We narrowed to 11,529 results for: Kars;
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Plasmid#18693DepositorInsertH-Ras (HRAS Human)
Tagsphotoactivatable GFPExpressionMammalianMutationA206K mutation in paGFPAvailable SinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28a-B4E
Plasmid#162067PurposeFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4EDepositorInsertampR-eIF4E fusion protein
Tags6xHis tagExpressionBacterialMutationeIF4E incorporating the K119A mutation, which is …PromoterT7Available SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCB6-Cor1-HA
Plasmid#102815PurposeExpression of Coronin1 in eukaryotic cellsDepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-SI-112
Plasmid#131127PurposeC-to-T base editing reporterDepositorInsertMutant EGFP
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterCMV promoterAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-rnc-sfGFP
Plasmid#51560PurposeGene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).DepositorInsertrnc (rnc E. coli)
TagsGLESTCRHASLAVLADERRFSA and superfolder GFPExpressionBacterialMutationContains additonal aa at the end of the sfGFP in …Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6A CARD8 CARD-FLAG WT
Plasmid#164020PurposeMammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tagDepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGTag-P2A-QFGal4-SV40pA
Plasmid#155127PurposeDonor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld methodDepositorInsertP2A-QFGal4
UseCRISPR and Synthetic BiologyAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8
Plasmid#169962PurposepDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorAvailable SinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT/FLAG/HA-DEST EIF2C4
Plasmid#19890DepositorInsertEukaryotic translation initiation factor 2C, 4 (AGO4 Human)
TagsFLAG/HAExpressionMammalianAvailable SinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET24a HAB1 (172-511)
Plasmid#177844PurposeBacterial Expression of HAB1DepositorInsertProtein phosphatase 2C 16
Tags6xHis-GSTExpressionBacterialAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMSCVpuro
Plasmid#134983PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-LAP1_2-mCherry
Plasmid#159526PurposeExpresses mCherry from LAP1_2 promoterDepositorInsertmCherry
UseAAVExpressionMammalianPromoterLAP1_2Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-TetR-KRf-TetR
Plasmid#216270PurposeConstruct 3: TetR - Lipid binding domain KRφ - TetRDepositorInsertTetR - Lipid binding motif (KRf) - TetR
TagsEGFPExpressionMammalianAvailable SinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGATOR
Plasmid#64102PurposeTet inducible, Gateway adapted ROSA26 targeting VectorDepositorInsertrTTA (Gt(ROSA)26Sor Synthetic)
UseMouse Targeting; Tet inducibleExpressionMammalianPromoterROSA26 endogenousAvailable SinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDNA3-Cerulean-FHA1-NES
Plasmid#138202PurposeEncodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.DepositorInsertCerulean-FHA1-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-ING1b
Plasmid#79052Purposeexpression of p33ING1b in mammalian cellsDepositorAvailable SinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKCsub-YPet-NES
Plasmid#138207PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertPKCsub-YPet-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TO-HA-SH3BP1 (RNAi-res)
Plasmid#136645PurposeExpression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUCDepositorInsertSH3BP1 (RNAi resistant) (SH3BP1 Human)
TagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationGATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTCAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 R443A
Plasmid#162652PurposeExpresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
TagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acid R443 mutated to alaninePromoterCMVAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROF394
Plasmid#155333PurposeExpression of PULSE-controlled LFY-VP16-2A-RLucDepositorInsert(etr)8-(C120)5-PhCMVmin-LFY-VP16-NLS-2A-RLuc-TSV40
UseSynthetic Biology; Eukaryotic expressionAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only