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pL-CRISPR.EFS.GFP

Plasmid

#57818

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

EFS and hU6

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLX302

Plasmid

#25896

Purpose

3rd generation lentiviral Gateway destination vector. Puromycin selection, V5 tag.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI-hTFR1-4

Plasmid

#218799

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

AAV Rep-Cap BI-hTFR1-4

Expression

Mammalian

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1

Plasmid

#237661

Purpose

For integration of EGFP-NPM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1 (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-L274GMmMetRS-T2A-mCherry

Plasmid

#220800

Purpose

Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

Depositor

Elizabeth Kirby

Article

Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

Insert

Mars1 (Mars1 Mouse)

Use

Lentiviral

Tags

2xFLAG and T2A-mCherry

Mutation

mutation in MetRS to change aa 274 from L to G

Promoter

EF1a

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pE-35S-miniSdd7-nCas9-PBE-GmPPO2

Plasmid

#204855

Purpose

For cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd7-nSpCas9(D10A)-UGI-mScarlet

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Promoter

35S, AtU6-26p

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-PD-1 (Y223F, Y248F)-mGFP

Plasmid

#180790

Purpose

Expression of human PD-1 (Y223F, Y248F) fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

PD-1 (Y223F, Y248F) (PDCD1 Human)

Use

Lentiviral

Tags

mEGFP

Mutation

PD-1 (Y223F, Y248F)

Promoter

SFFV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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plenti-puro-GSDMD WT

Plasmid

#249728

Purpose

Express GSDMD WT in mammalian cells with lentiviurs

Depositor

Hao Wu

Article

Du et al Nature. 2024 Jun;630(8016):437-446. doi:

Insert

gasdermin D (GSDMD Human)

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-APOBEC1-UdgX-NG-nCas9

Plasmid

#163562

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-APOBEC1-UdgX-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCherry.90 alpha

Plasmid

#108222

Purpose

Mammalian expression vector for mCherry fusion to human Hsp90 alpha

Depositor

Didier Picard

Article

Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

Insert

mCherry-Hsp90a (HSP90AA1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pOTTC374 - pAAV EF1a DIO iRFP

Plasmid

#47626

Purpose

An AAV vector that expresses iRFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Infrared fluorescent protein

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti GW V5 Eco/Dam hum LaminB1

Plasmid

#182671

Purpose

Eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin B1. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin B1.

Depositor

Bas van Steensel

Article

Guelen et al Nature. 2008 Jun 12;453(7197):948-51. do

Insert

Lamin B1 (LMNB1 Human)

Use

Lentiviral

Tags

E. Coli Dam and V5

Expression

Mammalian

Promoter

HSP

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CBA-hGBA1-cm-hGBA1-hGHpA

Plasmid

#218795

Purpose

AAV reporter genome plasmid

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

CMV-CBA-hGBA1-cm-hGBA1-hGHpA

Use

AAV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7B2Jnk2a2

Plasmid

#19727

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

pCDNA3 FlagMKK7B2Jnk2a2 (MAPK9, Map2k7 Human, Mouse)

Tags

Flag

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pSMALB-ATF4.5

Plasmid

#155032

Purpose

Fluorescent reporter for ATF4 translation

Depositor

John Dick, Peter van Galen

Article

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-TCOF1-repair-template

Plasmid

#237632

Purpose

For knock-in msfGFP to TCOF1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TCOF1 (TCOF1 Human)

Use

CRISPR

Tags

msfGFP

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSMALB-ATF4.14

Plasmid

#155034

Purpose

Fluorescent reporter for ATF4 translation, Positive Control

Depositor

John Dick, Peter van Galen

Article

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

uORF1 and uORF2 start codons mutated

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7B2Jnk1a1

Plasmid

#19726

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

MKK7B2Jnk1a1 (Map2k7, MAPK8 Human, Mouse)

Tags

Flag

Expression

Mammalian

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-MOSPD2 WT

Plasmid

#186472

Purpose

Expression of human MOSPD2 fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NPM1

Plasmid

#237667

Purpose

For overexpression of mEGFP-NPM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1 (NPM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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