We narrowed to 8,810 results for: CAG

1. CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A APGST XE

   Plasmid

   #113848

   Purpose

   Histone H2B-superfolder GFP and tdTomato linked by T2A (APGST linker)

   Depositor

   Mario Capecchi

   Insert

   H2B-sfGFP-T2A-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CAG-loxP-3xpA-sfGFP-tdTomato T2A APGST -XE

   Plasmid

   #113850

   Purpose

   Superfolder GFP and tdTomato linked by T2A (APGST linker, -XE)

   Depositor

   Mario Capecchi

   Insert

   sfGFP-T2A-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216246

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgAAVS1.5
   

   Use

   CRISPR; Human safe harbor locus (a avs1) site-spe…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216251

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgSEC61B.N1 (SEC61B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

   Plasmid

   #104567

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
   

   Use

   CRISPR

   Tags

   NLS-3xHA-VPR

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE

   Plasmid

   #105323

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expression

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7c variant 1513
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)

   Plasmid

   #114078

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR

   Plasmid

   #157744

   Purpose

   knock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expression

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   chlorotoxin containing chimeric antigen receptor targeting glioblastoma
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

   Plasmid

   #85041

   Purpose

   This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   loxP-stop-loxP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-lox-rox-STOP-rox-tRFP-pA-lox-ZsGreen-pA

    Plasmid

    #175438

    Purpose

    dual plasmid labelling cells of different neuronal population with different fluorophores

    Depositor

    Sebastián Dupraz, Frank Bradke

    Article

    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

    Insert

    rox-STOP-rox-tRFP-pA
    

    Use

    Cre/Lox; Dre/rox

    Tags

    ZsGreen on backbone

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid

    #180152

    Purpose

    tdTomato cre reporter with Rosa26 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag

    Plasmid

    #199457

    Purpose

    Expression of dCas13-VPR in mammalian cells

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    dCas13
    

    Use

    Lentiviral

    Tags

    VPR-T2A-HaloTag

    Expression

    Mammalian

    Mutation

    Nuclease-dead H133A and H1058A

    Promoter

    CAG

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

    Plasmid

    #52419

    Purpose

    Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    ERAS (ERAS Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

    Plasmid

    #168106

    Purpose

    PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Insert

    mCherry-KASH
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

    Plasmid

    #68719

    Purpose

    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

    Depositor

    Mark Huebener, Tobias Rose, Tobias Bonhoeffer

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Has Service

    AAV1

    Insert

    mRuby2-P2A-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG-FLEX

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid

    #68717

    Purpose

    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

    Depositor

    Mark Huebener, Tobias Rose, Tobias Bonhoeffer

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Has Service

    AAV1

    Insert

    mRuby2-P2A-GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Cre-dependent expression from inverted open readi…

    Promoter

    CAG-FLEX

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid

    #180156

    Purpose

    tdTomato cre reporter with Hipp11 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA

    Plasmid

    #216321

    Purpose

    Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Luciferase + splice acceptor site
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216249

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgRANGAP.C1 (RANGAP1 Human)
    

    Use

    CRISPR; Endogenous tagging

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1

    Plasmid

    #196890

    Purpose

    Neuron-specific expression of human (h)Centrin fused to mNeonGreen

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mNeonGreen-hCentrin1 (CETN1 Human)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

    Plasmid

    #180153

    Purpose

    td-sfGFP dre reporter with Rosa26 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    td-sfGFP
    

    Use

    Mouse Targeting; Dre/rox

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA

    Plasmid

    #108833

    Purpose

    Expresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDs

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment aa28 to aa396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS and GAI

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark Citrine

    Plasmid

    #104122

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    spine-jRGECO1a-P2A-QuasAr-dark Citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CAG-loxP-3xpA-loxP-tdTomato+RbG and HsbG syntrons

    Plasmid

    #172479

    Purpose

    tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)

    Plasmid

    #114094

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-RR (S542R/K607R)
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    S542R and K607R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAVS1-Puro CAG-CLTX CD32a ITAM CD3z CAR

    Plasmid

    #171965

    Purpose

    Donor plasmid for CLTX CD32a-ITAM CD3z CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

    Insert

    Synthetic CLTX CD32a ITAM CD3z CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pCAGGS

    Plasmid

    #206115

    Purpose

    expression of rat Cav2.1 with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A, E670A, E1411A, E1707A

    Promoter

    CMV/B-actin

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDH-Lenti-CAGGS-hPRDM6-2xGly-V5-EF1-Luc2-Blast

    Plasmid

    #213826

    Purpose

    Expresses human PRDM6 with a C-terminal V5 epitope tag.

    Depositor

    Bjoern Schwer

    Article

    Schmidt et al bioRxiv. 2023 Aug 31:2023.08.29.555389.

