We narrowed to 16,871 results for: SHE

1. pBAD-LSSmCherry1

   Plasmid

   #89986

   Purpose

   Long stokes-shift variant of red fluorescent protein mCherry

   Depositor

   Robert Campbell

   Article

   Shen et al PLoS One. 2017 Feb 27;12(2):e0171257. do

   Insert

   LSSmCherry1
   

   Use

   Tags

   6xHis-T7-Xpress

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-KIRIN1

   Plasmid

   #107414

   Purpose

   Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbAW4k-loxP-TT-loxP-mRFP1

   Plasmid

   #134405

   Purpose

   Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

   Depositor

   Mary Dunlop

   Article

   Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

   Insert

   W4-loxP-TT-loxP-mRFP1
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   W4 (modified T7A1)

   Available since

   Jan. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. NLS-Halo

   Plasmid

   #200197

   Purpose

   Expression for SV40 NLS

   Depositor

   Sheila Teves

   Article

   Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

   Insert

   3X SV40 NLS-Halo
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   None

   Promoter

   EF_1alpha

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF_dCas9

   Plasmid

   #68416

   Purpose

   Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   human EF1[alpha]

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Phat2-dr-cPla2-C2-EGFP-6H

   Plasmid

   #187001

   Purpose

   Protein Expression

   Depositor

   Philipp Niethammer

   Article

   Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

   Insert

   ENSDARG00000024546 (pla2g4aa Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUBC_V5_MS2-mCherry

   Plasmid

   #68420

   Purpose

   Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   MS2
   

   Use

   Synthetic Biology

   Tags

   V5 and mCherry

   Expression

   Mammalian

   Mutation

   MS2: V75E/A81G

   Promoter

   human UbC

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM5411

   Plasmid

   #132663

   Purpose

   RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Inserts

   aadA
   YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PaadA and PconII

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. MLV Gag-RFP

   Plasmid

   #1814

   Depositor

   Walther Mothes

   Article

   Sherer et al Traffic 2003 Nov;4(11):785-801.

   Insert

   Gag (gag MuLV)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCambia-PUP-IT

    Plasmid

    #186478

    Purpose

    Expresses POI-PUP-IT fusion in Plants

    Depositor

    Jen Sheen

    Article

    Ye et al Nature. 2022 Sep 14. pii: 10.1038/s41586

    Insert

    FLAG-Pup(E)
    

    Use

    Tags

    Triple Flag Tag

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. NES-Halo

    Plasmid

    #200201

    Purpose

    Expression of 3X HIV NES

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    3X HIV Rev NES-Halo
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Friend MLV Env YFP

    Plasmid

    #1820

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    Envelope (env MuLV)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-KIRIN1-GR

    Plasmid

    #107418

    Purpose

    Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1-GR

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    KIRIN1-GR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-LambdaN-NbALFA

    Plasmid

    #207627

    Purpose

    Expresses LambdaN-NbALFA (ALFA-tag nanobody) fusion protein in mammalian cells (with a CMV promoter)

    Depositor

    Jay Shendure

    Article

    Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

    Insert

    LambdaN-NbALFA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pCMV

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFlpStop-HDR-UAS-2.1-tdTom

    Plasmid

    #89148

    Purpose

    FlpStop plasmid for CRISPR/HDR. Has MCS for homology arms

    Depositor

    Tom Clandinin

    Article

    Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    5XUAS, 3XP3-dsRed

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSUPER.retro.puro-shNC

    Plasmid

    #113535

    Purpose

    Control retroviral vector.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    shNC (scrambled control shRNA)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SARS-CoV-2 Spike-d18

    Plasmid

    #164583

    Purpose

    A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

    Depositor

    Jason Sheltzer

    Insert

    SARS-CoV-2 Spike (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1256-1273

    Promoter

    EF1a

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRAF-MutL

    Plasmid

    #86607

    Purpose

    E. coli MutL expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutL
    

    Use

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRAF-MutH

    Plasmid

    #86609

    Purpose

    E. coli MutH expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutH
    

    Use

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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