We narrowed to 36,703 results for: ELL
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Plasmid#109230PurposeFor Tol2 transposon-mediated stable expression of LRL and IRES-luciferaseDepositorInsertLRL and Luc2
ExpressionMammalianPromoterCAGGSAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-H_peroxisome_C1
Plasmid#85064PurposeIn vivo visualization of the peroxisome (can be used for colocalization studies)DepositorInsertPeroxisomal targeting sequence
TagsmScarlet-HExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP6f-RS06
Plasmid#67159Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP6f-RS06
Tags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationM374Q within GCaMP6fPromoterT7Available SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pML333_LAMP1-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227898PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertLAMP1 homology arms with mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS
Plasmid#158058PurposeDonor plasmid for chromosomal integrations of any sequence at a locus downstream of the glmS gene in Enterobacteriaceae using kanamycin resistance as a selectable markerDepositorInsertsglmS homologous region 1
glmS homologous region 2 + MCS3
UseSynthetic BiologyAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-EGFP
Plasmid#133400PurposePiggybac vector for constitutive expression of EGFPDepositorInsertEGFP
ExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Smpd4
Plasmid#120237PurposeExpresses V5-tagged Smpd4 in lentiviral vectorDepositorAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
V1-MESA-45F-M-dCas9
Plasmid#84504PurposeMESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64DepositorInsertV2-MESA-35F-M-dCas9
UseLentiviralTagsFlagExpressionMammalianPromoterCMVAvailable SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQCXIX_sh_HOIP/RNF31_#6
Plasmid#75252Purposeretroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction controlDepositorAvailable SinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-MLH1dn-IRES-puroR
Plasmid#207354PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).DepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
Plasmid#176624PurposeTF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3DepositorInsertTRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
UseSynthetic BiologyAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PE SpRY
Plasmid#179082PurposeNOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variantDepositorInsertSpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationA61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…PromoterCAGAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr-6080NES(Epac-sensor)
Plasmid#162487PurposehyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertEpac-sensor (RAPGEF3 Human, Synthetic)
TagsYPet and Turquoise2GLExpressionMammalianPromoterCAGAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT 5N5A (Puro)
Plasmid#154293PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationN322A, N356A, N390A, N424A, N458A mutations; HCF-…PromoterPGKAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v3-8his
Plasmid#149271PurposeMammalian expression plasmid for his-tagged sACE2 high affinity variant 3DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v6-sfGFP
Plasmid#145177PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 6DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-hTRF1
Plasmid#103802PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-hPMLIII
Plasmid#103824PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSH-Csy4-T2A-SaRFN
Plasmid#85755PurposeExpresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).DepositorInsertCsy4-T2A-FokI-SadCas9
UseCRISPRTags3xHA tag, Csy4, and FokI fusion via 36 amino acid…ExpressionMammalianMutationD10A, N580APromoterCAGAvailable SinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1V5-His Snk/Plk2 T239A
Plasmid#22219DepositorInsertSnk/Plk2 (PLK2 Human)
ExpressionMammalianMutationsingle amino acid substitution mutant, threonine …Available SinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only