165,706 results

1. pRT801

   Plasmid

   #68112

   Purpose

   Integration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.

   Depositor

   Maggie Smith

   Article

   Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

   Inserts

   aac3(IV)
   oriT
   attP
   int
   

   Use

   Streptomyces integrating vector

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-Rab5a-7

   Plasmid

   #55126

   Purpose

   Localization: Ras GTPase, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Rab5a
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. CbS-Flag-TRRAP

   Plasmid

   #32103

   Depositor

   Yardena Samuels, Michael Cole

   Insert

   Transformation/transcription domain-associated protein (TRRAP Human)
   

   Tags

   Flag 1x

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRH029

   Plasmid

   #171060

   Purpose

   Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   Gag-spyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x SV40 NLS and 3x FLAG tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRN3P_T3_ABE8e_IVT

   Plasmid

   #201676

   Purpose

   Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72

   Insert

   ecTadA(8e)-nSpCas9
   

   Use

   CRISPR; Vector for in-vitro transcription

   Promoter

   T3 promoter

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-KLF5

   Plasmid

   #40918

   Depositor

   Jin-Tang Dong

   Article

   Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

   Insert

   Kruppel-like factor 5 (KLF5 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV14_Fibrillarin

   Plasmid

   #73061

   Purpose

   expression clone for human Fibrillarin with C-terminal 3xFLAG-tag

   Depositor

   Stefan Stamm

   Insert

   Fibrillarin (FBL Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX304 YAP1

   Plasmid

   #193689

   Purpose

   Constitutive lentiviral expression of YAP1

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-U6/sgClta-sfGFP-Clta

   Plasmid

   #200261

   Purpose

   U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

   Depositor

   Justin Eyquem

   Article

   Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

   Insert

   sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta
   

   Use

   AAV

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7-VP1SA11

    Plasmid

    #89162

    Purpose

    Express Rotavirus SA11 VP1 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP1
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti_EF1a_Flag_TP53_WT

    Plasmid

    #183112

    Purpose

    Expresses flag-tagged p53 in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PMXS-NDI1

    Plasmid

    #72876

    Purpose

    expresses NDI1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

    Insert

    NDI1 (NDI1 Budding Yeast)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNatB (pACYCduet-naa20-naa25)

    Plasmid

    #53613

    Purpose

    Allows expression of the fission yeast NatB complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

    Inserts

    naa20 (naa20 Fission Yeast)
    naa25 (naa25 Fission Yeast)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDY0209 ACTB Nicking guide +48

    Plasmid

    #179109

    Purpose

    ACTB N-term Nicking guide 1 +48 guide

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term Nicking guide 1 +48 guide
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3 WT B-Myb

    Plasmid

    #25965

    Depositor

    Rob Lewis

    Article

    Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

    Insert

    B-Myb (MYBL2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-FBXL17

    Plasmid

    #236188

    Purpose

    Expression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.

    Depositor

    Michele Pagano

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    FLAG-FBXL17 (FBXL17 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. PKC eta DN

    Plasmid

    #21246

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC eta (Prkch Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K384R

    Available Since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1_ADAR2DD_E488Q_Ago2

    Plasmid

    #205599

    Purpose

    expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) fused with human Ago2 in mammalian cells

    Depositor

    Marc Friedlander

    Article

    Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

    Insert

    hAgo2 (AGO2 Human)
    

    Tags

    ADAR2DD_E488Q (hyperactive mutant)

    Expression

    Mammalian

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-tdPP7-3xmCherry

    Plasmid

    #74926

    Purpose

    Expresses tdPP7-3xmCherry

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    PP7-3xmCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-N-Blast-ultraID

    Plasmid

    #207682

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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