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plenti.CMV.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#236407

Purpose

Expresses cytosolic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pICH-nuc-roGFP2-synORP1

Plasmid

#191759

Purpose

Ti plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

nuc-roGFP2-synORP1

Use

Binary agrobacterium ti plasmid

Expression

Plant

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBS hShh (CT#401)

Plasmid

#13996

Depositor

Cliff Tabin

Article

Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

Insert

Shh (SHH Human)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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MTK3_038

Plasmid

#123752

Purpose

Encodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

R-FlincA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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FUGW-d2GFP-ZEB1 3'UTR

Plasmid

#79601

Purpose

FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

d2GFP-Zeb1 3'UTR

Use

Lentiviral

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1ab-Prkd1

Plasmid

#139314

Purpose

Diacylglycerol biosensor

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry:Mus musculus Prkd1(138-343) (Prkd1 Mouse)

Expression

Mammalian

Mutation

T208A, N313D

Available Since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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MAC_N_TOM22

Plasmid

#111697

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

TOM22 (TOMM22 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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mtsCOXVIIIx2-B-gTEMP/pcDNA3

Plasmid

#199806

Purpose

Fluorescent temperature indicator B-gTEMP fused with mitochondrial targeting sequence

Depositor

Takeharu Nagai

Article

Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

Insert

B-gTEMP

Tags

mtsCOXVIIIx2

Expression

Mammalian

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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Red Glifon 3000

Plasmid

#163116

Purpose

Red fluorescent protein-based glucose sensor for mammalian cells with lower affinity

Depositor

Takashi Tsuboi

Article

Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

Insert

Red Glifon 3000

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)

Plasmid

#162230

Purpose

Lentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG17 GFP nanobody SynNotch tTA

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1-alpha-V

Plasmid

#27290

Depositor

Timothy Springer

Article

Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Insert

integrin alpha V (ITGAV Human)

Expression

Mammalian

Mutation

Isoform 2

Available Since

March 8, 2011

Availability

Academic Institutions and Nonprofits only

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DYSF-3HA

Plasmid

#29767

Depositor

Steven Vogel

Article

Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

Insert

DYSF (DYSF Human)

Tags

3xHA

Expression

Bacterial and Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pEG BacMam C term GFP His8

Plasmid

#160681

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

His8 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCMU-MTUBr

Plasmid

#61196

Purpose

Fluorescent reporter for microtubules mCherry-MAP4-MBD expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MAP-MBD

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pC1-mitoRexYFP

Plasmid

#60246

Purpose

Mitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

Depositor

Vsevolod Belousov

Article

Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

Insert

mitoRexYFP

Tags

Mitochondrial targeting signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoB-NAD

Plasmid

#193824

Purpose

CMV overexpression of ChemoB-NAD in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB-NAD

Expression

Mammalian

Mutation

EBFP2:N39Y, A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMM114

Plasmid

#127211

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, IPT, sgRNA, Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-2

Plasmid

#204200

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Tags

T7x3

Expression

Mammalian

Mutation

silent mutations

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-{CMV-CuO}>{MCS}:T2A:mCherry

Plasmid

#236249

Purpose

Puromycin selected lentiviral vector with MCS for cumate-inducible gene, for use with dual-inducible CymR-CuO system

Depositor

Genevieve Kendall

Article

Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-CuO

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pX458-ECFP

Plasmid

#112220

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

ECFP

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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