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pDARMO_CMVT_FLAG-HUWE1

Plasmid

#187121

Purpose

mammalian cell expression of FLAG-HUWE1

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-scCAK1

Plasmid

#177014

Purpose

Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)

Tags

Polyhistidine-TEV

Expression

Insect

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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M13cp-dg3 hp

Plasmid

#218095

Purpose

Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pIII

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Type

Empty backbone

Expression

Bacterial

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-Sox10

Plasmid

#36978

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Sox10 (SOX10 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pCryptDel4.8

Plasmid

#141293

Purpose

Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.

Depositor

Neel Joshi

Article

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Inserts

RelB
gRNA targetting pMUT2

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Native promoter from pMUT2 relB/relE operon and P…

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pKIKOarsBKm

Plasmid

#46766

Purpose

E.coli genomic integration at arsB

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-N LMP2A

Plasmid

#37963

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

LMP2A (LMP-2A H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pTetON-EGFP

Plasmid

#89453

Purpose

Tet/Dox-inducible expression of EGFP in mammalian cells (control)

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

EGFP

Expression

Mammalian

Promoter

TRE3G

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-TMEM106b-mApple

Plasmid

#179385

Purpose

Lentiviral vector for expression of TMEM106b fused mApple

Depositor

Angus Johnston

Article

Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

Insert

TMEM106b - mApple

Use

Lentiviral

Tags

mApple

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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LeGO-iG-CSF2RB

Plasmid

#125750

Purpose

Lentiviral Expression Vektor with CSF2RB wildtype cDNA and IRES eGFP

Depositor

Dirk Heckl

Article

Labuhn et al Cancer Cell. 2019 Aug 12;36(2):123-138.e

Insert

CSF2RB(wt) (CSF2RB 2694, Human)

Use

Lentiviral

Mutation

codon optimized

Promoter

SFFV

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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