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pcDNA3-ALK6 wt

Plasmid

#80882

Purpose

mammalian expression of ALK6 WT

Depositor

Aristidis Moustakas

Publication

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK6 (Bmpr1b Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pMK278 (mClover-Hygro)

Plasmid

#72794

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Publication

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Hygro

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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hSERT-N1-pEYFP

Plasmid

#70105

Purpose

mammalian expression of hSERT with C-terminal YFP fusion

Depositor

Harald Sitte

Publication

Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

Insert

SERT (SLC6A4 Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pICSL11056

Plasmid

#68258

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Mouse Alpha2 delta-2 HA pMT2

Plasmid

#58731

Purpose

expression of HA tagged mouse Alpha2 delta-2 in mamalian cells

Depositor

Annette Dolphin

Publication

Davies et al J Neurosci. 2006 Aug 23;26(34):8748-57.

Insert

alpha2 delta-2 (Cacna2d2 Mouse)

Expression

Mammalian

Promoter

SV40

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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psicheck2 PTEN 3'UTR

Plasmid

#50936

Purpose

reporter for PTEN RNAi

Depositor

Pier Pandolfi

Publication

Tay et al Cell. 2011 Oct 14;147(2):344-57. doi: 10

Insert

PTEN 3'UTR (PTEN Human)

Use

RNAi

Tags

Renilla luciferase

Expression

Mammalian

Promoter

T7

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pRSV-CaMKII(1-290)

Plasmid

#45065

Purpose

Expression of constit active CaMKII

Depositor

Richard Maurer

Publication

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

CaM Kinase II (Camk2a Rat)

Expression

Mammalian

Mutation

truncated at amino acid 290

Promoter

RSV

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Integrin beta1-tev-BHCys

Plasmid

#27314

Depositor

Timothy Springer

Publication

Takagi et al Nat Struct Biol. 2001 May . 8(5):412-6.

Insert

integrin beta1 (ITGB1 Human)

Expression

Mammalian

Mutation

integrin beta1(1-708)+GG+TEV+GGKN+BHCys

Available since

April 8, 2011

Availability

Academic Institutions and Nonprofits only
2XMyc-LRRK2-Kinase

Plasmid

#25071

Depositor

Mark Cookson

Publication

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the kinase domain aa 1863-2218

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA5/FRT/TO GFP HSPA1L

Plasmid

#19484

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA1L (HSPA1L Human)

Tags

GFP

Expression

Mammalian

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mNP-Luc (PPARdelta promoter)

Plasmid

#16532

Depositor

Bert Vogelstein

Publication

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

PPAR delta promoter NP (PPARD Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant binding sites

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
PIK3CA-WT (pFastBac)

Plasmid

#16644

Depositor

Bert Vogelstein

Publication

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA (PIK3CA Human)

Use

Baculoviral

Tags

His

Expression

Mammalian

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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LB124 (Bcl-2 from -1287 to -3934)

Plasmid

#15240

Depositor

Linda Boxer

Publication

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -3934 with P1

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag-NLS-Smad2

Plasmid

#14931

Depositor

Joan Massague

Publication

Lo et al Nat Cell Biol. 1999 Dec . 1(8):472-8.

Insert

NLS-Smad2 (SMAD2 Human)

Tags

Flag

Expression

Mammalian

Mutation

full-length Smad2 is fused to a nuclear-localizat…

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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CS2 Flag-Smad3 (EPSM)

Plasmid

#14963

Depositor

Joan Massague

Publication

Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Insert

Smad3 (EPSM) (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T178V, S203A, S207A, S212A

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pcDNA3.1 FLIPE-1m

Plasmid

#13544

Depositor

Wolf Frommer

Publication

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP2B

Plasmid

#115331

Purpose

expresses mCherry-CHMP2B in mammalian cells

Depositor

Sanford Simon

Publication

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2B (CHMP2B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

silent mutation in position 4205 C to T

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5-Flag-Med25

Plasmid

#64771

Purpose

Mammalian protein expression of flag-tagged Med25

Depositor

Joan Conaway, Ronald Conaway

Publication

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25 (MED25 Human)

Tags

Flag

Expression

Mammalian

Promoter

pCMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pBAD/HisB-Medium-FT

Plasmid

#31917

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Medium-FT

Tags

His

Expression

Bacterial

Mutation

M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

Promoter

araB

Available since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

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3xFlag-eGFP-Flag-SETX

Plasmid

#218838

Purpose

Mammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.

Depositor

Albert La Spada

Publication

Bennett et al Heliyon. 2020 Jun 13;6(6):e04165. doi: 1

Insert

Senataxin (SETX Human)

Tags

Flag and eGFP

Expression

Mammalian

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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