234060PurposeEncodes a Melt variant with a switching temperature of 37°CDepositorInsertMelt-37-mCh
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
FCK-ChR2-GFP
Plasmid#15814PurposeLentiviral expression of ChR2-GFP for neuronal excitationDepositorInsertChR2
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCaMKIIAvailable sinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDD120
Plasmid#119879PurposeConstitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.DepositorInsertpConstitutive_Che9c60 and Che9c61
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-EV
Plasmid#202466PurposeEmpty vector of pSMART-HCKanDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-GFP
Plasmid#13772PurposeFlp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.DepositorInsertEGFP
UseFlp/frtTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
RepX_pET28a(+)
Plasmid#172199PurposeExpresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.DepositorInsertRepX superhelicase
UseTags6xHis tagExpressionBacterialMutationC18L, C43S, C167V, S400C, C612APromoterAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB151
Plasmid#101666PurposeOpto-T7RNAP*(563-F1)DepositorInsertOpto-T7RNAP*(563-F1)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB152
Plasmid#101663PurposeOpto-T7RNAP*(563-F2)DepositorInsertOpto-T7RNAP*(563-F2)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-GFP
Plasmid#58867PurposeAAV expression of GFP under the Syn promoterDepositorInsertGFP
UseAAVTagsExpressionMammalianMutationPromoterSynAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-iRFP670-CaaX
Plasmid#170464PurposeExpresses iRFP670 fluorophore targeted to the plasma membrane using a prenylation motifDepositorInsertiRFP670-CaaX
UseLentiviralTagsCaaXExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
KG#908
Plasmid#110914PurposeExpresses the plant proteins TIR1 (necessary for Auxin-Inducible Degradation) pan-neuronally in C. elegansDepositorInsertsrab-3 promoter
TIR1 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDD143
Plasmid#119880PurposeArabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.DepositorInsertpBAD_dCas9sth1
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-ERT2-Cre-ERT2
Plasmid#82587PurposeMiddle entry vector containing tamoxifen-inducible estrogen receptor (ERT2) fused to both ends of CRE recombinase for tamoxifen-inducible Cre recombinationDepositorInsertERT2-CRE-ERT2
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cry2FL-VP64
Plasmid#60554PurposeExpresses Cry2FL-VP64 in mammalian cellsDepositorInsertCry2FL-VP64
UseTagsFLAG, HA tag, and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-GAL4-ERT2-VP16
Plasmid#82588PurposeMiddle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expressionDepositorInsertGAL4-ERT2-VP16
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-GNMT
Plasmid#166177PurposeGateway entry vector for N. furzeri GNMTDepositorArticleInsertGNMT
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-KRAS-G12D
Plasmid#179656PurposeKRAS active mutant (G12D mutation)DepositorInsertKRAS G12D
UseTagsExpressionMammalianMutationG12DPromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only