    Insert

    PRDM6 (PRDM6 Human)
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-lox-inv[Talpha1-Dre-PA]-lox-N-Cadherin-2xHA

    Plasmid

    #196896

    Purpose

    Neuron-specific expression of N-Cadherin fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    N-Cadherin-2xHA (Cdh1 Mouse)
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry

    Plasmid

    #114726

    Purpose

    dCas9-VP160 expression in mammalian cells

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    dCas9-VP160
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65

    Plasmid

    #104566

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1(D832A)-p65
    

    Use

    Tags

    3x HA and NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. R26-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid

    #180151

    Purpose

    td-sfGFP cre reporter with Rosa26 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    td-sfGFP
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Lyn-EGFP

    Plasmid

    #196875

    Purpose

    Neuron-specific expression of LynGFP reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    LynEGFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

    Plasmid

    #114091

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a (E174R/S542R)
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    E174R and S542R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

    Plasmid

    #112211

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    C-terminus of dCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. H11-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

    Plasmid

    #180157

    Purpose

    td-sfGFP dre reporter with Hipp11 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    td-sfGFP
    

    Use

    Mouse Targeting; Dre/rox

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. H11-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid

    #180155

    Purpose

    td-sfGFP cre reporter with Hipp11 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    td-sfGFP
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPB-CAG-KRAB-dCas9-P2A-mCherry-pgk-hph

    Plasmid

    #233067

    Purpose

    Piggybac CRISPRi construct

    Depositor

    Markus Landthaler

    Article

    Vučićević et al bioRxiv 2024.09.12.612198

    Insert

    KRAB-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAGEN nls-BFP-FLAG-WPRE-bgh-pA (JDW 452)

    Plasmid

    #229827

    Purpose

    A CAGGS driven nls BFP with a FLAG tag followed by a WPRE-bGH polyA for stable transcripts

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    3xnls-TagBFP2-MCS-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-CAG-SNAP-p53DD-IRES-HRAS(G12V)-bGH

    Plasmid

    #233204

    Purpose

    AAV expression of SNAP-p53DD and HRAS(G12V)

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10, Volume 16, Issue

    Insert

    SNAP-p53DD-IRES-HRAS(G12V) (Trp53, HRAS Mouse, Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCM100_ PB5'IR-hU6-gRNA-CS1- CAG-tdTomato- PB3'IR

    Plasmid

    #229995

    Purpose

    PiggyBac sgRNA cloning plasmid with tdTomato reporter with a capture sequence (cs1)

    Depositor

    Christian Mayer

    Article

    Dvoretskova et al Nat Neurosci. 2024 May;27(5):862-872. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    NotI site for NEBuilder HiFi DNA Assembly with ss…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry

    Plasmid

    #232244

    Purpose

    Mammalian expression of N581K RLIM-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    N581K RLIM-GFP-IRES-mCherry (RLIM Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    N581K

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCAGGS puro -GFP-I77P I78P REX1-IRES-mCherry

    Plasmid

    #232245

    Purpose

    Mammalian expression of I77P I78P REX1-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    I77P I78P REX1-GFP-IRES-mCherry (Zfp42 Mouse)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    I77P I78P

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #222858

    Purpose

    This plasmid codes for the Slug-KH nickase with a sg(CAG)6

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    Slug-KHD10A+sgCAG
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, ITR2 and ITR2

    Expression

    Mammalian

    Mutation

    KH variant and D10A mutation

    Promoter

    H1 and eCMV

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCAGGS-lyn10-hLOV-ssrA-P2A-LOVPLD-mCherry-sspB

    Plasmid

    #219557

    Purpose

    PM-targeted iLID-LOVPLD

    Depositor

    Jeremy Baskin

    Article

    Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

    Insert

    lyn10-iLID-LOVPLD
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCAG-GST-ATG13 (363-398)-F394D/M396D/F397D

    Plasmid

    #210848

    Purpose

    Mammalian expression of ATG13 (363-398) mutant

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F394D/M396D/F397D

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCAG-GST-ATG13 (363-450)-F394D/F397D /E403K

    Plasmid

    #210850

    Purpose

    Mammalian expression of ATG13 (363-450) mutant

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F394D/F397D /E403K

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS

    Plasmid

    #206098

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E314A, E664A, E1370A, E1658A

    Promoter

    CMV/B-actin

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCAG-lox-inv[Talpha1-HA-Dre]-lox-Akna-mOrange2

    Plasmid

    #196893

    Purpose

    Neuron-specific expression of Akna fused to mOrange2. Used in combination with Talpha1-iCre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Akna-mOrange2 (Akna Mouse)
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Akna-mOrange2

    Plasmid

    #196880

    Purpose

    Neuron-specific expression of the centrosomal protein Akna fused to mOrange2

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Akna-mOrange2 (Akna Mouse)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge