We narrowed to 22,939 results for: REP

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EF1a_KLF15_P2A_Hygro_Barcode

Plasmid

#120501

Purpose

Barcoded lentiviral vector to express KLF15 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF15 (KLF15 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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[GG][Pin]

Plasmid

#97191

Purpose

Pin nuclease domain with Pum cloning site

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Insert

Pin domain

Use

Synthetic Biology

Tags

Pum cloning site

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-A114-N40

Plasmid

#50561

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-A114-N40

Use

Cloning vector

Promoter

lac

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSMP-EED_2

Plasmid

#36386

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EED (EED Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pCI-OR2W1

Plasmid

#21686

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

Homo sapiens olfactory receptor, family 2, subfamily W, member 1 (OR2W1 Human)

Tags

Rho

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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Mammalian_SpCas9_Target-AID3S

Plasmid

#188649

Purpose

Expresses SpCas9_Target-AID3S in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID3S-UGI

Tags

UGI

Expression

Mammalian

Mutation

D10A for SpCas9

Promoter

pCMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRU53

Plasmid

#167679

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::spCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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sg2.0 (PP7) Locus#2

Plasmid

#101155

Purpose

gRNA with two PCP binding sites

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

Locus #2(tandem repeat)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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8522-M01-663

Plasmid

#225660

Purpose

Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCopGFP

Use

Lentiviral

Promoter

SORE6-mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3

Plasmid

#136364

Purpose

Expresses epitope-tagged human TET3 WT in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Tags

2xStrep-tagII, FLAG, and HA

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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TRMPV-ns

Plasmid

#27991

Purpose

Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.

Depositor

Scott Lowe

Article

Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

Insert

TRMPV-ns (shRen)

Use

RNAi and Retroviral; Fluorescent reporters

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pSCARS1

Plasmid

#174868

Purpose

Contains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1

Depositor

Zengyi Shao

Article

Lopez et al Appl Microbiol Biotechnol. 2021 Aug 6. p

Insert

ARS1

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAMS366-4xCDRE-GFP

Plasmid

#138657

Purpose

stable GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiae

Depositor

Sabrina Büttner

Article

Diessl et al Microbial Cell, 2020: in press

Insert

4xCDRE-CYC1(PM)-yeGFP

Use

Yeast reporter plasmid

Promoter

CYC1

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCF160_VSV-G

Plasmid

#225963

Purpose

CMV-Intron-VSVG (env protein). Expresses VSV-G env for VLP production.

Depositor

Christof Fellmann

Article

Ramadoss et al Nat Commun. 2025 Nov 17;16(1):9883. doi:

Insert

CMV-Intron-VSVG (env protein)

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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L1_lacZgRNA-Ck3

Plasmid

#136136

Purpose

L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF5_P2A_Hygro_Barcode

Plasmid

#120491

Purpose

Barcoded lentiviral vector to express KLF5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF5 (KLF5 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_FOXA3_P2A_Hygro_Barcode

Plasmid

#120440

Purpose

Barcoded lentiviral vector to express FOXA3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FOXA3 (FOXA3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PB-tight-DDdCas9GFP-IRES-Neo

Plasmid

#102907

Purpose

PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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CAGhP14

Plasmid

#84362

Purpose

express FLAG-HA tagged human PRDM14

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

silent mutations at coding silent 1068CtoT, 1629T…

Promoter

CAG

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pSMP-SETDB1_2

Plasmid

#36348

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SETDB1 (SETDB1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only