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TFORF0120

Plasmid

#142539

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ELMSAN1 (MIDEAS Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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acGFP Peroxisome visual barcode

Plasmid

#158674

Purpose

tagging clonal populations with Peroxisomal acGFP fluorescent protein. Adding Peroxisome localization signal to acGFP WC

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

acGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF3137

Plasmid

#144613

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HEY1 (HEY1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CCR8-Tango

Plasmid

#66245

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CCR8 (CCR8 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pMK446 (BSD-P2A-BromoTag-mClover)

Plasmid

#214376

Purpose

BromoTag-mClover for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-BromoTag-mClover

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK447 (BSD-P2A-BromoTag-mCherry)

Plasmid

#214377

Purpose

BromoTag-mCherry for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-BromoTag-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK466 (mAB-BSD)

Plasmid

#214392

Purpose

mAID-BromoTag for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

mAID-BromoTag-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF2195

Plasmid

#142181

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MLXIPL (MLXIPL Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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SunTag-FWAgRNA-4-22aa-TET1cd

Plasmid

#106435

Purpose

SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam E2F3a

Plasmid

#37970

Depositor

Jacqueline Lees

Article

Lees et al Mol Cell Biol. 1993 Dec;13(12):7813-25.

Insert

E2F3a (E2F3 Human)

Expression

Mammalian

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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pSLIK-PUM1-rABE-Flag

Plasmid

#191385

Purpose

Doxycycline inducible expression of PUM1-rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

PUM1-rABE (PUM1 Human, Synthetic)

Use

Lentiviral

Tags

Flag

Promoter

tet-inducible promoter

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pProEx Rac1(G12C) CL

Plasmid

#224269

Purpose

Protein purification of Rac1 (G12C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Expression

Bacterial

Mutation

G12C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLVXPuro-mGFP-Sec23B

Plasmid

#66598

Purpose

Expresses Sec23B as a fusion with mGFP

Depositor

David Stephens

Article

Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

Insert

Sec23B (SEC23B Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b-KSRP

Plasmid

#23019

Depositor

Douglas Black

Article

Min et al Genes Dev. 1997 Apr 15. 11(8):1023-36.

Insert

KSRP (KHSRP Human)

Tags

His

Expression

Bacterial

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hAtg13

Plasmid

#22875

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

autophagy-related genes 13 (ATG13 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
TFORF1202

Plasmid

#144088

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SETDB1 (SETDB1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2(WT)-8his

Plasmid

#149268

Purpose

Mammalian expression plasmid for his-tagged sACE2

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Short GSG linker and 8his tag

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-BRPF1

Plasmid

#65382

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRPF1 (BRPF1 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
mCherry-Keratin14-N-18

Plasmid

#55066

Purpose

Localization: Cytokeratin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Keratin14 (KRT14 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
GST HP1 alpha

Plasmid

#24074

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 alpha (CBX5 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only