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pRK5M-TIMP3

Plasmid

#31715

Depositor

Rik Derynck

Article

Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

Insert

TIMP3 (TIMP3 Human)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RNF168

Plasmid

#133976

Purpose

mammalian expression vector of Flag tagged wild type RNF168

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGreenPuro-shRNA-Stx3S-C4

Plasmid

#99742

Purpose

3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

Depositor

Thomas Weimbs

Article

Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

Insert

shRNA against Stx3S

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FLCN

Plasmid

#72290

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pMAL-BRT17

Plasmid

#45358

Depositor

Scott Banta

Article

Shur et al Biotechniques. 2013 Apr;54(4):197-206. d

Insert

17 Repeat Beta Roll Tag

Tags

Maltose Binding Protein (MBP)

Expression

Bacterial

Promoter

Ptac

Available Since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

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pBAD/HisD-LSSmKate2

Plasmid

#31909

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSSmKate2

Tags

HIS

Expression

Bacterial

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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G608G lamin A minigene

Plasmid

#20292

Depositor

Tom Misteli

Article

Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

Insert

lamin A (LMNA Human)

Tags

GFP

Expression

Mammalian

Mutation

G606G:GGC>GGT Mutation causes aberrant spli…

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only
pET28a-MH6-uvsY

Plasmid

#163914

Purpose

Expresses uvsY for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsY (uvsY Enterobacteria phage T4)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

Plasmid

#180273

Purpose

Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-FLuc

Plasmid

#170575

Purpose

Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay.

Depositor

David Nemazee

Article

Rogers et al Science. 2020 Aug 21;369(6506):956-963.

Insert

Luc

Use

Luciferase and Retroviral

Tags

NA

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

Plasmid

#113898

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiblast-Paxillin-mTurqoise

Plasmid

#74206

Purpose

Lentiviral expression of Paxillin-mTurqoise

Depositor

Jayanta Debnath

Article

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

Paxillin

Use

Lentiviral

Tags

mTurqoise

Expression

Mammalian

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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NPY2R-Tango

Plasmid

#66454

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

NPY2R (NPY2R Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
GPR161-Tango

Plasmid

#66334

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR161 (GPR161 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
VV063: 1xCox8 - superecliptic pHluorin in fck

Plasmid

#58500

Purpose

Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - Superecliptic pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt3

Plasmid

#35909

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3 (WNT3 Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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TAL2472

Plasmid

#36853

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

TP53-TALEN_Right (Tp53 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2348

Plasmid

#36768

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

TP53-TALEN_Left (Tp53 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pSADdeltaG-BFP

Plasmid

#32639

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

BFP

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pBS human IRS-1

Plasmid

#11359

Depositor

Ronald Kahn

Article

Araki et al Diabetes. 1993 Jul . 42(7):1041-54.

Insert

IRS-1 (IRS1 Human)

Expression

Bacterial

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only

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