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pEGFP-WRAP53beta-N-terminus

Plasmid

#64677

Purpose

expresses only N terminal o WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

Only N terminus amino acids 1-160

Promoter

CMV

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-C-terminus

Plasmid

#64679

Purpose

expresses only C terminus of WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

only C terminus amino acids 500-548

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pET41a-MED31

Plasmid

#15437

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED31 (Med31 Mouse)

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-Med11 pBacPAK8

Plasmid

#15373

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med11 (Med11 Mouse)

Use

Baculovirus construction

Tags

FLAG

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
myc-Med31 pBacPAK8

Plasmid

#15375

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med31 (Med31 Mouse)

Use

Baculovirus construction

Tags

myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
myc-Med19 pBacPAK8

Plasmid

#15376

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med19 (Med19 Mouse)

Use

Baculovirus construction

Tags

myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
aRJ_00134_PGK-mNeonGreen-syn_pA-EF1alpha-TagBFP-syn_pA

Plasmid

#252538

Purpose

Constitutive promoters with downstream tandem syntax for PiggyBac integration

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

TagBFP

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), hPGK (hu…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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aRJ_00135_EF1alpha-TagBFP-syn_pA-PGK-mNeonGreen-syn_pA

Plasmid

#252539

Purpose

Constitutive promoters with upstream tandem syntax for PiggyBac integration

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

TagBFP

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), hPGK (hu…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01701_CMV-rtTA-betaglobin_pA-CMV-PuroR-bGH

Plasmid

#252540

Purpose

Secondary PiggyBac vector for puromycin selection and expression of transcriptional activator

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

rtTA

Expression

Mammalian

Promoter

CMV (human cytomegalovirus immediate early enhanc…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01416_TRE-TagBFP-bGH-EF1alpha-mRuby2-SV40

Plasmid

#252541

Purpose

Inducible gene and constitutive reporter with downstream tandem syntax, used to generate monoclonal PiggyBac-integrated lines

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

mRuby2

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), Tight TR…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01417_TRE-TagBFP-bGH-(EF1alpha-mRuby2-SV40)

Plasmid

#252542

Purpose

Inducible gene and constitutive reporter with convergent syntax, used to generate monoclonal PiggyBac-integrated lines

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

mRuby2

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), Tight TR…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01418_(TRE-TagBFP-bGH)-EF1alpha-mRuby2-SV40

Plasmid

#252543

Purpose

Inducible gene and constitutive reporter with divergent syntax, used to generate monoclonal PiggyBac-integrated lines

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

mRuby2

Expression

Mammalian

Promoter

hEF1a (human elongation factor 1-alpha), Tight TR…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKG02752_pLentiX1-EFS-rtTA-P2A-mGL-WPRE

Plasmid

#252548

Purpose

Lentiviral expression of the rtTA transcriptional activator with a fluorescent reporter

Depositor

Kate Galloway

Article

Johnstone et al bioRxiv [Preprint]. 2025 Jan 19:2025.01.

Insert

rtTA-P2A-mGreenLantern

Use

Lentiviral

Expression

Mammalian

Promoter

EFS (human elongation factor 1-alpha - short/intr…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP-ATG13-1-230(HF|DD)

Plasmid

#250344

Purpose

Expressing human ATG13 (1-230) with H214D/F215D mutations

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ATG13 (ATG13 Human)

Tags

GFP

Expression

Mammalian

Mutation

only amino acids 1-230 with H214D and F215D muta…

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-TSF-ULK1

Plasmid

#249533

Purpose

Expressing human full length ULK1

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

Flag-tag and strep-tag

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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rDNA-adj gRNA #3

Plasmid

#247549

Purpose

negative control for Crispri KD

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

negative control sgRNA targeting region adjacent to 5' end of ribosomal DNA repeats of distal junction

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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rDNA-adj gRNA #1

Plasmid

#247548

Purpose

negative control for Crispri KD

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

negative control sgRNA targeting region adjacent to 5' end of ribosomal DNA repeats of distal junction

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-myo1e-tail

Plasmid

#248919

Purpose

Expresses EGFP-tagged human myosin 1e tail (aa 710-1109) in mammalian cells

Depositor

Mira Krendel, Mark Mooseker

Article

Krendel et al FEBS Lett. 2007 Feb 20;581(4):644-50. do

Insert

Myo1e tail (MYO1E Human)

Tags

EGFP

Expression

Mammalian

Mutation

contains only amino acids 710 to the end

Promoter

CMV

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-ALFA-bRho-EGFP

Plasmid

#248792

Purpose

Expresses ALFA-bRho-EGFP in mammalian cells. Modified from Addgene plasmid #45399

Depositor

Michael Robichaux

Article

Hekmatara et al bioRxiv [Preprint]. 2025 Sep 25:2025.09.

Insert

RHO (RHO Bovine)

Tags

ALFA and EGFP

Expression

Mammalian

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-T2A-mNeonGreen-2-bc6

Plasmid

#250524

Purpose

Expresses Cas9 and mNeonGreen. Barcode is added to the vector.

Depositor

James Eshleman

Article

Teh et al bioRxiv [Preprint]. 2023 Apr 5:2023.04.0

Insert

M13F-AATTGGCC

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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