We narrowed to 6,524 results for: sas

1. pcFLAG-Grap2-WT

   Plasmid

   #74511

   Purpose

   Overexpression of Grap2 in mammalian cells

   Depositor

   Lars Rönnstrand

   Article

   Chougule et al Oncotarget. 2016 Feb 15. doi: 10.18632/o

   Insert

   Grap2 (GRAP2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pResQ shDna2'

   Plasmid

   #31952

   Depositor

   Sheila Stewart

   Article

   Duxin et al Mol Cell Biol. 2009 Aug;29(15):4274-82.

   Insert

   shRNA against hDna2 (DNA2 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pCfB2802

   Plasmid

   #63655

   Purpose

   pCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Expression

   Yeast

   Promoter

   TEF1 promoter

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB1A3 EcoRI Methylase-AmilCP

   Plasmid

   #85166

   Purpose

   For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.

   Depositor

   Robin Dowell

   Article

   Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

   Insert

   EcoRI Methylase-AmilCP
   

   Tags

   AmilCP

   Expression

   Bacterial

   Promoter

   J23100 promoter

   Available Since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCfB2990

   Plasmid

   #63645

   Purpose

   pCfB2990 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Yeast

   Promoter

   none

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2

   Plasmid

   #69225

   Purpose

   Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS2 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEos2-FilaminA-C-14

   Plasmid

   #57378

   Purpose

   Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   FilaminA (FLNA Human)
   

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   D2626H

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pCLC3sFlag

   Plasmid

   #52422

   Purpose

   mammalian expression of flag tagged rat CLC3 short transcript

   Depositor

   Steven Weinman

   Article

   Shimada et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   CLC3 (Clcn3 Rat)
   

   Tags

   flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. EC71139

   Plasmid

   #154062

   Purpose

   Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   pZmUbi
   

   Use

   Synthetic Biology

   Mutation

   All BsaI, Esp3I and BpiI restriction sites were r…

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. hC73A-pEGFP_N1

    Plasmid

    #111533

    Purpose

    Expresses human KCNB1 C73A in mammalian cells for imaging

    Depositor

    Federico Sesti

    Article

    Yu et al Cell Death Dis. 2017 Apr 6;8(4):e2737. d

    Insert

    human C73A-Kv2.1 (KCNB1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C73A

    Promoter

    CMV

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. SauCas9KKH CCR5-nicking sgRNA

    Plasmid

    #169863

    Purpose

    SauCas9KKH nicking sgRNA for CCR5

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SauCas9KKH CCR5-nicking sgRNA
    

    Expression

    Mammalian

    Promoter

    human U6 promoter

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCfB2800

    Plasmid

    #63657

    Purpose

    pCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    TEF1 promoter

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3

    Plasmid

    #69226

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS3 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pML2(−)WF

    Plasmid

    #108337

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268

    Plasmid

    #69224

    Purpose

    Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS and Zif268

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSMP-MBD1_1

    Plasmid

    #36365

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    MBD1 (MBD1 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pSMP-MBD1_2

    Plasmid

    #36366

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    MBD1 (MBD1 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pRPB1-CTD14

    Plasmid

    #112822

    Purpose

    pSMF2 with 14 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB2799

    Plasmid

    #63658

    Purpose

    pCfB2799 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    TEF1 promoter

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB2992

    Plasmid

    #63642

    Purpose

    pCfB2992 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    none

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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