169,365 results

Showing: 10521 - 10540 of 169365 results

Results per page:

Sort results by:

pCMV-Frankenbody-FLAG-Halo

Plasmid

#175296

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with HaloTag

Tags

HaloTag

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGEX-GST-His6-2_HBD

Plasmid

#171870

Purpose

Expresses tandem repeats of HBD domain of RNase H1 for better DNA-RNA Hybrid recognition specificity in bacterial cells

Depositor

Kaiwei Liang

Article

Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

Insert

Tandem repeats Hybrid binding domain of Rnase H1

Tags

GST- and His10-tag

Expression

Bacterial

Mutation

WT

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-dCas9-VP64

Plasmid

#47107

Purpose

Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Tags

Flag, HA, SV40 NLS, and VP64

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDOXOFF 15xGly

Plasmid

#211345

Purpose

DOX-regulated lentiviral expression of 15xGly-EGFP-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

15xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGW-TurboID-GOI-GFP

Plasmid

#209383

Purpose

Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Expression

Plant

Mutation

TurboID gene sequence C249A

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Go1-CASE

Plasmid

#168123

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

Depositor

Gunnar Schulte

Article

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Insert

GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)

Use

Luciferase

Tags

cpVenus on GNG9

Expression

Mammalian

Mutation

NLuc is inserted at L91/G92 within GNAO1

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-R-GECO1

Plasmid

#32444

Purpose

Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

R-GECO1.0

Expression

Mammalian

Mutation

Substitutions relative to the mApple-derived anal…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-HA-BAX

Plasmid

#16587

Depositor

Bert Vogelstein

Article

Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Insert

BAX alpha (BAX Human)

Tags

2x HA

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pfwB

Plasmid

#37329

Depositor

John Schiller

Article

Buck et al J Virol. 2005 Mar;79(5):2839-46.

Insert

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

July 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB-TRE-CRISPRoff-EF1A-TetOn

Plasmid

#203355

Purpose

Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

Depositor

Jonathan Brunger

Article

Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

Insert

CRISPRoff-TetOn

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-N1

Plasmid

#54567

Purpose

Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Tags

mApple

Expression

Mammalian

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_MAX

Plasmid

#234044

Purpose

Bacterial expression of N-terminally 6His-tagged MAX; includes TEV cleavage site

Depositor

Tanja Mittag

Article

Yang et al Nat Struct Mol Biol. 2024 Oct;31(10):156

Insert

MAX (MAX Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-Flag-humanRBM47

Plasmid

#134607

Purpose

Plasmid bearing RBM47 coding sequence. Flag tagged is useful for IP and WB assays.

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

Flag-humanRBM47 (RBM47 Human)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mTurquoise2-LaminB

Plasmid

#98830

Purpose

In vivo visualization of the nuclear envelope (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

LaminB

Tags

mTurquoise2

Expression

Mammalian

Mutation

*(see below)

Promoter

CMV

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p221a-GUS

Plasmid

#71263

Purpose

Entry clone containing the GUS enzyme. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

Beta-glucuronidase

Use

Gateway

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-PRMT6

Plasmid

#164697

Purpose

Expression of PRMT6 with an N-terminal FLAG tag

Depositor

Cheryl Arrowsmith

Article

Eram et al ACS Chem Biol. 2016 Mar 18;11(3):772-781

Insert

PRMT6 (PRMT6 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-LoxP-mKate-LoxP-EGFP

Plasmid

#167940

Purpose

CMV promoter-LoxP-mKate-LoxP-EGFP

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

LoxP-mKate-LoxP-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pROP-PP-GFP

Plasmid

#122135

Purpose

GFP Reporter plasmid for B-Rex contain perfect operator sequence

Depositor

Srivatsan Raman

Article

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

GFP

Expression

Bacterial

Promoter

J23101

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUB_smFLAG_KDM5B_MS2

Plasmid

#81084

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smFLAG_KDM5B_24xMS2

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge