165,688 results

1. AAVS1-tdTomato

   Plasmid

   #159275

   Purpose

   Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11

   Insert

   tdTomato
   

   Use

   AAV; S1 knockin donor vector

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRANS_250d

   Plasmid

   #91124

   Purpose

   Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   PvUbi2

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV-EF1amCherry-2A-OsTIR1-W

   Plasmid

   #159296

   Purpose

   Lentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) system

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   OsTIR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   human EF1a promoter

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28c-F30-Broccoli

   Plasmid

   #66788

   Purpose

   Expresses Broccoli in the F30 scaffold in bacterial cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

   Insert

   Broccoli in the F30 scaffold
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-Impact-T4gp43(exo-)

   Plasmid

   #228202

   Purpose

   Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A

   Depositor

   Stephen Benkovic

   Insert

   43
   

   Tags

   Sce VMA intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   Mutated aspartate 219 to alanine (D219A), elimina…

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCH1/RAD51o

   Plasmid

   #102562

   Purpose

   Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

   Depositor

   Maria Spies

   Article

   Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11

   Inserts

   Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Human, Synthetic)
   E. coli groE operon
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli Expression

   Promoter

   T7

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pABE8e-protein

   Plasmid

   #161788

   Purpose

   Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

   Depositor

   David Liu

   Article

   Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

   Insert

   ABE8e base editor
   

   Tags

   8xHIS and BPNLS

   Expression

   Bacterial

   Promoter

   pRhaBAD

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEmerald-TGNP-N-10

   Plasmid

   #54279

   Purpose

   Localization: Golgi Complex, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   TGNP (TGOLN2 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pPBbsr-mKO-MK2

   Plasmid

   #115492

   Purpose

   Expression vector for the p38 activity reporter mKO-MK2 in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

   Insert

   mKO-MK2 (MAPKAPK2 Human)
   

   Tags

   monomeric Kusabira Orange (mKO)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9-CBE6c-V106W

    Plasmid

    #215826

    Purpose

    Express CBE6c V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6c V106W-SpCas9-2xUGI (cas9 )
    

    Use

    In vitro transcription template

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC57-sgRNA expression vector

    Plasmid

    #51132

    Purpose

    For in vitro transcription of sgRNA from the T7 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. dg263_pENTR_ChrimsonCel

    Plasmid

    #66101

    Purpose

    Entry clone containing Chrimson coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

    Depositor

    Dominique Glauser

    Article

    Schild et al Genetics. 2015 May 28. pii: genetics.115

    Insert

    Chrimson
    

    Use

    Gateway

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-IP TFEB-sfGFP

    Plasmid

    #135402

    Purpose

    Expresses TFEB-sfGFP

    Depositor

    Eisuke Itakura

    Article

    Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

    Insert

    TFEB (TFEB Human)
    

    Use

    Retroviral

    Tags

    sfGFP

    Expression

    Mammalian

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNCS-mRuby3

    Plasmid

    #74234

    Purpose

    Constitutive expression of mRuby3 in bacteria

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    mRuby3
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETSXM2-pLacI-mtrCAB

    Plasmid

    #174618

    Purpose

    Bacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    mtrCAB
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLacI

    Available Since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. MAPK8

    Plasmid

    #73236

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAPK8 (MAPK8 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00069:Pkinase 26-321

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOGG001

    Plasmid

    #113978

    Purpose

    Level 0 module. nptII promoter for constitutive gene expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    nptII promoter
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiV_Neo_LKB1

    Plasmid

    #108111

    Purpose

    Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    LKB1 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS promoter

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDisplay-mSA-EGFP-TM

    Plasmid

    #39863

    Depositor

    Sheldon Park

    Article

    Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

    Insert

    monomeric streptavidin, EGFP, TM helix
    

    Tags

    EGFP, FLAG, and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-L1PA1CH blast

    Plasmid

    #69612

    Purpose

    Expresses a blasticidin tagged codon optimized L1PA1

    Depositor

    Astrid Roy-Engel

    Insert

    L1PA1
    

    Tags

    blast cassette

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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