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pEGFP-C1-NuMA

Plasmid

#81029

Purpose

Expresses human NuMA with N-terminal EGFP tag in mammalian cells

Depositor

Michael Mancini

Insert

Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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SlugCas9-HF

Plasmid

#163796

Purpose

Expresses highly specific SlugCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Mutation

SlugCas9 (R247A, N415A, T421A, R656A)

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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Notch3 intracellular domain-pcw107-V5

Plasmid

#64623

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH3 (NOTCH3 Human)

Use

Lentiviral

Tags

V5

Mutation

intracellular domain *

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-SFNp-mRFP1

Plasmid

#238473

Purpose

Expresses higher mRFP1 fluorescence when NFkB is more active. Cloned into pLenti backbone

Depositor

Christopher Nelson

Article

Ivy et al BMC Methods. 2025;2(1):8. doi: 10.1186/s

Insert

SFNp-mRFP1

Promoter

SFNp

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6e

Plasmid

#207855

Purpose

Mammalian expression of PE6e prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6e

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, K918A, K775R)

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHyo245

Plasmid

#173147

Purpose

Expresses pheS_A294G by dual T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

Insert

pheS_A294G

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-G-GECO1.2

Plasmid

#32446

Purpose

Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.2

Expression

Mammalian

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pBK1340-LV-DUAL-dGFP-NanoLuc-Puro

Plasmid

#223160

Purpose

Expression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-life

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

dGFP-P2A-NanoLuc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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SepRS(2)/pSertRNA(B4)/EF-Sep

Plasmid

#173897

Purpose

The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

Depositor

Jason W Chin

Article

Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

Inserts

SepRS(2)
pSertRNA
EF-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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HA-AMOT p130

Plasmid

#32821

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

2xHA

Expression

Mammalian

Mutation

E5D

Promoter

CMV

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only

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