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pFRT/TO/RFP-DDX54

Plasmid

#97061

Purpose

Expresses RFP (tdTomato)-tagged DDX54 in mammalian cells

Depositor

Markus Landthaler

Article

Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

Insert

DDX54 (DDX54 Human)

Tags

RFP (tdTomato)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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GAL-p300-230-670

Plasmid

#89099

Purpose

mammalian expression of human p300 amino acids 230-670 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 230-670

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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GAL-p300-1601-1900

Plasmid

#89102

Purpose

mammalian expression of human p300 amino acids 1601-1900 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 1601-1900

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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GAL-p300-501-999

Plasmid

#89100

Purpose

mammalian expression of human p300 amino acids 501-999 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 501-999

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pSIL-eGFP-shACTN1

Plasmid

#52676

Purpose

shRNA against α-actinin-1 with eGFP transfection marker

Depositor

Johannes Hell

Article

Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

Insert

ACTN1 (Actn1 Rat)

Use

RNAi

Tags

CMV-EGFP

Promoter

U6

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37deltaSEP

Plasmid

#113501

Purpose

Bacterial expression of GST-tagged p37 lacking the SEP domain

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 140-206

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-p300-DY1399

Plasmid

#89095

Purpose

mammalian expression of full-length human p300 with DY1399 mutation, acetylation mutant, acetyltransferase activity defect

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300-D1399Y mutant (EP300 Human)

Tags

HA

Expression

Mammalian

Mutation

p300-DY1399

Promoter

SV40

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-EFIa-Id1-PGK-puro

Plasmid

#107735

Purpose

Expresses Id1 in mammalian cells

Depositor

Alexandre Colas

Article

Cunningham et al Genes Dev. 2017 Aug 9. pii: gad.300400.1

Insert

Id1 (T Mouse)

Use

Lentiviral

Promoter

EF1a

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pETM41_KPNB1_HEAT

Plasmid

#178494

Purpose

Bacterial expression of human domain with His-tag and MBP tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KPNB1_HEAT (KPNB1 Human, Synthetic)

Tags

His-MBP

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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Sox2-t2A-GFP

Plasmid

#127537

Purpose

Expresses human SOX2 and eGFP via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

SOX2 (SOX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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901 pLNCX myr HA Akt1

Plasmid

#9005

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pET28A-Rab8A

Plasmid

#228880

Purpose

Expression of His-tagged Rab8A in E. coli

Depositor

Sebastian Mathea

Article

Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

Insert

RAB8A (RAB8A Human)

Tags

His tag

Expression

Bacterial

Mutation

residues 1-6 and 177-207 missing

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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BK alpha subunit

Plasmid

#113566

Purpose

mouse BK channel alpha subunit expression vector

Depositor

Robert Brenner

Article

Gruslova et al J Gen Physiol. 2012 Jan;139(1):57-67. do

Insert

Kcnma1 (Kcnma1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

Plasmid

#194279

Purpose

Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

Depositor

Charles Gersbach

Article

McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

Inserts

humanized VP64 dSaCas9 VP64 T2A Thy1.1
gRNA

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hU6 and hUbC

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-PHIP

Plasmid

#65389

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

PHIP (PHIP Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pBS CMV NA 3C FLAG SA

Plasmid

#234993

Purpose

Expression of transmembrane region of Neuraminidase protein fused to streptavidin protein for displaying on viral like particles (VLPs) when cotranfected with GAG protein

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

NA

Tags

HRV 3C site, FLAG, Strep-Tactin (SA)

Expression

Mammalian

Mutation

In the Streptavidin coding sequence Glu44Val/Ser4…

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pNA 3C myc scfvHA

Plasmid

#234991

Purpose

Expression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteins

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

NA-3C-myc-scfvHA

Expression

Mammalian

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DDX54

Plasmid

#97060

Purpose

Expresses FLAG/HA-tagged DDX54 in mammalian cells

Depositor

Markus Landthaler

Article

Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

Insert

DDX54 (DDX54 Human)

Tags

FLAG/HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only
pCI-neo-Flag-DHHS

Plasmid

#85813

Purpose

To rescue the expression of DHHC5 catalytically inactive mutant in the knockdown cell lines

Depositor

Sandra Hofmann

Article

Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

Insert

ZDHHC5 C134S mutant (Zdhhc5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Vcp

Plasmid

#123600

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

valosin containing protein (Vcp Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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