We narrowed to 20,178 results for: ATO

Showing: 10041 - 10060 of 20178 results

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C234-E08: attB2r-3xFLAG-attB3

Plasmid

#162912

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal triple-FLAG epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

triple FLAG epitope tag

Use

Synthetic Biology

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E44: attB5r-SNAP-nostop-attB1r

Plasmid

#162962

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal SNAP-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SNAP tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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p3.2mar-GFP

Plasmid

#63694

Purpose

3.2 kb marine armor plate enhancer driving GFP reporter

Depositor

David Kingsley

Article

O'Brown et al Elife. 2015 Jan 28;4:e05290. doi: 10.755

Insert

3.2 kb marine stickleback armor plate enhancer

Tags

EGFP

Expression

Mammalian

Promoter

hsp70

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r

Plasmid

#162967

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Dual Strep2 purification tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCFD3-MS2tail-SAM

Plasmid

#78905

Purpose

Vector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoter

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Aug. 27, 2016

Availability

Academic Institutions and Nonprofits only

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CAG-EGFP-Otx2shRNA1

Plasmid

#73981

Purpose

Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse Otx2 gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC1 (1-630)

Plasmid

#22974

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC1 (1-630) (CRTC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 1-630 rather than 1-634.

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pU6-STOP-shA1

Plasmid

#14422

Depositor

Klaus Rajewsky

Article

Oberdoerffer et al Mol Cell Biol. 2005 May . 25(10):3896-90

Insert

modified U6 promoter (U6lox), U6 STOP cassette, shA1 gene

Use

Cre/Lox and RNAi

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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Human NCKX2-AS-long (pcDNA3.1+)

Plasmid

#75201

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Article

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Expression

Mammalian

Mutation

alternatively spliced exon present

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pALPS_3'LTR GFP@gag start SFFV tat

Plasmid

#101338

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized tat

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, tat

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pWPI-NP1

Plasmid

#65060

Purpose

Constitutive expression of NP1

Depositor

Ramon Trullas

Article

Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

Insert

Neuronal Pentraxin 1 (Nptx1 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

* see comments

Promoter

EF1alpha

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pILGFP9E3

Plasmid

#185842

Purpose

Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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BC-A1-005

Plasmid

#78692

Purpose

A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator

Use

Synthetic Biology

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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YFP(1-158)-STOP

Plasmid

#66107

Purpose

This vector attaches YFP(1-158) to the C-terminus of a protein

Depositor

Catherine Berlot

Article

Hu et al J Biol Chem. 2013 Apr 19;288(16):11416-3

Insert

YFP(1-158)STOP

Expression

Mammalian

Mutation

Gln69 was changed to Met.

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pES002 (pTet-qacR wt)

Plasmid

#69031

Purpose

wild-type qacR downstream of a Tet promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

qacR (qacR Synthetic)

Tags

His6 tag

Expression

Bacterial

Promoter

pTet

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pOME0005R

Plasmid

#178374

Purpose

Expresses KSHV ORF K2

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV ORF K2

Expression

Mammalian

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOME0006L

Plasmid

#178376

Purpose

Expresses KSHV K2 ORF tagged

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV ORF K2

Use

Lentiviral

Tags

3xFLAG

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-DEST-R1R2-pNeo

Plasmid

#141057

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Neomycin resistance gene

Expression

Mammalian

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pZF-BCB-GCN

Plasmid

#127476

Purpose

coding sequence for synthetic zinc finger ZF-BCB-GCN

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCB synthetic transcription factor fused with GCN4 leucine zipper

Use

Cell-free transcription–translation

Tags

GCN4 leucine zipper

Promoter

T7

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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Human NCKX2-AS-short (pcDNA3.1+)

Plasmid

#75200

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Article

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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