We narrowed to 12,202 results for: 112

1. GFP-BLOS1

   Plasmid

   #164625

   Purpose

   Expresses BLOS1 in mammalian cells with a GFP tag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   BLOS1 (BLOC1S1 Human)
   

   Tags

   Cycle 3 GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH_CMV_dimer_mKate2

   Plasmid

   #167333

   Purpose

   Expresses mKate2 fused to TFAM no-dimerization mutant in mammalian cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Lentiviral

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   Changed K95A, Y99F, E106A, E112A and R116A

   Promoter

   CMV

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CCR10-DuET

   Plasmid

   #213197

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CCR10 (CCR10 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)

   Plasmid

   #139990

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   VQR=D1135V/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hsyn-flex-Voltron

   Plasmid

   #119035

   Purpose

   Cre-dependant expression of Voltron in neurons

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

   Insert

   Voltron
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hsyn

   Available Since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-ZDHHC9-C169S

   Plasmid

   #231673

   Purpose

   Expresses human ZDHHC9-C169S protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC9 (ZDHHC9 Human)
   

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)

   Plasmid

   #139991

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   VRER=D1135V/G1218R/R1335E/T1337R

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Low_SynNotch_RHL183

   Plasmid

   #164827

   Purpose

   Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Inserts

   AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor
   

   Use

   Lentiviral

   Tags

   mCherry

   Promoter

   miniCMV and pGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3HAT1Luc1

   Plasmid

   #236275

   Purpose

   Mammalian expression of human angiotensin II type 1 receptor; N-terminal HA tag, C-terminal Rluc8

   Depositor

   Vsevolod Gurevich

   Article

   Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

   Insert

   angiotensin II type 1 receptor (AGTR1 Human)
   

   Tags

   3xHA and Rluc8

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Medium_SynNotch_RHL144

    Plasmid

    #164828

    Purpose

    Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Medium Gal4VP64 Synthetic Notch

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Inserts

    AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    AntiHER2 4D5-Medium Gal4VP64 synthetic Notch receptor
    

    Use

    Lentiviral

    Tags

    mCherry

    Promoter

    miniCMV and pGK

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJM1 FLCN (WT) FLAG GFP

    Plasmid

    #164978

    Purpose

    Lentiviral vector for FLCN expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    FLCN (FLCN Human)
    

    Use

    Lentiviral

    Tags

    1xFLAG-GFP

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-28a RUVBL2 Full Length

    Plasmid

    #159142

    Purpose

    Expresssion and protein purification of full length RUVBL2

    Depositor

    Eric Erquan Zhang

    Article

    Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

    Insert

    RUVBL2 (RUVBL2 Human)
    

    Expression

    Bacterial

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-GRAB_UCN1.0

    Plasmid

    #208669

    Purpose

    Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. MiniCoopR mitfa:BRAF

    Plasmid

    #118846

    Purpose

    Expresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    BRAF V600E (BRAF Human, Zebrafish)
    

    Use

    Zebrafish expression, tol2

    Mutation

    BRAF V600E

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-TRE-DIO-Casp3-TEV

    Plasmid

    #183766

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Expression

    Mammalian

    Promoter

    tetO promoter

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CXCR1-DuET

    Plasmid

    #213218

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR1 (CXCR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CCR3-DuET

    Plasmid

    #213199

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CCR3 (CCR3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-FLAG-HA-CAD S1859A

    Plasmid

    #46239

    Purpose

    FLAG-HA-CAD with S1859 phosphorylation site mutated to alanine

    Depositor

    Brendan Manning

    Article

    Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

    Insert

    carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Serine 1859 changed to Alanine (S1859A)

    Promoter

    cmv

    Available Since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

19. plenti-px330-CRBN-T1-pGK-Pur

    Plasmid

    #107382

    Purpose

    Mammalian expression CRISPR/Cas9

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    sgRNA targeting CRBN (CRBN Human)
    

    Use

    Lentiviral; Crispr

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-GRAB_NPY1.0

    Plasmid

    #208676

    Purpose

    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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