19,834 results

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Mito-mGreenLantern

Plasmid

#164461

Purpose

Localization of mGreenLantern fluorescent protein to mitochondria

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

Human COX8a first 29 amino acids

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA

Plasmid

#177576

Purpose

Expresses SOUL in neuronal cell types

Depositor

Guoping Feng

Article

Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

Insert

SOUL

Use

AAV

Tags

tdTomato

Promoter

hSyn

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-miR21

Plasmid

#21114

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

miR-21 (MIR21 Human)

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pcdna3.1 hciap2mut

Plasmid

#8339

Depositor

Jon Ashwell

Article

Li et al Nature 2002 Mar 21;416(6878):345-7.

Insert

ciap2mut (BIRC3 Human)

Tags

myc

Expression

Mammalian

Mutation

H574A

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pLXIN-Luc

Plasmid

#60683

Purpose

stable expresses luciferase in mammalian cells by retrovirus system

Depositor

Alice Wong

Insert

Firefly Luciferase

Use

Retroviral

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only
human EB1 fused to monomeric RFP

Plasmid

#39323

Depositor

Tim Mitchison, Jennifer Tirnauer

Inserts

EB1 (MAPRE1 Human)
mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pEF-XMAS-1xStop

Plasmid

#164411

Purpose

Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

mCherry

Use

CRISPR and Synthetic Biology

Tags

T2A-GFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pET30-HPF1-His-Sumo-Flag

Plasmid

#111577

Purpose

Expresses full length HPF1 in E. coli

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

HPF1 (HPF1 Human)

Tags

6xHis-Sumo-FLAG

Expression

Bacterial

Promoter

T7

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF2-O2S

Plasmid

#21162

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4, Sox2 (SOX2, POU5F1 Human)

Use

Lentiviral; Reprogramming

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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mApple-Sec61b-C1

Plasmid

#90993

Purpose

Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mApple-Sec61b (SEC61B Human, Synthetic)

Tags

mApple (red fluorescence)

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyTagMBP

Plasmid

#35050

Depositor

Mark Howarth

Article

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyTagMBP

Tags

His6

Expression

Bacterial

Mutation

Simple peptide tag added to the N-terminus of MBP…

Promoter

T7 promoter

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA

Plasmid

#20299

Purpose

Cre-activated AAV expression of mCherry

Depositor

Karl Deisseroth

Has Service

AAV9

Insert

mCherry

Use

AAV

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pMDIAI

Plasmid

#51655

Purpose

E coli genetic modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

apramycin antibiotic resistance gene

Use

Genetic modification

Promoter

unknown

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-hSyn::mCherry.3xFLAG-WPRE

Plasmid

#120392

Purpose

pAAV plasmid with BspQI sites for insertion of target specific shRNAs in frame of the murine U6 promoter and co expressing mCherry.3XFLAG under the hSyn promoter

Depositor

Florian Freudenberg

Article

Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

Type

Empty backbone

Use

AAV

Tags

mCherry.3XFLAG

Promoter

U6

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-SpdCas9-VP64

Plasmid

#115794

Purpose

Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

VP64

Use

AAV

Tags

3XFLAG and dCas9-VP64

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pNR220

Plasmid

#79541

Purpose

Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Inserts

TP901
BxbI
A118

Tags

His tag and LAA degradation tag

Expression

Bacterial

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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IL6-GFP

Plasmid

#28088

Depositor

Jennifer Stow

Article

Manderson et al J Cell Biol. 2007 Jul 2. 178(1):57-69.

Insert

IL6 (Il6 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

March 17, 2011

Availability

Academic Institutions and Nonprofits only

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pCMVMagFRET-1

Plasmid

#50742

Purpose

Expresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.

Depositor

Maarten Merkx

Article

Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

Insert

gene encoding for MagFRET-1

Expression

Mammalian

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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TOM20MTS-mCherry-EGFP-Tet-On

Plasmid

#109016

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged TOM20MTS (mitochondria

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

TOM20MTS (MTS: mitochondria targeting signal) (TOMM20 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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PCS4 3XFLAG-PPARgamma1

Plasmid

#78769

Purpose

To overexpress PPARgamma1 in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPAR_1 (Pparg Mouse)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV IE94

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only

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