We narrowed to 11,189 results for: cat.1
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Plasmid#220246PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 2* (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPRKKKR494-499NAAIRSPromoterEF-1αAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
RANLS-IETD-BNES
Plasmid#60972PurposeExpresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).DepositorInsertddRFP A and ddRFP B
TagsNES sequence LALKLAGLDIGS and triplicated NLS seq…ExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT D554N
Plasmid#154283PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2MutationD554N mutation (Ser/Thr Glycosylation Impaired)Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
RPS6KB1 gRNA (BRDN0001145927)
Plasmid#75613Purpose3rd generation lentiviral gRNA plasmid targeting human RPS6KB1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159677PurposePlasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.DepositorInsert[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
ExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
macroH2A1.1-F192V-GFP (pc5114)
Plasmid#223437PurposeMutation phenylalanine 192 to valine (F192V) in macroH2A1.1-GFPDepositorAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-MAVS(trunc Cterm)
Plasmid#221259PurposeExpression of MAVS truncation (393-431) in mammalian cells by retroviral transductionDepositorAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2-mt2
Plasmid#220240PurposeExpression of an EZH2 mutant in insect cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
Tags6x His, MBPExpressionInsectMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterPolyhedrinAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-VIM270-pSM
Plasmid#215871PurposeThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.DepositorInsert3 tandem repeats of target site (complementary to seed seq of passenger strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)
ExpressionMammalianAvailable SinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 W391A pcDNA3
Plasmid#206085Purposeexpression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit bindingDepositorAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK FKBP12(F36V)-OGT (Neo)
Plasmid#154294PurposeLentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoterDepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x HA and FKBP12(F36V)ExpressionMammalianPromoterPGKAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human Wild-type ASAP1
Plasmid#235212PurposeExpresses Wild-Type ASAP1 in mammalian cellsDepositorAvailable SinceJune 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2-mt2
Plasmid#220245PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterEF-1αAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Gal4-HA-HIF1α(530-652)
Plasmid#24887DepositorInserthypoxia inducible factor1alpha (HIF1A Human)
TagsGal4dbd and HA-tagExpressionMammalianMutationpartial HIF1alpha cDNA (encoding aa 530-652)fused…Available SinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_huMcl-1.1
Plasmid#85530PurposeInducible expression of guide RNA (huMcl-1.1) with fluorescent GFP reporterDepositorAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAPK3 gRNA (BRDN0001147876)
Plasmid#75967Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK3DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiMutB3GALT5-blast
Plasmid#208381Purposelentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequenceDepositorInsertbeta-1,3-galactosyltransferase 5 (B3GALT5 Human)
UseLentiviralTagsmyc, FLAGExpressionMammalianMutationnucleotide change in PAM targeting sequence nt 51…PromoterEF-1 alpha core promoterAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-PHD2-181-426
Plasmid#223551PurposeHis-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purificationDepositorInsertPHD2 (EGLN1 Human)
Tags6x HisExpressionBacterialMutationDeleted amino acids 1-180.PromotertrcAvailable SinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
BRAF gRNA (BRDN0001146266)
Plasmid#76480Purpose3rd generation lentiviral gRNA plasmid targeting human BRAFDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL2-hOct4 DE-SV40-Luc
Plasmid#26279PurposeAddgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.DepositorInsertOct4 Distal Enhancer Element (DE) (POU5F1 Human)
UseLuciferaseExpressionMammalianMutationSee Depositor Comments belowAvailable SinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only