We narrowed to 10,500 results for: fus
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Plasmid#131376PurposeLow level expression of E.coli G.stearothermophillus MutY chimera proteinDepositorInsertEcNGsC MutY chimera
ExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-Lyn-AktAR2-D
Plasmid#125198PurposeSB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporterDepositorInsertLyn-AktAR2-D
UseTransposonTagsLyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]ExpressionMammalianMutationFoxO1 Thr-24 changed to Val rendering this inacti…PromoterEF1a/RPBSAAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pProEx-Htb-cytATL2-6Gly-sfGFP
Plasmid#124065PurposeE. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.DepositorInsertcytATL-sfGFP
TagssfGFPExpressionBacterialPromotertrcAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416) (I325D)
Plasmid#121412PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
ExpressionYeastMutationIsoleucine 325 to Aspartic acidPromoterCUP1 promoterAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416) (F317D)
Plasmid#121411PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
ExpressionYeastMutationPhenylalanine 317 to Aspartic acidPromoterCUP1 promoterAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416) (L105D)
Plasmid#121410PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
ExpressionYeastMutationLeucine 105 to Aspartic acidPromoterCUP1 promoterAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-Atg17(416) (L105D)
Plasmid#121405PurposeExpressing Atg17DepositorInsertCUP1p-ATG17 ORF-CYC1 terminator
ExpressionYeastMutationLeucine 105 to Aspartic acidPromoterCUP1 promoterAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
hASXL(1-115)-luciferase
Plasmid#106427PurposeExpresses human ASXL1 (amino acids 1-115) fused to the N-terminal of firefly luciferaseDepositorInsertaddition sex combs like 1 ASXL1 (ASXL1 Human)
TagsluciferaseExpressionMammalianMutationamino acids 1-115 fused to luciferaseAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSN29
Plasmid#100114Purposeexpresses a chimeric cMYC-repressor fusionDepositorUseLentiviralExpressionMammalianAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD1cR
Plasmid#86970PurposeExpresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coliDepositorInsertPCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
TagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, G108C in PCNA1PromoterT7 promoterAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJOG251
Plasmid#80583Purposerecipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTADepositorInsertspnos:PAT-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
BsaI-ccdB_CmR-BsaI
UseCRISPRExpressionPlantMutationD10AAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry
Plasmid#69233PurposeR1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)DepositorInsertSpCas9
UseCRISPRTags2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…ExpressionMammalianMutationArginine 1335 is changed to LysineAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3
Plasmid#69231PurposeExpresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cellDepositorInsertTS3 TALE
UseCRISPRTags2X NLS and NLS-3XHA-NLSExpressionMammalianAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB682
Plasmid#60143PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationA4VPromoterMET17prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWPT-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49227PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
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mycUb-20aa
Plasmid#20255DepositorInsertUbiquitin-20 amino acid extension (RBX1 Human)
TagsmycExpressionMammalianMutationUbiquitin fused to N-terminus of GFP. Glycine 76 …Available SinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pG1-NMGR
Plasmid#1274DepositorAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSP148
Plasmid#247110PurposeExpresses estrogen-binding domain-GFP-nanobody-Rnf4 fusion protein in Aspergillus fumigatusDepositorInsertsUseAffinity Reagent/ Antibody; Maintenance in bacter…Tags4X HAExpressionBacterialAvailable SinceJune 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-copGFP-RBBP6 NTD+IDR4
Plasmid#253989PurposeTransient expression of RBBP6 NTD and IDR4DepositorInsertRBBP6 (RBBP6 Human)
TagscopGFPExpressionMammalianMutationincludes NTD fused to IDR4 only (aa 351-1681 dele…PromoterCMVAvailable SinceMay 14, 2026AvailabilityAcademic Institutions and Nonprofits only