173,291 results
-
Plasmid#164469PurposeConstitutive CAG-driven expression of mGreenLantern in animals using AAVDepositorInsertmGreenLantern
UseAAVExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCAGAvailable SinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
MR015-H11-L2-TALEN
Plasmid#51554PurposeExpresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.DepositorInsertH11-L2 TALEN
UseTALENExpressionMammalianAvailable SinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-VPR-S-L1-dCjCas9 MiniCAFE
Plasmid#169910PurposeExpression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.DepositorInsertMiniCAFE
UseCRISPRExpressionMammalianMutationD8A, HNH-truncation (Δ495–609 aa) in CjCas9PromoterCMVAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HaloAKAR2.2-T2A-EGFP
Plasmid#216490PurposeExpression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cellsDepositorInsertHaloAKAR2.2
TagsT2A-EGFPExpressionMammalianPromoterCMVAvailable SinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAC-lacZ
Plasmid#13422DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/LoxExpressionBacterialAvailable SinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-UFBP1
Plasmid#204558PurposeExpresses GST-tagged UFBP1 in E.coliDepositorAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag TBK1
Plasmid#20648DepositorAvailable SinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
MR015-H11-R2-TALEN
Plasmid#51555PurposeExpresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).DepositorInsertH11-R2 TALEN
UseTALENExpressionMammalianAvailable SinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_repA1
Plasmid#211910PurposeReplicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)
Plasmid#165456PurposeInducible ZEB2 expression from AAVS1 locusDepositorAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJZC519
Plasmid#62278PurposeYeast dCas9-VP64 expression plasmidDepositorInsertdCas9-VP64
TagsHas a VP64 fusionExpressionYeastMutationHas mutations in the RuvC1 and HNH domains to ren…PromoterpTdh3Available SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
HR-TERT-SV40-GFP
Plasmid#71397PurposeHomologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.DepositorInsertTERT (TERT Human)
TagsSV-40 GFP insertion in TERT promotorPromoterEndogenous TERT promotorAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-SAM
Plasmid#102559PurposePiggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.DepositorInsertsMS2-P65-HSF1_T2A_Hygro
dCAS9(D10A, N863A)-VP64_T2A_Blast
UseCRISPR; Piggybac transposonExpressionMammalianMutationD10A and N863A in Cas9 and N55K in MS2PromoterCAG and EF1AAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rInsp-hIns-GLuc
Plasmid#89927PurposeExpresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Human, Synthetic)
UseLentiviralExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…Promoter410bp rat insulin II promoterAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
HRE-oUnaG
Plasmid#201712PurposeHypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1aDepositorInsertUnaG
ExpressionMammalianAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0278
Plasmid#142584PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-HRP-KDEL
Plasmid#85582PurposeExpresses HRP-KDEL in mammalian cellsDepositorInsertHRP-KDEL
TagsV5ExpressionMammalianAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLacO-ISce1
Plasmid#58505Purposeprovides 256 Lac operator repeats adjacent to the ISce1 restriction siteDepositorInsertLac repeats adjacent to 6 copies of the ISce1 restriction site
ExpressionBacterialPromoterno promoterAvailable SinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
BioBrick pET-24a(+)
Plasmid#117802PurposeBioBrick pET vector for protein overexpression in E. coliDepositorTypeEmpty backboneUseEscherichia coliAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-IRES-deltaNGFR
Plasmid#72930Purposeretroviral expression with FACS-selectable marker (extracellular part of NGFR)DepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only