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Showing: 2041 - 2080 of 2761 results

  1. p15A-PB

    Plasmid
    #133886
    Purpose
    piggyBac ITR's in low copy number p15A backbone plasmid
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Piggybac
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133886-plasmid-map-sequence-id-274420

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  2. pRS416 GAL1pro-YFP

    Plasmid
    #224875
    Purpose
    Low copy plasmid with GAL1-driven YFP for C-terminal tagging (no start codon)
    Depositor
    Paul Herman
    Type
    Empty backbone
    Use
    Tags
    YFP
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    224875-plasmid-map-sequence-id-448177

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  3. 474 p5E-basprom

    Plasmid
    #31158
    Depositor
    Nathan Lawson
    Insert
    basal adenovirus E1b TATA element
    Use
    Entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2011
    Availability
    Academic Institutions and Nonprofits only
    31158-plasmid-map-sequence-id-37676

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  6. Pin1

    Plasmid
    #40773
    Depositor
    Dustin Maly
    Insert
    Pin1 (PIN1 Human)
    Use
    Tags
    6His and TEV
    Expression
    Bacterial
    Mutation
    Starting Met removed
    Promoter
    T7
    Available since
    Oct. 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    40773-plasmid-map-sequence-id-129516

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  7. pRAC01A

    Plasmid
    #193730
    Purpose
    Expresses deactivated-Cas9 (J23101) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion
    Depositor
    Martin Buck
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193730-plasmid-map-sequence-id-381916

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  8. pRAC07A

    Plasmid
    #193733
    Purpose
    Expresses deactivated-Cas9 (J23107) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion
    Depositor
    Martin Buck
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193733-plasmid-map-sequence-id-381918

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  9. pDLucBiDi

    Plasmid
    #219562
    Purpose
    Dual luciferase plasmid with Renilla luciferase constitutively expressed and firefly luciferase downstream of MCS without a start codon
    Depositor
    Pasha Baranov
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40 and T7
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    219562-plasmid-map-sequence-id-439245

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  10. pRS414-Syf1 817 Truncation

    Plasmid
    #234126
    Purpose
    Syf1 PCR truncation starting at 817 through the end of the gene
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Use
    Tags
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Promoter
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234126-plasmid-map-sequence-id-466534

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  11. pRS414-Syf1 634 Truncation

    Plasmid
    #234116
    Purpose
    Syf1 PCR truncation starting at 634 through the end of the gene
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Use
    Tags
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Promoter
    Available since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234116-plasmid-map-sequence-id-466457

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  12. pRS414-Syf1 778 Truncation

    Plasmid
    #234115
    Purpose
    Syf1 PCR truncation starting at 778 through the end of the gene
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Use
    Tags
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234115-plasmid-map-sequence-id-465993

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  13. pRAC10A

    Plasmid
    #193734
    Purpose
    Expresses deactivated-Cas9 (J23110) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion
    Depositor
    Martin Buck
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193734-plasmid-map-sequence-id-381905

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  14. pLenti-0.5Syn-Exon 1 of BNDF-D2GFP-WPRE

    Plasmid
    #233060
    Purpose
    To express D2GFP from a 0.5 bp synapsin promoter. The non-coding BDNF exon one is 5' to the BDNF start codon.
    Depositor
    Jonathan Ploski
    Insert
    D2GFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    233060-plasmid-map-sequence-id-472159

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  15. pSpy

    Plasmid
    #29588
    Depositor
    Marc Fiedler
    Insert
    Spy (spy E.coli BL21(DE3))
    Use
    Tags
    Expression
    Bacterial
    Mutation
    processed form of Spy starting at residue 24 (Rem…
    Promoter
    Available since
    April 28, 2011
    Availability
    Academic Institutions and Nonprofits only

  16. pCambia-dLB-kanR-GFP

    Plasmid
    #223177
    Purpose
    Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border
    Depositor
    Andreas Stuermer
    Type
    Empty backbone
    Use
    Ti plasmid for agrobacterium
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    223177-plasmid-map-sequence-id-443110

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  17. ORFtag_LambdaN-SD_Frame3

    Plasmid
    #220986
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame3
    Depositor
    Alexander Stark
    Insert
    lambdaN
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220986-plasmid-map-sequence-id-437120

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  18. ORFtag_LambdaN-SD_Frame1

    Plasmid
    #220984
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame1
    Depositor
    Alexander Stark
    Insert
    lambdaN
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    220984-plasmid-map-sequence-id-437511

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  19. ORFtag_LambdaN-SD_Frame2

    Plasmid
    #220985
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame2
    Depositor
    Alexander Stark
    Insert
    lambdaN
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    220985-plasmid-map-sequence-id-437496

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  20. HC080

    Plasmid
    #59545
    Purpose
    Expresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tag
    Depositor
    Daniel Klionsky
    Insert
    ATG13 (ATG13 Budding Yeast)
    Use
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    AvrII site created after start codon
    Promoter
    ATG13
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. pJK366

    Plasmid
    #72405
    Purpose
    Produces Acetobacter aceti 1023 Est02
    Depositor
    T. J. Kappock
    Insert
    peptidase S9
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal MVKNGS sequence, alternate …
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72405-plasmid-map-sequence-id-135520

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  22. pJK377

    Plasmid
    #72411
    Purpose
    Produces Acetobacter aceti 1023 Est05
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal MTFLYSICHIVMNIVSSTPHFNVWICF…
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72411-plasmid-map-sequence-id-135530

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  23. ORFtag_TetR-SD_Frame2

    Plasmid
    #220982
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame2
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220982-plasmid-map-sequence-id-437118

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  24. ORFtag_TetR-SD_Frame3

    Plasmid
    #220983
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame3
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220983-plasmid-map-sequence-id-437500

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  25. pNHA_OROV_NCP

    Plasmid
    #208937
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus nucleocapsid protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    Nucleocapsid
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted start codon so HA included at N-terminus
    Promoter
    CMV
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208937-plasmid-map-sequence-id-418963

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  26. pJK406

    Plasmid
    #72420
    Purpose
    Produces Acetobacter aceti 1023 Est05, C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    additional N-terminal MTFLYSICHIVMNIVSSTPHFNVWICF…
    Promoter
    T7
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72420-plasmid-map-sequence-id-135533

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  27. pJK267

    Plasmid
    #72432
    Purpose
    Acetobacter aceti 1023 gDNA
    Depositor
    T. J. Kappock
    Insert
    Acetobacter aceti 1023 gDNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    n/a
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  28. pJK368

    Plasmid
    #72407
    Purpose
    Produces Acetobacter aceti 1023 Est12
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioesterase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal 15 residues MSETYGTGQKEQHC,…
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72407-plasmid-map-sequence-id-135653

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  29. m2ap

    Plasmid
    #50051
    Purpose
    expresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cells
    Depositor
    Timothy Springer
    Insert
    M2AP
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    starts at aa47; N127R, S158A, S177N, N357S, N463S…
    Promoter
    CMV
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. ORFtag_TetR-SD_Frame1

    Plasmid
    #220981
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK promoter
    Available since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    220981-plasmid-map-sequence-id-437540

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  31. Mek2

    Plasmid
    #40776
    Depositor
    Dustin Maly
    Insert
    MKK2 (MAP2K2 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Starting Met removed
    Promoter
    CMV
    Available since
    Oct. 15, 2012
    Availability
    Academic Institutions and Nonprofits only

  32. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121176-plasmid-map-sequence-id-235001

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  33. Unc104_K560GFP

    Plasmid
    #129773
    Purpose
    Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    Unc104 (unc-104 Nematode)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 361, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129773-plasmid-map-sequence-id-263568

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  34. lentiCRISPRv2_PROX1_1+4

    Plasmid
    #121177
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121177-plasmid-map-sequence-id-237896

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  35. pJK404

    Plasmid
    #72417
    Purpose
    Produces Acetobacter aceti 1023 Est12, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    additional N-terminal 15 residues MSETYGTGQKEQHC,…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72417-plasmid-map-sequence-id-135656

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  36. Mek1

    Plasmid
    #40774
    Depositor
    Dustin Maly
    Insert
    MKK1 (MAP2K1 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Starting Met removed
    Promoter
    CMV
    Available since
    Oct. 15, 2012
    Availability
    Academic Institutions and Nonprofits only

  37. tol2-4xnr UAS-PA Rac1-mCherry

    Plasmid
    #41878
    Depositor
    Anna Huttenlocher
    Insert
    photoactivatable Rac1 (RAC1 Human)
    Use
    Zebrafish expression
    Tags
    6x His and mCherry
    Expression
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Promoter
    Available since
    Oct. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    41878-plasmid-map-sequence-id-56671

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  39. pENTR223 TMPRSS2, closed

    Plasmid
    #149721
    Purpose
    for Gateway cloning of TMPRSS2 into destination vector
    Depositor
    Anne-Claude Gingras
    Insert
    TMPRSS2 (TMPRSS2 Human)
    Use
    Entry vector
    Tags
    Expression
    Mutation
    wt, without start codon
    Promoter
    Available since
    June 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149721-plasmid-map-sequence-id-298609

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  40. pKAN43Ag8-GFP

    Plasmid
    #178182
    Purpose
    Plant binary vector expressing GFP by a enhanced 35S promoter
    Depositor
    Keiichi Shimizu
    Insert
    EGFP (eGFP )
    Use
    Tags
    Expression
    Plant
    Mutation
    The CAT is inserted after the start codon.
    Promoter
    The minimal 35S promoter from Cauliflower Mosaic …
    Available since
    Sept. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    178182-plasmid-map-sequence-id-446882

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Showing: 2041 - 2080 of 2761 results