163,353 results

1. pLV-CAG-FLEX-BFP2-(mCherry)’

   Plasmid

   #115234

   Purpose

   Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

   Depositor

   Ian Wickersham

   Article

   Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

   Insert

   mTagBFP2 and mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-LiLac

   Plasmid

   #184570

   Purpose

   Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV

   Depositor

   Gary Yellen

   Article

   Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

   Insert

   LiLac fluorescent sensor of lactate
   

   Use

   AAV

   Tags

   His7

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX311_CdTag-HA_EmptyVector

   Plasmid

   #221468

   Purpose

   Gateway backbone for C-terminal dTag cloning

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FKBP F36V

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-myc-Miro1

   Plasmid

   #47888

   Purpose

   Expresses myc tagged Miro1

   Depositor

   Pontus Aspenström

   Article

   Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

   Insert

   Miro1 (RHOT1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCF525-EF1a-Hygro-P2A-mCherry-lenti

   Plasmid

   #115796

   Purpose

   Expresses mCherry in human cells, contained in a lentiviral vector.

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF-1a promoter

   Available since

   Oct. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

   Plasmid

   #113865

   Purpose

   Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Inserts

   CMV-eGFP
   TOP-iRFP
   PTCH1-mTurquoise2
   CBF-tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Venus-rab11a

   Plasmid

   #234744

   Purpose

   Mammalian expression of recycling endosome marker Venus-rab11a

   Depositor

   Nevin Lambert

   Article

   Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

   Insert

   Venus-rab11a (RAB11A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe puro c-met WT

   Plasmid

   #17493

   Depositor

   Joan Brugge

   Article

   Wrobel et al J Cell Biol. 2004 Apr 26. 165(2):263-73.

   Insert

   c-Met (Met Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-6xGLY-Myc-hphMX4

   Plasmid

   #20770

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. IDG_TMEM63B_OE_1

    Plasmid

    #161682

    Purpose

    Overexpresses TMEM63B

    Depositor

    Mike McManus

    Insert

    TMEM63B (TMEM63B Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUA66 theta GFP

    Plasmid

    #105607

    Purpose

    Expresses GFP with no promoter

    Depositor

    Pamela Silver

    Article

    Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    No Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-VSVG

    Plasmid

    #1733

    Purpose

    VSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan lab

    Depositor

    Garry Nolan

    Insert

    VSV-G Envelope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWN_U6-B2M-miniGag-Cas9

    Plasmid

    #228958

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-6xGLY-FLAG-hphMX4

    Plasmid

    #20752

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-mRuby2

    Plasmid

    #99123

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3HA-hMIB1

    Plasmid

    #33317

    Depositor

    Vanessa Redecke

    Article

    Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

    Insert

    MIB1 (MIB1 Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD-bARGSer-AxeTxe

    Plasmid

    #192473

    Purpose

    Second-generation bacterial acoustic reporter gene derived from Serratia

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Inserts

    bARGSer
    AxeTxe
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    the gene Ser39006_001280 was deleted from the clu…

    Promoter

    Native promoter from Enterococcus faecium and pBAD

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. SPYtag-Histag-Avidin

    Plasmid

    #214836

    Purpose

    This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    SPYtag-Histag-Avidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-RO1.7-RPE65

    Plasmid

    #206346

    Purpose

    AAV plasmid expressing RPE65 in photoreceptors

    Depositor

    Connie Cepko

    Article

    Xue et al Proc Natl Acad Sci U S A. 2023 Jun 6;120

    Insert

    Rpe65 (Rpe65 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    450-Met (C57BL6 natural variation)

    Promoter

    1.7kb human red opsin

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-EF1-copGFP

    Plasmid

    #73030

    Purpose

    Express copGFP

    Depositor

    Kazuhiro Oka

    Insert

    copGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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