165,725 results

1. pcDNA3.1-INSR-V5/HIS

   Plasmid

   #201980

   Purpose

   expression of human INSR receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   INSR receptor tyrosine kinase (INSR Human)
   

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

   Plasmid

   #84257

   Purpose

   Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sp dCas9-ABI
   GID1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

   Expression

   Mammalian

   Promoter

   CAG and PGK

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. GPR158-Tango

   Plasmid

   #66332

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR158 (GPR158 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCBC-MT3T4

   Plasmid

   #50595

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T3, gRNA scaffold, U6-26t plus, OsU3p, T4
   

   Use

   CRISPR; Pcr template

   Expression

   Plant

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHJL2

   Plasmid

   #199377

   Purpose

   Encodes dual cistronic cytosolic GCaMP6f and mScarlet-I optimized for C. elegans expression

   Depositor

   Hang Lu

   Insert

   GCaMP6f::SL2::mScarlet-I
   

   Expression

   Bacterial

   Mutation

   no mutations

   Available Since

   April 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pLJM1-FLAG-metap2

   Plasmid

   #100510

   Purpose

   Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   METAP2 (METAP2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-Cdk9

   Plasmid

   #28102

   Depositor

   Andrew Rice

   Article

   Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

   Insert

   Cdk9 (CDK9 Human)
   

   Tags

   3x HA

   Expression

   Bacterial and Mammalian

   Available Since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-dLight1.2

   Plasmid

   #111068

   Purpose

   To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Has Service

   AAV1 and AAV5

   Insert

   dLight1.2
   

   Use

   AAV

   Tags

   Flag tag

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MELOPS

   Plasmid

   #80151

   Purpose

   Genetically encoded melanosome-localized pH sensor.

   Depositor

   Santiago Di Pietro

   Article

   Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

   Insert

   OCA2 (OCA2 Human)
   

   Tags

   mNectarine in first luminal loop

   Expression

   Mammalian

   Mutation

   nonpathologic polymorphism D257A, pore mutation V…

   Promoter

   CMV

   Available Since

   Sept. 20, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pX037

    Plasmid

    #167156

    Purpose

    MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    caffeic acid cycle genes
    

    Expression

    Plant

    Promoter

    pNOS, p35s

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSH83

    Plasmid

    #212989

    Purpose

    expresses miniSOG in C. elegans

    Depositor

    David M. Miller

    Article

    Cuentas-Condori et al Elife. 2019 Oct 4;8. pii: 47918. doi: 10

    Insert

    flp-13 promoter (flp-13 Nematode)
    

    Expression

    Worm

    Promoter

    flp-13

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast

    Plasmid

    #205550

    Purpose

    Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-MCP
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG mito-RCaMP1h

    Plasmid

    #105013

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    RCaMP1h
    

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas3-Amp

    Plasmid

    #207998

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    

    Expression

    Mammalian

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNitET-SacB-kan

    Plasmid

    #107692

    Purpose

    Expression of the phage Che9c RecET system for Recombineering in mycobacteria

    Depositor

    Kenan Murphy

    Article

    Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

    Inserts

    Che9 phage RecET
    PNit2-NitR
    SacRB
    

    Expression

    Bacterial

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223-ADRBK2

    Plasmid

    #23793

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ADRBK2 (AA1-387) (GRK3 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. IgK-IFN-luc

    Plasmid

    #14886

    Purpose

    Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression

    Depositor

    David Baltimore

    Article

    Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.

    Insert

    IgK-IFN (IFNB1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    May 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMEH380 VSV-G HA

    Plasmid

    #168222

    Purpose

    Mammalian expression of vesicular stomatitis virus glycoprotein fused to a C-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    VSV-G
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-MET-V5/HIS

    Plasmid

    #201982

    Purpose

    expression of human MET receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    MET receptor tyrosine kinase (MET Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBad33_ISDge10_reRNA

    Plasmid

    #205340

    Purpose

    Bacterial expression of ISDge10 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISDge10 TnpB
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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