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pHMattR3-attR4-GFP

Plasmid

#196247

Purpose

pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP gene

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA-SV40-mCherry

Plasmid

#240538

Purpose

Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with mCherry. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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TAF3 PHD_pGEX-6P-2

Plasmid

#92100

Purpose

Expresses GST fusion TAF3 PHD domain protein

Depositor

Albert Jeltsch

Article

Kungulovski et al Epigenetics Chromatin. 2016 Mar 22;9:11.

Insert

TAF3 PHD domain (TAF3 Human)

Tags

GST Tag

Expression

Bacterial

Promoter

tac promoter

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSU-araC-Cas9

Plasmid

#120423

Purpose

Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system

Depositor

Sheng Yang

Article

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLG033 (ietAS)

Plasmid

#157911

Purpose

Expresses the ietAS defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

ietAS (ATPase + serine protease)

Expression

Bacterial

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pACλCI-β-flap (831-1057)

Plasmid

#53733

Purpose

Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

Depositor

Ann Hochschild

Article

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Insert

λCI-β-flap fusion

Expression

Bacterial

Promoter

lac UV5

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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ykgMO-IGS-LuxCDABE

Plasmid

#207232

Purpose

Calprotectin sensing promoter with luciferase reporter

Depositor

Arthur Prindle

Article

Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

ykgMO-IGS

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pJH773_pTech_PlldR-HHRz-supP(opal)

Plasmid

#163732

Purpose

Lactate-inducible expression of supP(opal), preceded by a hammerhead ribozyme

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

hhrz-supP

Expression

Bacterial

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLG014 (RADAR)

Plasmid

#157892

Purpose

Expresses the RADAR defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

RADAR

Expression

Bacterial

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBA620

Plasmid

#186240

Purpose

LbuCas13a Negative Control (RFP targeting)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

pTet-LbuCas13a + crRNA negative control

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pTOX5

Plasmid

#127450

Purpose

Encodes MqsR toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

mqsR toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pHelper_TetR/pTet-Tnpase (camR)

Plasmid

#213910

Purpose

ATc-inducible himar1C9 transposase expression - cam resistance

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

TetR/ptet ATc-inducible himar1C9 transposase

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLG034 (restriction-like)

Plasmid

#157912

Purpose

Expresses a restriction-like defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

4-gene restriction-like system

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLG026 (tmn)

Plasmid

#157904

Purpose

Expresses the tmn defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

tmn (transmembrane ATPase)

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBA630

Plasmid

#186241

Purpose

RfxCas13d Negative Control (RFP targeting)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

pTet-RfxCas13d + crRNA negative control

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGG-BC-TurboID-GOI

Plasmid

#209390

Purpose

Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Expression

Plant

Mutation

TurboID gene sequence C249A

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGG-GOI-DE-TurboID

Plasmid

#209388

Purpose

Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Expression

Plant

Mutation

TurboID gene sequence C249A

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBAC-BA-lacZ

Plasmid

#13423

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

BA

Use

Cre/Lox

Expression

Bacterial

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pVoPo-mNG

Plasmid

#187843

Purpose

Constitutive mNeonGreen-expression vector for Fusobacterium nucleatum

Depositor

Jörg Vogel

Article

Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

Insert

mNeonGreen

Expression

Bacterial

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRN12

Plasmid

#84456

Purpose

shuttle plasmid for sarA P1-mAmetrine expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mAmetrine

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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