We narrowed to 7,221 results for: ALP

1. pHAGE2-EF1alpha-HA-TFAP4(E135A/S139A)

   Plasmid

   #110159

   Purpose

   Lentiviral vector for expression of human TFAP4 (E135A/S139A) in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

   Insert

   TFAP4 (transcription factor AP-4) (TFAP4 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E135A/S139A

   Promoter

   CMV

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSG5-FLAG-CaMKKbeta with CaMKKalpha RP domain

   Plasmid

   #33332

   Depositor

   Anthony Means

   Article

   Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

   Insert

   Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   CaMKKbeta RP domain (203-225) replaced with CaMKK…

   Promoter

   SV40

   Available since

   April 17, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pSG5-FLAG-CaMKKbeta N-terminus on CaMKKalpha

   Plasmid

   #33330

   Depositor

   Anthony Means

   Article

   Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

   Insert

   Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   CaMKKbeta N-terminus (1-164) was fused to body fo…

   Promoter

   SV40

   Available since

   April 17, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pSG5-FLAG-CaMKKalpha N-terminus on CaMKKbeta

   Plasmid

   #33329

   Depositor

   Anthony Means

   Article

   Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

   Insert

   Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   CaMKKalpha N-terminus(1-128) was fused with CaMKK…

   Promoter

   SV40

   Available since

   April 17, 2012

   Availability

   Academic Institutions and Nonprofits only

5. Venus Cam KII alpha 1-315aa

   Plasmid

   #29431

   Depositor

   Steven Vogel

   Article

   Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

   Insert

   Venus CAM KII Alpha
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Expresses CamKIIa amino acids 1-315

   Promoter

   Available since

   July 5, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag

   Plasmid

   #179719

   Purpose

   Lentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag

   Plasmid

   #179723

   Purpose

   Lentiviral expression of lysosome-targeted Halo tagged mouse Syt7

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYT7 (Syt7 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pU6-sgRNA EF1Alpha-puro-T2A-BFP

   Plasmid

   #60955

   Purpose

   Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

   Plasmid

   #128755

   Purpose

   Expresses CYRANO gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   CYRANO sgRNA (OIP5-AS1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBabe-GFP-IKBalpha-mut (super repressor)

    Plasmid

    #15264

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKB alpha (NFKBIA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Serine 32 to Alanine, Serine 36 to Alanine

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pU6-sgRNA Ef1alpha Puro-T2A-GFP

    Plasmid

    #111596

    Purpose

    sgRNA Ef1alpha Puro-T2A-GFP

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

    Insert

    mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

    Plasmid

    #141395

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R]

    Plasmid

    #177486

    Purpose

    Mammalian Expression Plasmid of anti-Slo1/BKAlpha maxi-K+ channel (Mouse). Derived from hybridoma L6/60.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Slo1/BKAlpha maxi-K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnma1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBabe-Puro-IKBalpha-mut (super repressor)

    Plasmid

    #15291

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    inhibitor of Kappa B alpha (NFKBIA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S32A/S36A: “super-repressor” allele (resistant to…

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)

    Plasmid

    #188385

    Purpose

    Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro

    Plasmid

    #185451

    Purpose

    Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

    Insert

    Nucleocapsid (N SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAdTrack-CMV FLAG Rev-Erb alpha

    Plasmid

    #22745

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    Rev Erb alpha (NR1D1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PGC-1 alpha promoter luciferase delta CRE

    Plasmid

    #8888

    Depositor

    Bruce Spiegelman

    Article

    Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

    Insert

    PGC-1 alpha promoter dCRE (Ppargc1a Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Site-directed mutagenesis to remove the CREB bind…

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)

    Plasmid

    #225433

    Purpose

    Transcriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.

    Depositor

    Diego Orzaez

    Insert

    4xCBS:mDFR:Rep/RepA:Tnos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO

    Plasmid

    #204340

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    Galpha-s/Gbetagamma ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge