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pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168169

Purpose

Expression of YFP-BeCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Tags

YFP

Expression

Worm

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_CEACAM1

Plasmid

#140102

Purpose

CRISPR-Cas9 library validation

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-dTM-HA

Plasmid

#139846

Purpose

Lentiviral expression of human DDRGK1-dTM-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Mutation

d1-27

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-K267R-HA

Plasmid

#139848

Purpose

Lentiviral expression of human DDRGK1-K267R-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Mutation

K267R

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Flex-rev-GFPFANACfullWT

Plasmid

#126551

Purpose

AAV Flex reverse plasmid with the coding sequence of eGFP fused to the N-term of the FMRFamide gated Na channel

Depositor

Rory McQuiston

Article

Schanuel et al Neuroscience. 2008 Aug 13;155(2):374-86.

Insert

eGFP N-terminal fused to FMRFamide gate sodium channel

Use

AAV

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGS_N_3xFLAG

Plasmid

#101654

Purpose

Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, 3xFLAG tag

Tags

3xFLAG tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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KHBD00550

Plasmid

#39645

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG12245 (gcm Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only
K420CLM RP

Plasmid

#24443

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S; aa 1-420 only

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pLenti6.3-KLRC1-V5

Plasmid

#124093

Purpose

KLRC1-V5 tagged expression vector

Depositor

J. Chad Brenner

Article

Mann et al Oral Oncol. 2023 Nov;146:106562. doi: 10

Insert

KLRC1 (KLRC1 Human)

Use

Lentiviral

Tags

V5

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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A3B-Cas9n-UGI-NLS

Plasmid

#198889

Purpose

Express full-length A3B BE3-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (FANCC)

Plasmid

#180192

Purpose

AAV vector carrying a guide RNA targeting the human FANCC mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (CTNNB1)

Plasmid

#180193

Purpose

AAV vector carrying a guide RNA targeting the human CTNNB1 mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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C1ORF43(1-41)-3xFLAG

Plasmid

#130881

Purpose

Expresses first 41 amino acids of human C1ORF43 with C-terminal 3xFLAG tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

C1ORF43 (C1orf43 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acids 44-253

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestGFP_RBPMS

Plasmid

#65744

Purpose

expresses GFP-tagged RBPMS in mammalian cells

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestRFP_RBPMS

Plasmid

#65745

Purpose

expresses RFP-tagged RBPMS in mammalian cells

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

RFP

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
psicheck-2-LPPRC-mod

Plasmid

#48168

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

LRPPRC leucine rich pentatricopeptide repeat containing (LRPPRC Human)

Use

Luciferase

Mutation

A/T mutation in the ATG of the uORF

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Nnmt in situ probe

Plasmid

#45631

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Nnmt in situ probe (Nnmt Mouse)

Use

In situ

Mutation

fragment contains bp# 454-908 of NM_010924.2

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
A337C

Plasmid

#24432

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
4928 TRV2-TnpB-HHreRNA4PDS

Plasmid

#251815

Purpose

Editing Photene desaturase (PDS) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

ISDra2 TnpB with guide targeting NbPDS flanked by HH-HDV ribozymes

Tags

AtFDnls

Expression

Plant

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pCOLA-A-pC-tac

Plasmid

#213494

Purpose

Encodes Node A of the three-node Turing circuit

Depositor

Mark Isalan

Inserts

rpaI (rpaI )
tetR
4cl
tal

Use

Synthetic Biology

Tags

ASV tag and LVA tag

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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