165,706 results

1. pCZGY3163

   Plasmid

   #101248

   Purpose

   GFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.

   Depositor

   Yishi Jin

   Article

   Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

   Insert

   gfp11::rps-18 (rps-18 Nematode, Synthetic)
   

   Use

   Gateway

   Tags

   GFP11

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. 7TFP CDH1 reporter

   Plasmid

   #91704

   Purpose

   lentiviral luciferase reporter containing CDH1 promoter

   Depositor

   Bob Weinberg

   Article

   Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

   Insert

   CDH1 promoter (CDH1 Human)
   

   Use

   Lentiviral and Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   CDH1

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH_Str-KDEL_ManII-SBP-EGFP

   Plasmid

   #65258

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Use

   Lentiviral

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. SaCas9 GFP V3

   Plasmid

   #226962

   Purpose

   CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. scFv-GCN4-DNMT3a(R887E)-DNMT3l

   Plasmid

   #154141

   Purpose

   Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
   

   Tags

   HA and sfGFP

   Expression

   Mammalian

   Mutation

   R887E

   Promoter

   SFFV

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOSIP-KH-RBS2-dCas9

   Plasmid

   #182740

   Purpose

   Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

   Depositor

   David Bikard

   Article

   Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

   Insert

   dCas9
   

   Use

   CRISPR; Integrative vector

   Promoter

   Ptet

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. amilCP chromoprotein

   Plasmid

   #117847

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amilCP
   

   Use

   Synthetic Biology; Escherichia coli

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PKC zeta DN

   Plasmid

   #21249

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC zeta (Prkcz Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K281M

   Available Since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

9. 7TGP

   Plasmid

   #24305

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-eGFP // SV40-PuroR
   

   Use

   Lentiviral

   Mutation

   EGFP is GFP with S65T and F64L mutations

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. YPet-N1

    Plasmid

    #54637

    Purpose

    Localization: N1 Cloning Vector, Excitation: 513, Emission: 527

    Depositor

    Patrick Daugherty, Michael Davidson

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

    Type

    Empty backbone

    Tags

    YPet

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. HDAC4 Flag

    Plasmid

    #13821

    Purpose

    Mammalian expression of human histone deacetylase 4 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC4 (HDAC4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAS

    Plasmid

    #60847

    Purpose

    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

    Depositor

    Jamie Cate

    Article

    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

    Inserts

    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS/His8 and TTT 3' extension prior to sgRNA

    Expression

    Bacterial and Yeast

    Mutation

    L4 is UUCG tetraloop and guide targets LYP1 (CATA…

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET30a-Tn5

    Plasmid

    #127917

    Purpose

    Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.

    Depositor

    Silin Zhong

    Article

    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

    Insert

    Tn5
    

    Tags

    Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    T7

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+i)

    Plasmid

    #192123

    Purpose

    Lentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(d+i)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_41BB_NS3(s139a)_CD3z-mCherry
    

    Use

    Lentiviral

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFlagCMV2-UbcH8

    Plasmid

    #12442

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

    Insert

    UbcH8 (UBE2L6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

16. mTagBFP2-MannII-N-10

    Plasmid

    #55309

    Purpose

    Localization: Mannosidase/Golgi, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    MannII
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    aa 1-112 of MannII

    Promoter

    CMV

    Available Since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pDONR223_RAF1_WT

    Plasmid

    #82317

    Purpose

    Gateway Donor vector containing RAF1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. iOn-CAG∞MCS

    Plasmid

    #154013

    Purpose

    Expression vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with an MCS to clone-in genes of interest and express it from a CAG promoter

    Depositor

    Jean Livet

    Article

    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

    Insert

    MCS
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. FUW

    Plasmid

    #14882

    Depositor

    David Baltimore

    Article

    Lois et al Science. 2002 Feb 1. 295(5556):868-72.

    Insert

    flap-Ub promoter-WRE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK-flag-USP19

    Plasmid

    #78597

    Purpose

    Express flag-USP19 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 (USP19 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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