We narrowed to 13,992 results for: CAR

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rag2:AVIL_CE_pISceI

Plasmid

#223032

Purpose

Express AVIL gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

AVIL (AVIL Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:HMGA2_CE_pISceI

Plasmid

#223034

Purpose

Express HMGA2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

HMGA2 (HMGA2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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KHC065 pBABE pU6 BRD2 K1-2C

Plasmid

#231712

Purpose

KHC065 (pBABE pU6 BRD2 K1-2C), gRNA and cas9D10A mammalian expression vector for CRISPR mediated cutting of BRD2 at the N-terminus, use with KHC064

Depositor

Alessio Ciulli

Article

Bond et al J Med Chem. 2021 Oct 28;64(20):15477-155

Insert

gRNA for Brd2 and Cas9D10A (BRD2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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KHC064 pX335 BRD2 K1-1a

Plasmid

#231711

Purpose

KHC064 (pX335 BRD2 K1-1a), gRNA and cas9D10A mammalian expression vector for CRISPR mediated cutting of BRD2 at the N-terminus, use with KHC065

Depositor

Alessio Ciulli

Article

Bond et al J Med Chem. 2021 Oct 28;64(20):15477-155

Insert

gRNA for Brd2 and Cas9D10A (BRD2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pML390_YWHAQ-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227925

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

YWHAQ homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML389_YWHAB-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227926

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

YWHAB homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML600_COG8-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227911

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

COG8 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML357_CAV1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227914

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CAV1 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML361_DCP1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227918

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

DCP1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML366_HSPA1B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227920

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

HSPA1B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML365_HSP90AA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227921

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

HSP90AA homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML364_PSMB7-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227922

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

PSMB7 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML374_CLTA-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227893

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CLTA homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML384_NCLN-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227897

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

NCLN homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML318_SEC23A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227903

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

SEC23A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbleo-TGFa-EREG-ScNeo

Plasmid

#209915

Purpose

To express the chimeric protein of TGFα and EREG, which a recombinant TGFα protein fused extracellularly to the mScarlet and a recombinant Epiregulin protein fused intracellularly to the mNeonGreen

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-EREG-ScNeo (EREG, TGFA Human)

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

Plasmid

#163701

Purpose

Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Inserts

SOX2-3xHA-P2A-tagBFP (SOX2 Human)
rtTA
T2-sgRNA

Use

AAV and CRISPR

Tags

3x-HA and P2A-tagBFP

Expression

Mammalian

Promoter

CAG and TRE3G

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-EZH1-Y727F

Plasmid

#203582

Purpose

Expresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.

Depositor

Abhishek Chakraborty, William Kaelin

Article

Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5

Insert

EZH1 (EZH1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

changed Tyrosine 727 to Phenylalanine for partial…

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-EZH1-dPSET

Plasmid

#203586

Purpose

Expresses V5-tagged mutant version of EZH1 with deletion of aa 744-747 and partially resistant to JQ-EZ-05 in mammalian cells.

Depositor

Abhishek Chakraborty, William Kaelin

Article

Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5

Insert

EZH1 (EZH1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

deletion of aa 744-747 and partial resistance to …

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-EZH1-D745N

Plasmid

#203585

Purpose

Expresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.

Depositor

Abhishek Chakraborty, William Kaelin

Article

Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5

Insert

EZH1 (EZH1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

changed Aspartic Acid 745 to Asparagine for parti…

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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