Addgene

Showing: 981 - 1000 of 20864 results

Results per page:

Sort results by:

actc1b-mKate2-CDC42-DN

Plasmid

#109532

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

DN

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTet-O-LHX8-T2A-PuroR

Plasmid

#162350

Purpose

Lentiviral expression of LHX8 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

LHX8 (LHX8 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINK-OTUD5 (OTU, aa 171-358)

Plasmid

#61415

Purpose

Expresses human OTUD5 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD5 (OTUD5 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-170 and aa 359-571.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSHUT4-eYFP

Plasmid

#133094

Purpose

Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI

Depositor

Jens Laurids Sørensen

Article

Nielsen et al Fungal Biol Biotechnol. 2019 Dec 11;6:25

Insert

enhanced yellow flourescent protein

Use

Fungal expression

Tags

Expression

Mutation

Promoter

TEF-1α

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTet-O-KLF7-T2A-PuroR

Plasmid

#162346

Purpose

Lentiviral expression of KLF7 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

KLF7 (KLF7 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINK-OTUD3 (OTU+UBA, aa 52-275)

Plasmid

#61411

Purpose

Expresses human OTUD3 (OTU+UBA domains) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD3 (OTUD3 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-51 and aa 276-398.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag‐mTim3

Plasmid

#49208

Purpose

expresses Flag tagged mouse Tim3

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (Havcr2 Mouse)

Use

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

no Tim3 signal sequence

Promoter

EF1a

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2plus-mVenus1-155-I152L-GGGS

Plasmid

#162613

Purpose

Allows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mVenus

Use

Tags

Expression

Mammalian

Mutation

1-155-I152L

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GA-DEVD-GB-NES

Plasmid

#61020

Purpose

green ddFP based caspase-3 biosensor

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A and ddGFP B

Use

Tags

HindIII and NES sequence LALKLAGLDIGS placed afte…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pTet-O-NEUROG3-T2A-PuroR

Plasmid

#162341

Purpose

Lentiviral expression of NEUROG3 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

NEUROG3 (NEUROG3 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

Plasmid

#61582

Purpose

Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

Cezanne2 (OTUD7A Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Isoform 2. Deleted aa 463-933.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Flag-LmoCas6

Plasmid

#126485

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Flag-LmoCas8b2

Plasmid

#126482

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas8b2

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Flag-LmoCas7

Plasmid

#126483

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas7

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Flag-LmoCas5

Plasmid

#126484

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas5

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2plus-mKate2

Plasmid

#162624

Purpose

Allows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mKate2

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINK-VCPIP (OTU, aa 25-561)

Plasmid

#61583

Purpose

Expresses human VCPIP (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

VCPIP (VCPIP1 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-24 and aa 562-1222.

Promoter

T7

Available since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1 AnxA6

Plasmid

#20407

Depositor

Carlos Enrich

Article

Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

Insert

AnxA6 (Anxa6 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2009

Availability

Academic Institutions and Nonprofits only
p3E-CDC42-DN

Plasmid

#109584

Purpose

Multisite gateway vector for 3' tagging with dominant negative CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

dominant negative

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
actc1b-mKate2-SERCA1

Plasmid

#109498

Purpose

Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

SERCA1 (atp2a1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Please see Depositor Comments

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge