We narrowed to 46,691 results for: cha;
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Plasmid#167898PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30b jGCaMP8f F366H
Plasmid#204139PurposeProduction of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coliDepositorInsertjGCaMP8f F366H
TagsN-terminal His tagExpressionBacterialMutationPhenylalanine 366 changed to histidinePromoterT7Available SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-31-PGK-GFP-Blast
Plasmid#31515DepositorAvailable SinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]
Plasmid#26569DepositorInsertcalcium channel alpha-1B subunit (Cacna1b Rat)
Tags6XHis and V5ExpressionMammalianMutationDeletion of exons a10, 18a, and 24a. Contains …Available SinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
EYFP Cav2.2 pRK5
Plasmid#206099Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal EYFP tag and an exofacial double HA tag in domain IIDepositorAvailable SinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-mCD59-T2A-GFP
Plasmid#205451Purposelentiviral plasmid for expression of mouse CD59DepositorAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB122
Plasmid#68576PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsModified EAR motif plant-specific repression doma…ExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2e[a10, 18a, Δ24a, 31a, 37b, 46]
Plasmid#26568DepositorInsertcalcium channel alpha-1B subunit (Cacna1b Rat)
Tags6XHis and V5ExpressionMammalianMutationcontains exons a10, 18a, 31a, 37b, and 46. Del…Available SinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNH13 (pmyo-2::QuasAr::mOrange)
Plasmid#130273PurposeExpresses the eFRET-based voltage sensor QuasAr::mOrange in the pharynx of C. elegans.DepositorInsertQuasAr::mOrange
TagsmOrangeExpressionWormAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-EPA-DNNNS-DQNRT
Plasmid#128404PurposePeriplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coliDepositorInsertExotoxin A (toxA P. aeruginosa)
Tags6xHis tag and DsbA signal sequence for periplasmi…ExpressionBacterialMutation242DNNNS246 and 384DQNRT388 engineered glycosylat…PromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-A'Q-mito
Plasmid#158763PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
ExpressionMammalianMutationFlexible linkers between the protease and msGFP a…Available SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN061
Plasmid#101725PurposeSingle copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsS. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastPromoterHeterologous TEF1 promoter from A. gossypii. and …Available SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pECE-M2-PAK2 S20A
Plasmid#31681DepositorInsertp21-activated protein kinase 2 S20A (PAK2 Human)
TagsM2ExpressionMammalianMutationchanged Serine 20 to AlanineAvailable SinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2e[Δa10, Δ18a, 24a, Δ31a, 37b, 46]
Plasmid#26571DepositorInsertcalcium channel alpha-1B subunit (Cacna1b Rat)
Tags6XHis and V5ExpressionMammalianMutationDeletion of exons a10, 18a, and 31a Contains e…Available SinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PIWI(aa478-860)
Plasmid#21543DepositorAvailable SinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
p164 Dync1i1.B
Plasmid#26435DepositorInsertcytoplasmic dynein 1 intermediate chain 1 isoform B (Dync1i1 Mouse)
UseSubcloning and sequencingMutationN/AAvailable SinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mEos3.2-A69T
Plasmid#98571Purposephotoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coliDepositorInsertmEos3.2-A69T
Tags7xHisExpressionBacterialPromoterT7Available SinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
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N-BLa 1.1
Plasmid#88994PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.1 fusion protein
TagsFlagExpressionBacterialPromoteraraBADAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSK267
Plasmid#129272Purpose"ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed proteinDepositorInsertNLS-VP16-ZF 43-8-NES
Tags6xHAExpressionYeastPromoterSUP35Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only