We narrowed to 1,958 results for: cp

1. M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1

   Plasmid

   #122147

   Purpose

   Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FTH1 (FTH1 Human)
   

   Use

   Lentiviral

   Tags

   NLS-iLID-GFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-IP-mVenus-TOSI

   Plasmid

   #172491

   Purpose

   mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

   Depositor

   David Scadden

   Article

   Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

   Insert

   Pdcd4 (Pdcd4 Mouse)
   

   Use

   Retroviral

   Tags

   mVenus

   Expression

   Mammalian

   Promoter

   MLV LTR

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. M43: pHR-SFFVp-iLID::EGFP::FTH1

   Plasmid

   #122149

   Purpose

   Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FTH1 (FTH1 Human)
   

   Use

   Lentiviral

   Tags

   NLS-iLID-GFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB

   Plasmid

   #122668

   Purpose

   C terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   HNRNPA1 (HNRNPA1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-SspB

   Expression

   Mammalian

   Mutation

   Contains only amino acids 186-230

   Promoter

   SFFV

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTMB352_ZFcharm_Kv1_split-inteins

   Plasmid

   #220848

   Purpose

   Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   ZFcharm Kv2
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTMB301_ZFcharm_Kv1_Prnp_SPM

   Plasmid

   #220840

   Purpose

   AAV genome expressing ZFcharm Kv1 targeting Prnp; single perfect match self-silencing binding site

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   ZFcharm Kv1
   

   Use

   AAV

   Tags

   HAtag

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCVL-UCOE-SFFV-TLR-MCV1

   Plasmid

   #117111

   Purpose

   Lentiviral vector encoding Traffic light reporter 2.0. Alternative name: pCVL-UCOE-SFFV-TLR-MCV1.

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   UCOE SFFV Traffic Light Reporter 2.0
   

   Use

   Lentiviral; Reporter

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRE3G-PE2-P2A-BFP

   Plasmid

   #231580

   Purpose

   Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein

   Depositor

   Jesse Engreitz

   Article

   Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.

   Insert

   PE2-P2A-BFP
   

   Use

   Lentiviral

   Tags

   P2A-BFP

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 IGF1R-BirA(R118G)-HA

   Plasmid

   #109232

   Purpose

   This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).

   Depositor

   Victor Dzau

   Article

   Bareja et al J Biol Chem. 2018 Mar 12. pii: RA118.002

   Insert

   IGF1R (IGF1R Human)
   

   Tags

   BirA(R118G) (highly promiscuous form) and HA

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-EF1a-CD90.2-Rluc_miR

    Plasmid

    #163360

    Purpose

    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a CD90.2 surface marker.

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD90.2 (Thy1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTMB304_ZFcharm_Kv1_Prnp_SCR

    Plasmid

    #220842

    Purpose

    AAV genome expressing ZFcharm Kv1 targeting Prnp; scrambled self-silencing binding site

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm Kv1
    

    Use

    AAV

    Tags

    HAtag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAT077

    Plasmid

    #180511

    Purpose

    Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    PlmCasX
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSico p53

    Plasmid

    #12089

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating p53 shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Insert

    p53 shRNA (Trp53 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Mutation

    EGFP is expressed from this plasmid as a marker, …

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAT100

    Plasmid

    #180512

    Purpose

    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSicoR p53

    Plasmid

    #12090

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF p53 shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Insert

    p53 shRNA (Trp53 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Mutation

    EGFP is expressed from this plasmid as a marker, …

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-Trim46

    Plasmid

    #176402

    Purpose

    TRIM46 tagged with GFP

    Depositor

    Thomas Schwarz, Casper Hoogenraad

    Article

    van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

    Insert

    trim46 (Trim46 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-Trim46

    Plasmid

    #176401

    Purpose

    TRIM46 tagged with mCherry

    Depositor

    Thomas Schwarz, Casper Hoogenraad

    Article

    van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

    Insert

    trim46 (Trim46 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAT527

    Plasmid

    #180514

    Purpose

    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. OA-1136J

    Plasmid

    #153023

    Purpose

    Express His-MBP-TEV-CasRx recombinant protein

    Depositor

    Omar Akbari

    Article

    Brogan et al ACS Sens. 2021 Nov 26;6(11):3957-3966. d

    Insert

    CasRx
    

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-INPP5E-CAAX

    Plasmid

    #202746

    Purpose

    express plasma membrane targeted fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    EGFP:INPP5E(214-644):HRAS(172-189) (HRAS, INPP5E Human)
    

    Expression

    Mammalian

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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