We narrowed to 11,459 results for: 110

1. pME nls EGFP-Cre (JDW 1329)

   Plasmid

   #224515

   Purpose

   A Gateway compatible middle entry clone containing a NLS-EGFP-Cre fusion

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   nls-EGFP-Cre
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME iCre-I (JDW 1203)

   Plasmid

   #224520

   Purpose

   A Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteria

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   iCre-I
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3E-H2A_mCherry_SV40pA (JDW 967)

   Plasmid

   #224528

   Purpose

   A Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   H2A-mCherry-SV40pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)

   Plasmid

   #224532

   Purpose

   A Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   IRES-H2A-mCherry-SV40pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E EFS-rtTA/rtTA3 (JDW 1214)

   Plasmid

   #224533

   Purpose

   A Gateway compatible 3' entry clone containing the Human EFS promoter driving rtTA-3rd generation transactivator

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   rtTA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3E-Actin-Vhh-mNeonGreen-HA (JDW 1225)

   Plasmid

   #224534

   Purpose

   A Gateway compatible 3' entry clone containing an Actin nanobody fused to mNeonGreen with a c-terminal HA tag

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-mNeonGreen-HA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3E-IRES-mTagBFP2-pA (JDW 1361)

   Plasmid

   #224535

   Purpose

   A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   IRES-FLAG-NLS-mTagBFP2-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221-Lifeact- mScarlet-HA (JDW 1247)

   Plasmid

   #224538

   Purpose

   A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Lifeact-mScarlet-HA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME H2B mCerulean (JDW 1150)

   Plasmid

   #224543

   Purpose

   A Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleus

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   H2B-mCerulean
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p5E 5X C120 c-fos min pro (JDW 1241)

    Plasmid

    #224544

    Purpose

    A Gateway compatible 5' entry clone containing 5 TAEL transcription factor binding sites and a minimal c-Fos promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    5x-C120-c-Fos promoter
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-3*flag-NEXN

    Plasmid

    #216834

    Purpose

    expressing Falg tag and the coding sequence of mouse Nexn

    Depositor

    Yuxuan Guo

    Article

    Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

    Insert

    Nexn (mus musculus) (Nexn Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

    Plasmid

    #220494

    Purpose

    To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    sgRNA targeting LIG4 exon 3 (LIG4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

    Plasmid

    #195018

    Purpose

    An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

    Depositor

    Christopher Richie

    Article

    Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

    Inserts

    CCGTATAGGCGGCTGCCCAA
    EGFP-KASH
    TTCCGTGGTGGGCAACGTAG
    

    Use

    AAV

    Tags

    KASH domain of Nesprin2

    Expression

    Mutation

    Promoter

    EF1a, hU6, and mU6

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-APOBEC1-YTHmut-EGFP

    Plasmid

    #209323

    Purpose

    AAV packaging vector containing a APOBEC1-YTHmut expression cassette, a P2A-EGFP expression cassette.

    Depositor

    Magdalena Koziol

    Article

    Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

    Insert

    APOBEC1--YTHmut-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    YTH domain lacks AA 385-409 comprising the m6A-bi…

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. 5'-3xFLAG Zmynd8-exon minimal CMV pCDNA5

    Plasmid

    #212078

    Purpose

    LR vector for integration of Zmynd8-exon into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Zmynd8-exon (Zmynd8 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 5'-3xFLAG Zmynd8+exon minimal CMV pCDNA5

    Plasmid

    #212079

    Purpose

    LR vector for integration of Zmynd8+exon(48nt) into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Zmynd8+exon(48nt) (Zmynd8 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9-RPT6-FLAG

    Plasmid

    #205416

    Purpose

    Expresses dCas9-RPT6 with FLAG label

    Depositor

    Timothy Jarome

    Article

    Farrell et al J Neurosci. 2024 Jan 24;44(4):e145323202

    Insert

    dCas9-RPT6 (Psmc5 Rat)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP Cav2.2 W391A pRK5

    Plasmid

    #206084

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

    Depositor

    Annette Dolphin

    Article

    Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    W391A

    Promoter

    CMV

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cav2.2 HA W391A pcDNA3

    Plasmid

    #206071

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2

    Depositor

    Annette Dolphin

    Article

    Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W391A

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CAGGS-SAFB-NLS-3XFLAG-V5

    Plasmid

    #196089

    Purpose

    Expresses mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB (Safb Mouse)
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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