We narrowed to 11,847 results for: NSI
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Plasmid#78198PurposeExpression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A197C)
ExpressionBacterialMutationA197C (please see depositors comment below)PromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-3XFLAG-renilla-luciferase-beta-globin-control
Plasmid#184393PurposeTransient transfection plasmid expressing Renilla-luciferase-beta-globin fusion proteinDepositorInsertRenilla luciferase
Tags3X FLAGExpressionMammalianPromoterCMVAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-G-GECO1.1
Plasmid#32445PurposeMammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)DepositorInsertG-GECO1.1
ExpressionMammalianMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…PromoterCMVAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRK5R 99xGly
Plasmid#211356PurposeTransient expression of 99xGly-EGFP-3xHADepositorInsert99xGly-EGFP-3xHA
TagsEGFP-3xHAExpressionMammalianPromoterCMVAvailable SinceOct. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRK5R 15xGly
Plasmid#211355PurposeTransient expression of 15xGly-EGFP-3xHADepositorInsert15xGly-EGFP-3xHA
TagsEGFP-3xHAExpressionMammalianPromoterCMVAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
RFP-PASS
Plasmid#193971PurposeMonitoring the production of phosphatidic acid with RFP-PASS (phosphatidic acid biosensor with superior sensitivity)DepositorInsertPA biosenor
ExpressionMammalianPromoterCMVAvailable SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
ExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57-GFP-2A-MCS
Plasmid#71783PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…ExpressionMammalianAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXJ-Myc-IRF1
Plasmid#232641Purposetransient expresses IRF1 in mammalian cellsDepositorAvailable SinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
lenti-mito-mTagBFP2
Plasmid#216130PurposeLentiviral vector for expressing mTagBFP2 localizing to mitochondria.DepositorInsertEF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
UseLentiviralExpressionMammalianAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
XZ375 EF1a-IL2Rbsp-3xFLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export
Plasmid#233386PurposeOptimized IL-2-sensing eLIDAR with IL-2 beta chain ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequenceDepositorInsertIL2Rb(ecto+TM)-ADAR2dd
TagsFLAGExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (Blast)
Plasmid#80921PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…ExpressionMammalianPromoterTREAvailable SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-N346A/C348A
Plasmid#127411PurposeDouble mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethersDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialMutationN346A-C348APromoteraraBADAvailable SinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAH-POLY-eRF1(E55D)
Plasmid#157925PurposeEncodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cellsDepositorInsertPOLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
ExpressionMammalianPromoterCMVAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_sfGPF 150TAG
Plasmid#154765Purposeplasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAGPromoterEF1Available SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cyto-roGFP
Plasmid#49435PurposeExpresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cellsDepositorInsertroGFP2
UseAdenoviralExpressionMammalianMutationC48S, Q80R, S147C, and Q204C in Clontech's G…PromoterCMVAvailable SinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
DD-DamV133A-LMNB1-IRES2-mCherry
Plasmid#159599PurposeShield-1 inducible transient mammalian expression of Dam-LMNB1 for DamID, containing the V133A mutant of Dam.DepositorInsertDD-DamV133A-LMNB1-IRES2-mCherry
ExpressionMammalianMutationDam V133A mutationPromoterCMVAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_mScarlet_CLASP_RGS2membrane
Plasmid#133086PurposePlasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to blDepositorInsertmScarlet-CLASP
ExpressionBacterial and YeastAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only