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MZB211
Plasmid#228494Purpose10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)DepositorInsertpol
UseTags10xHis-SUMO(Smt3)ExpressionBacterialMutationA408SPromoterT7 PromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGLO-Magenta-AZA
Plasmid#220784PurposeExpresses a magenta chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)DepositorInsertmagenta chromoprotein
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSYN-TdTomato
Plasmid#193019PurposeAAV vector plasmid expressing TdTomato under the human synapsin (hSYN) promoterDepositorInsertTdTomato
UseAAVTagsExpressionMammalianMutationPromoterhSYNAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
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mu-GM-CSF-pCIneo
Plasmid#163518PurposeExpresses murine GM-CSF in mammalian cellsDepositorInsertmurine GM-CSF
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGM1192
Plasmid#115683PurposeE. coli-Streptomyces shuttle vector for gene expressionDepositorInsertmCherry, aac(3)IV, tsr
UseTagsStrep-tagII on mCherry, codon optimizedExpressionBacterialMutationmCherry codon optimized for StreptomycesPromoterSF14*Available sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-LY-MT-P2A
Plasmid#214897PurposeExpresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sitesDepositorInsertSPLICSFAST-LY-MT-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-B19L
Plasmid#59922PurposeExpression vector for Rabies virus SAD B19 RNA dependent RNA polymerase geneDepositorInsertRabies virus RNA dependent RNA polymerase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn1-eNpHR3.0-iRFP713
Plasmid#197594PurposeLentiviral transfer vector for the neuronal expression of eNpHR3.0-iRFP713 (halorhodopsin for the inhibition of neuronal activity)DepositorInserteNpHR3.0-iRFP713
UseLentiviralTagsExpressionMutationPromoterhSynIAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pSaGuide
Plasmid#64710PurposeCloning and expression of Staphylococcus aureus guide RNADepositorInsertStaphylococcus aureus guide RNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-PO-MT-P2A
Plasmid#214899PurposeExpresses equimolar Peroxisome- and Mitochondria- targeted split-FAST fragments for Short-range PO-MT Contact sitesDepositorInsertSPLICSFAST-PO-MT-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)/MBP-His
Plasmid#38006DepositorInsertMBP (Maltose binding protein)
UseTags6xHISExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 ShhN
Plasmid#37680DepositorInsertshh (Shh Mouse)
UseTagsExpressionMammalianMutationsoluble form (see comments)PromoterCMVAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJA5 H-2K(b)
Plasmid#180441PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2K(b)
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP rbFOX1
Plasmid#63085PurposeMammalian expression of EGFP tagged FOX1DepositorInsertRBFOX1 (RBFOX1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn1-mClover3/CAAX
Plasmid#197591PurposeLentiviral transfer vector for the neuronal expression of membrane-bound mClover3 (good visualization of neuronal processes)DepositorInsertmClover3-CAAX
UseLentiviralTagsCAAXExpressionMutationPromoterhSynIAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-NU-MT-P2A
Plasmid#214898PurposeExpresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sitesDepositorInsertSPLICSFAST-NU-MT-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-Katushka2
Plasmid#99266PurposeContains a fusion of human histone H2B to fluorescent protein Katushka2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertKatushka2-3XHA
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
RHPMP192 (pLifeAct sfGFP)
Plasmid#88843PurposeLocalization by Fluorescence microscopy of lifeAct fused to super folder GFPDepositorInsertLifeAct
UseTagssfGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX
Plasmid#52421PurposeExpresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherryDepositorInsertFusion NLS-EGFP-P2A-Cherry-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pExp-MBP
Plasmid#112568PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-MBP and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEC1.2/EC1.1_spCas9_GFP
Plasmid#161933PurposeCRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSRKBB-empty
Plasmid#59449PurposeContains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromotermodified lacAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYZ205
Plasmid#104586PurposeExpress SNAP-6His-CBDDepositorInsertSNAP-6His-CBD
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti_TetOn_Hu_HEC1(WT)
Plasmid#136353PurposeLentiviral inducible expression (TetOn) of human HEC1DepositorInsertHEC1 (NDC80 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcMscL
Plasmid#92418PurposeE. coli Mechanosensitive Channel of Large ConductanceDepositorInsertMscL (mscL E. coli)
UseTags6x Histidine Tag and Thrombin Cleavage Site betwe…ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRVdG-4RFP-5premGRASP
Plasmid#52499PurposeExpresses TagRFP-T and presynaptic mGRASP componentDepositorInsertsTagRFP-T
presynaptic mGRASP
UseRabies virusTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRVdG-4BFP2-5postmGRASP
Plasmid#52498PurposeExpresses mTagBFP2 and postsynaptic mGRASP componentDepositorInsertsmTagBFP2
postsynaptic mGRASP
UseRabies virusTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-TagRFP-T
Plasmid#99271PurposeContains a fusion of human histone H2B to fluorescent protein TagRFP-T in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertTagRFP-T
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
HT142_pAAV_EF1a_fDiO_Voltron2
Plasmid#208607PurposeExpressing Voltron2 in a Flp-dependent mannerDepositorInsertVoltron2
UseAAVTagsHalo tagExpressionMutationPromoterEF1aAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn1-TagBFP2/CAAX
Plasmid#197590PurposeLentiviral transfer vector for the neuronal expression of membrane-bound TagBFP2 (good visualization of neuronal processes)DepositorInsertmTagBFP2-CAAX
UseLentiviralTagsCAAXExpressionMutationPromoterhSynIAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-gRNA-TnT-Cre
Plasmid#132551PurposeFor loss-of-function experiments in cardiomyocytesDepositorInsertsTnT-Cre
U6-gRNA
UseAAVTagsExpressionMutationPromoterTnT and U6Available sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
proinsulin-msGFP2 (C48S C70S)
Plasmid#218956PurposeExpresses proinsulin fused to msGFP2 (C48S C70S) in bacteriaDepositorInsertproinsulin-msGFP2 C48S C70S
UseTagsproinsulinExpressionBacterialMutationPromoterT5Available sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLO-Pink-SHE
Plasmid#220781PurposeExpresses a raspberry chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)DepositorInsertraspberry chromoprotein (mRFP1)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTCF120 H-2D(b)
Plasmid#180454PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2D(b)
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCitrine-Peroxisomes-2
Plasmid#54672PurposeLocalization: Peroxisomes, Excitation: 516, Emission: 529DepositorTypeEmpty backboneUseTagsPeroximal Targeting Signal 1 (PTS1) and mCitrineExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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mEos4b-NPHP4-N-18
Plasmid#62777PurposeLocalization: Cilium, N1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581DepositorInsertNPHP4
UseTagsmEos4bExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits
