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pcDNA-Wnt7A-V5

Plasmid

#35933

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7A (WNT7A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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MBD1v1 pcDNA3.1

Plasmid

#78140

Purpose

Mammalian expression of MBD1v1

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MBD1 (MBD1 Human)

Use

Tags

Expression

Mammalian

Mutation

v1 isoform

Promoter

CMV

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-)_CMVtrunc_877

Plasmid

#105828

Purpose

A modified pcDNA3.1- vector with reduced expression activity of a truncated CMV promoter

Depositor

Harald Janovjak

Article

Kainrath et al Angew Chem Int Ed Engl. 2017 Apr 10;56(1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt10B-V5

Plasmid

#35940

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10B (WNT10B Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Caspase 12

Plasmid

#35574

Depositor

Junying Yuan

Article

Nakagawa et al Nature. 2000 Jan 6. 403(6765):98-103.

Insert

Caspase 12 (Casp12 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2012

Availability

Academic Institutions and Nonprofits only
pcDNAIIIB HA JNK

Plasmid

#61630

Purpose

mammalian expression of JNK

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

JNK1 beta1 isoform (MAPK8 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-ΔN-RNMT

Plasmid

#112708

Purpose

Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Use

Tags

FLAG and HIV Rev nuclear export signal (NES)

Expression

Mammalian

Mutation

deleted amino acids 2-120, mutated active-site D2…

Promoter

CMV

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-KLF3

Plasmid

#49100

Purpose

Expresses KLF3 in mammalian cells

Depositor

Merlin Crossley

Article

Bell-Anderson et al Diabetes. 2013 Aug;62(8):2728-37. doi: 1

Insert

Krüppel-Like Factor 3(KLF3)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-PU.1-ETS

Plasmid

#124652

Purpose

Chimeric ETS domain

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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Lyn-OeNL_pcDNA3

Plasmid

#89528

Purpose

Orange color variant of bright luminescent protein, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

Lyn

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+):RGS4

Plasmid

#31157

Depositor

Henry Lester

Article

Doupnik et al Proc Natl Acad Sci U S A. 1997 Sep 16. 9

Insert

Regulator of G Protein Signaling 4 (Rgs4 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-BREP

Plasmid

#172337

Purpose

BRET-Based Bioluminescent Red Protein

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BREP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-deltaCKAR

Plasmid

#31526

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Delta-selective C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3 RL3Sc

Plasmid

#109365

Purpose

Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3 V5 mDispatched WT 2x HA

Plasmid

#126410

Purpose

Expression of V5 Dispatched WT HA in mammalian cell lines.

Depositor

Stacey Ogden

Article

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

m-Dispatched1 (Disp1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

V5 iserted between A106 and A107

Promoter

Available since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 NHE-RF

Plasmid

#11635

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

NHE-RF (NHERF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK5kin

Plasmid

#65245

Purpose

Expression of HA tagged ERK5 kinase domain in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5kin (MAPK7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 410-817

Promoter

CMV

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)_UGControl

Plasmid

#133875

Purpose

Expresses UGControl in mammalian cells

Depositor

Sumio Terada

Article

Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

Insert

UtrCH (UTRN Human)

Use

Tags

flexible linker - mEGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1YFP8

Plasmid

#41669

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Use

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; 8 C-terminal-m…

Promoter

CMV

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 T7 DLC1

Plasmid

#24266

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC1 (DYNLL1, DLC1 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only