We narrowed to 4,209 results for: Abo;

1. pET28a-Ec.coaE (pESC124)

   Plasmid

   #50390

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Tadhg Begley, Erick Strauss

   Article

   Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

   Insert

   DPCK
   

   Use

   Tags

   6XHIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJKR-H-cdaR

   Plasmid

   #62557

   Purpose

   Glucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.

   Depositor

   George Church

   Article

   Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

   Inserts

   beta lactamase
   sfGFP
   CdaR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKL07

   Plasmid

   #104431

   Purpose

   mitochondrial matrix targeted super ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vector

   Depositor

   Joel Kralj

   Article

   Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

   Insert

   mito-Super Ecliptic pHluorin-mRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tef1

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB8644

   Plasmid

   #126919

   Purpose

   Plasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYB

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   XdCrtI and HMG1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9

   Plasmid

   #162606

   Purpose

   Pre-express Cas9 in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbA5c-MevT-MBI (JBEI-3085)

   Plasmid

   #87950

   Purpose

   the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).

   Depositor

   Taek Soon Lee

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Insert

   MevT-MBI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKL09-GCaMP6f-mScarlet bb118

   Plasmid

   #158979

   Purpose

   Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

   Insert

   GCaMP6f-mScarlet fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   codon optimized for e. coli from Addgene#100833

   Promoter

   Bba_J23118 (sigma 70)

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUCBB-oriT

   Plasmid

   #32556

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   oriT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   N/A

   Available since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-GluA1-scFv-Halo

   Plasmid

   #230055

   Purpose

   AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA1 single chain variable fragment (scFv)
   

   Use

   AAV

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB8787

    Plasmid

    #126921

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of HMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    HMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB8748

    Plasmid

    #126918

    Purpose

    Plasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtE

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtE
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDD363

    Plasmid

    #91829

    Purpose

    LoxP-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    LoxP-SEC-LoxP
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDD379

    Plasmid

    #91834

    Purpose

    SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCfB8788

    Plasmid

    #126922

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI and HMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUW-RLuc-T2A-PuroR

    Plasmid

    #102320

    Purpose

    expression of r-luciferase

    Depositor

    Rudolf Jaenisch

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    Renilla luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ubc

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDD364

    Plasmid

    #91831

    Purpose

    Lox511I-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Lox511I-SEC-Lox511I
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDD382

    Plasmid

    #91830

    Purpose

    Lox2272-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Lox2272-SEC-Lox2272
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. peZinCh-2

    Plasmid

    #70008

    Purpose

    expresses eZinCh-2 Zn2+ FRET sensor in the cytosol of mammalian cells

    Depositor

    Maarten Merkx

    Article

    Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

    Insert

    eZinCh-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. 2xNLS-pMJ915v2

    Plasmid

    #88916

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-EF1a-V5-LIC

    Plasmid

    #120247

    Purpose

    For LIC cloning with N-terminal V5 tag

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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