Addgene

We narrowed to 6,032 results for: WUS;

Showing: 1531 - 1560 of 6032 results

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CAG.MCAK-mCherry-eDHFR

Plasmid

#176204

Purpose

MCAK (179-583) recruitment responsible for targeted microtubule depolymerization using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

MCAK-mCherry-eDHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pEMY07AB-PRO

Plasmid

#127710

Purpose

Level 0 destination vector for a promoter with A - B scars. Clone in with BpiI

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pRSM41

Plasmid

#113940

Purpose

Constitutive bacterial expression of red fluorescent Ca2+ sensor REX-GECO1

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

REX-GECO1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (Guinea Pig 1-88)

Plasmid

#62059

Purpose

Expression of Guinea Pig p53 (residues 1-88) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53

Use

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-88

Promoter

T7

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
CS2-GFP-nopo

Plasmid

#47964

Purpose

Expression of Fly nopo

Depositor

Laura Lee

Article

Merkle et al Development. 2009 Feb;136(3):449-59. doi

Insert

no poles (nopo Fly)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pattB-synaptobrevin-13-QFBDAD-G4MD257-761-hsp70

Plasmid

#46122

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QFBDAD-G4MD257-761

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pattB-synaptobrevin-12-QFBDAD-G4MD50-761-hsp70

Plasmid

#46121

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QFBDAD-G4MD50-761

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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phtsResA

Plasmid

#25914

Depositor

Johann Deisenhofer

Article

Colbert et al Proc Natl Acad Sci U S A. 2006 Mar 21. 1

Insert

His-tagged soluble ResA (resA Bacillus subtilis)

Use

Tags

His

Expression

Bacterial

Mutation

Start at Serine 37 to remove n-terminal transmemb…

Promoter

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pEMY07AB-ORF

Plasmid

#127711

Purpose

Level 0 destination vector for a coding sequence with B - C scars. Clone in with BpiI

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pEMY07CD-TER

Plasmid

#127712

Purpose

Level 0 destination vector for a terminator with C - D scars. Clone in with BpiI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-5a

Plasmid

#127716

Purpose

Level 1 destination vector for a transcription unit, pathway position 1. Clone a transcription unit in with BsaI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pDSM-ab

Plasmid

#127717

Purpose

Level 1 destination vector for a transcription unit, pathway position 2. Clone a transcription unit in with BsaI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pDSM-bc

Plasmid

#127718

Purpose

Level 1 destination vector for a transcription unit, pathway position 3. Clone a transcription unit in with BsaI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-cd

Plasmid

#127719

Purpose

Level 1 destination vector for a transcription unit, pathway position 4. Clone a transcription unit in with BsaI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-de

Plasmid

#127720

Purpose

Level 1 destination vector for a transcription unit, pathway position 5. Clone a transcription unit in with BsaI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-ef

Plasmid

#127721

Purpose

Level 1 destination vector for a transcription unit, pathway position 6. Clone a transcription unit in with BsaI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-f3

Plasmid

#127722

Purpose

Level 1 destination vector for a transcription unit, pathway position 7. Clone a transcription unit in with BsaI.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pBoBi-hAP1-C-LAMP2 TM

Plasmid

#183645

Purpose

Lentiviral expression of a mutant of ATP6AP1 with its PEN2-interacting domain replaced with the transmembrane (TM) domain of LAMP2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

ATP6AP1 and LAMP2 (ATP6AP1, LAMP2 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

ATP6AP1 transmembrane domain replaced by the tran…

Promoter

CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Gpi1

Plasmid

#175934

Purpose

knockout mouse Gpi1

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Gpi1 gRNA (Gpi1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28c S2

Plasmid

#171920

Purpose

Express RNA-based (p)ppGpp sensor

Depositor

Mingxu You

Article

Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

Insert

RNA-based (p)ppGpp sensor

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_mCherry_P2A_Hygro

Plasmid

#135003

Purpose

Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-pmDH

Plasmid

#67897

Purpose

Expresses a fusion of mCherry with the membrane located DH domain (1-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-pmDH

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-MBP-TEV site-TadA8.20

Plasmid

#194702

Purpose

Expresses N-terminal MBP fused TadA8.20 in bacteria

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

TadA8.20

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

T7 promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet S2

Plasmid

#171921

Purpose

Express RNA-based (p)ppGpp sensor and a NirFP

Depositor

Mingxu You

Article

Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

Inserts

RNA-based (p)ppGpp sensor
NirFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Lifeact-tdTomato

Plasmid

#64048

Purpose

Fluorescent reporter for F-actin labeling in living cells

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Lifeact

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003-mi3

Plasmid

#159995

Purpose

Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyCatcher003-mi3

Use

Tags

C-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Frb-EGFP-HOTag6

Plasmid

#106919

Purpose

Express Frb-EGFP-HOTag6 fusion in mammalian cells.

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

Frb-EGFP-HOTag6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-SV40LT

Plasmid

#136616

Purpose

Dox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cells

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

SV40 large T antigen

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FKBP-EGFP-HOTag3

Plasmid

#106924

Purpose

Expresses FKBP-EGFP-HOTag3 in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

FKBP-EGFP-HOTag3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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G6PD/pRK5

Plasmid

#41521

Depositor

Xiaolu Yang

Article

Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

Insert

glucose-6-phosphate dehydrogenase (G6PD Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only

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