165,688 results

1. shh10

   Plasmid

   #64867

   Purpose

   This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

   Depositor

   John Flannery, David Schaffer

   Article

   Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

   Insert

   shh10 cap
   

   Use

   AAV

   Mutation

   I319V, N451D, D532N

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. zWnt5b

   Plasmid

   #21282

   Depositor

   Randall Moon

   Article

   Stoick-Cooper et al Development. 2007 Feb . 134(3):479-89.

   Insert

   Wnt5b (wnt5b Zebrafish)
   

   Available Since

   May 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. p5155

   Plasmid

   #175391

   Purpose

   Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)

   Depositor

   Michael Wiles

   Article

   Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

   Type

   Empty backbone

   Use

   Mouse Targeting; Cloning vector, to be used to de…

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRE:GFP

   Plasmid

   #163036

   Purpose

   FLiCRE reporter gene for neuronal expression

   Depositor

   Alice Ting

   Article

   Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

   Insert

   GFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX303

   Plasmid

   #25897

   Purpose

   3rd generation lentiviral Gateway destination vector. Blasticidin selection.

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Expression

   Mammalian

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. MS2-p65-HSF1-P2A-mCherry

   Plasmid

   #184440

   Purpose

   MS2 coat protein fused with p65 and hsf1

   Depositor

   Rhys Allan

   Article

   Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

   Insert

   MS2-p65-hsf1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKI1.1R

   Plasmid

   #85808

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Plant

   Promoter

   AtRPS5A

   Available Since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLTS

   Plasmid

   #59386

   Purpose

   Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Type

   Empty backbone

   Use

   Genome engineering

   Expression

   Bacterial

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHM131

   Plasmid

   #225530

   Purpose

   Encodes destablized mCherry under IBRE4 promoter, and constitutive BFP

   Depositor

   Jared Toettcher

   Article

   McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

   Inserts

   mCherry
   BFP
   

   Expression

   Mammalian

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-LUC2CP/intron/ARE

    Plasmid

    #62858

    Purpose

    splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

    Depositor

    Gideon Dreyfuss

    Article

    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

    Insert

    Luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    insertion of chimeric intron at nueotide poclsiti…

    Promoter

    CMV

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV_TRET_hNgn2_UBC_Puro

    Plasmid

    #61474

    Purpose

    3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection marker

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Inserts

    Ngn2 (NEUROG2 Human)
    Puro
    

    Use

    Lentiviral

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. HsTPC2-EYFP

    Plasmid

    #135194

    Purpose

    Expresses tagged TPC2 in mammalian cells

    Depositor

    Antony Galione

    Article

    Davis et al Curr Biol. 2012 Dec 18;22(24):2331-7. do

    Insert

    TPC2 (TPCN2 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCB094 FoMV-sgLw

    Plasmid

    #234816

    Purpose

    To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbLw (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    gRNA targeting SbLw
    

    Expression

    Plant

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21-HIS-Tol2

    Plasmid

    #112486

    Purpose

    functional recombinant Tol2 protein from E.coli

    Depositor

    Stephen Ekker

    Article

    Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

    Insert

    Tol2 Transposase (tpase Synthetic)
    

    Tags

    HIS/6x

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. ADORA1-Tango

    Plasmid

    #66209

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ADORA1 (ADORA1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-CD40L-mgSrtA

    Plasmid

    #125795

    Purpose

    for co-expression of CD40L (CD40 ligand) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD40 positive cells.

    Depositor

    P. Chen

    Article

    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

    Insert

    CD40L-mgSrtA
    

    Expression

    Mammalian

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2 mCherry-Rtn4a

    Plasmid

    #86683

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    Rtn4a (RTN4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-TetO-hNGN2-Neo

    Plasmid

    #99378

    Purpose

    Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    hNGN2-P2A-Neo (NEUROG2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET His6 GST TEV LIC cloning vector (2G-T)

    Plasmid

    #29707

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPAmCherry1-C1

    Plasmid

    #31929

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Expression

    Mammalian

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    CMV

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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