We narrowed to 4,065 results for: p2a

1. pcDNA3.1/CIP2A(1-905) L533E V5 His

   Plasmid

   #119288

   Purpose

   CIP2A L533E V5 overexpression, CIP2A dimer defective mutant

   Depositor

   Jukka Westermarck

   Article

   Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

   Insert

   KIAA1524 (CIP2A Human)
   

   Use

   Tags

   V5 His

   Expression

   Mammalian

   Mutation

   L533E

   Promoter

   T7

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLC-HA active Nedd8-P2A-Hygro

   Plasmid

   #124307

   Purpose

   Lentiviral vector for expression of HA tagged active or C-terminal cleaved Nedd8-P2A-Hygro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   Nedd8 (NEDD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Five C-terminal amino acids missing compared to w…

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. JE301: pMVP (L5-L4) Tir1-P2A-AID

   Plasmid

   #121722

   Purpose

   pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   osTir1-P2A-AID (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   9x myc (C-term on osTIR1)

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-mNeptune2.5-P2A-GFP

   Plasmid

   #197156

   Purpose

   Expresses the protein of mNeptune2.5-P2A-GFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   mNeptune2.5-P2A-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-TagRFP658-P2A-GFP

   Plasmid

   #178971

   Purpose

   Expresses TagRFP658 and GFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   TagRFP658-P2A-GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin

   Plasmid

   #199585

   Purpose

   Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vector

   Depositor

   Michael Lin

   Article

   Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

   Inserts

   ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
   AkaLuc
   Blasticidin
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. RV-Cag-Dio-EYFP-P2A-cMaf

   Plasmid

   #98174

   Purpose

   Retroviral vector encoding Cre-dependent expression of EYFP-P2A-Maf

   Depositor

   Oscar Marin

   Article

   Mi et al Science. 2018 Feb 22. pii: science.aar68

   Insert

   cMaf (codon optimzed) (Maf Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Codon optimized

   Promoter

   CAG

   Available since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

   Plasmid

   #170240

   Purpose

   Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S218D, S222D

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tet-O-FUW-GLI1-P2A-mCherry

   Plasmid

   #203858

   Purpose

   Tet-inducible lentiviral plasmid expressing GLI1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   GLI1 (GLI1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIG-703pUC19-tNGFR-P2A-BACH2

    Plasmid

    #186112

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    BACH2 (BACH2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAdx-CMV-iCre-P2A-copGFP

    Plasmid

    #73350

    Purpose

    Express iCre and GFP

    Depositor

    Kazuhiro Oka

    Insert

    CMV-copGFP-T2A-iCre
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-CreiLOV-P2A-FusionRed

    Plasmid

    #192818

    Purpose

    Expresses CreiLOV with FusionRed tag in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    CreiLOV
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTol2-nbt-Electra1-P2A-mScarlet

    Plasmid

    #196113

    Purpose

    zebrafish expression of nbt-Electra1-P2A-mScarlet

    Depositor

    Reinhard Köster

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    nbt-Electra1-P2A-mScarlet
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK401 (Hygro-P2A-mAID-Nluc)

    Plasmid

    #121196

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-Nluc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIG-712pUC19-tNGFR-P2A-ITCH

    Plasmid

    #186085

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    ITCH (ITCH Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA

    Plasmid

    #74302

    Purpose

    Expression of Magneto2.0-p2A-mCherry in Rohon-Beard sensory neurons of the zebrafish

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Insert

    Magneto2.0 (FTL, FTH1, Trpv4 Rat, Human)
    

    Use

    Zebrafish

    Tags

    FLAG, cmcl2::EGFP, and p2A-mCherry

    Expression

    Mutation

    TRPV4: delta 760-871

    Promoter

    Neurog1

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode

    Plasmid

    #170228

    Purpose

    Barcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    Map2k7-MAPK9 fusion (MAPK9, Map2k7 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Fusion

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tet-O-FUW-MAFB-P2A-mCherry

    Plasmid

    #203866

    Purpose

    Tet-inducible lentiviral plasmid expressing MAFB-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    MAFB (MAFB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMX-hPGK-CD3-P2A-CD90.2

    Plasmid

    #163334

    Purpose

    Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the hPGK promoter

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD3
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBV-luc TOP2A 1kb promoter

    Plasmid

    #138274

    Purpose

    Luciferase reporter plasmid driven by TOP2A promoter

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A promoter sequence
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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