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pUPD2 TNos:Luc:5'UTR (GB1499)

Plasmid

#160580

Purpose

Reversed sequences of the NOS terminator, Firefly Luciferase CDS and 5' UTR of 35S promoter

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

Luc

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 PhiC31 integrase (GB1561)

Plasmid

#160586

Purpose

Streptomyces phage PhiC31 integrase, adapted for C-terminal fusions

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 linker-RDF (GB2892)

Plasmid

#160604

Purpose

RDF sequence for C-terminal fusion with PhiC31 recombinase, together with a 18-nt linker sequence

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

RDF

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Fah_S

Plasmid

#163086

Purpose

Swingshift mutant FAH in mammalian cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Inserts

Fah_S
Fah_S

Tags

Myc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Donor-PIGAshort

Plasmid

#153547

Purpose

Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

PIGA (a 603-bp fragment from intron 5 and exon 6)

Use

Donor plasmid

Tags

N/A

Mutation

No mutation

Promoter

No promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-5U6-sgRNAs-hsyn-EGFP

Plasmid

#112213

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

sgRNAs

Use

AAV

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPV-1.8k-Venus-WPRE

Plasmid

#156398

Purpose

Expresses Venus under PV gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfSP-0.8k-Venus-WPRE

Plasmid

#156397

Purpose

Expresses Venus under SP gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfSP-1.7k-Venus-WPRE

Plasmid

#156396

Purpose

Expresses Venus under SP gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfSERT-0.5k-Venus-WPRE

Plasmid

#156395

Purpose

Expresses Venus under SERT gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfvAChT-1.1k-Venus-WPRE

Plasmid

#156390

Purpose

Expresses Venus under vAChT gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfCCK-3.9k-Venus-WPRE

Plasmid

#156391

Purpose

Expresses Venus under CCK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET15-His-∆NGlpG(C104A/M247C)

Plasmid

#155133

Purpose

Expresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15b

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

His

Expression

Bacterial

Mutation

C104A/M247C, insert codes for GlpG residues 87-276

Promoter

T7

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pET_3H1L_02.395

Plasmid

#155198

Purpose

Expresses 3H1L_02.395 in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3H1L_02.395

Tags

6x His Tag

Expression

Bacterial

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA1-NLS(SV40) (KAC478)

Plasmid

#133795

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized AcrIIA1

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-100-1-dendra2

Plasmid

#105438

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-100-1 Dendra2 (mir100-1 Zebrafish)

Use

Zebrafish

Tags

N/A

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-132-2-dendra2

Plasmid

#105439

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-132-2 Dendra2 (mir132-2 Zebrafish)

Use

Zebrafish

Tags

N/A

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-lyzC-miR-429a-dendra2

Plasmid

#105443

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

miR-429a Dendra2 (mir429 Zebrafish)

Use

Zebrafish

Tags

N/A

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pKI-cjPLP1e5-S253T

Plasmid

#129755

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

PLP1 (PLP1 C. jacchus (common marmost))

Use

Marmoset targeting

Mutation

S253T

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjPLP1e5-P216S

Plasmid

#129754

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

PLP1 (PLP1 C. jacchus (common marmost))

Use

Marmoset targeting

Mutation

P216S

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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