164,765 results

1. 3HA-ERHBD-Hoxb8

   Plasmid

   #212932

   Purpose

   Expresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell lines

   Depositor

   Hans Haecker

   Article

   Redecke et al Nat Methods. 2013 Aug;10(8):795-803. doi

   Insert

   3HA-ERHBD-Hoxb8 (ESR1, Hoxb8 Mouse, Human)
   

   Use

   Retroviral

   Tags

   Triple HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-LSSmApple

   Plasmid

   #191259

   Purpose

   Bacterial expression plasmids for Long Stokes shift red fluorescent protein LSSmApple

   Depositor

   Robert Campbell

   Article

   Ejike et al Biochemistry. 2024 Jan 2;63(1):171-180.

   Insert

   LSSmApple
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

   Plasmid

   #172982

   Purpose

   control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

   Plasmid

   #171129

   Purpose

   LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

   Depositor

   Benjamin Kleinstiver

   Article

   Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

   Insert

   U6 promoter entry plasmid for LwaCas13a crRNA cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. moxCerulean3

   Plasmid

   #68066

   Purpose

   C-terminal fusion to moxCerulean3

   Depositor

   Erik Snapp

   Article

   Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

   Type

   Empty backbone

   Use

   Tags

   moxCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRES-H3.3-G34R-FLAG-BFP

   Plasmid

   #206140

   Purpose

   Expression of a FLAG tagged human histone mutant H3.3-G34R with BFP

   Depositor

   Maria Castro

   Insert

   H3.3 (H3-3A Human)
   

   Use

   Tags

   FLAG-eBFP

   Expression

   Mammalian

   Mutation

   G34R

   Promoter

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX-MCS PGK promoter

   Plasmid

   #128044

   Purpose

   Lentiviral vector with PGK promoter

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSSRB069_pAC8-hsDDB1dB

   Plasmid

   #218790

   Purpose

   Insect cell expression vector for 6xHis-TEV-hsDDB1∆B

   Depositor

   Eric Fischer

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   DNA damage-binding protein 1 (DDB1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Insect

   Mutation

   Deleted amino acids 396-705 and replaced with GNG…

   Promoter

   polyhedrin

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-SYN-eGFP-Kindlin 1

   Plasmid

   #203733

   Purpose

   AAV vector plasmid expressing mouse kindlin-1 fused to eGFP under the human synapsin (SYN) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Insert

   Fermt1 (Fermt1 Mouse)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Human synapsin promoter

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHBintE

    Plasmid

    #48136

    Purpose

    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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