Addgene

We narrowed to 37,659 results for: KAN

Showing: 2601 - 2650 of 37659 results

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pDL25_Cas9-His Δcys E1207C

Plasmid

#206286

Purpose

Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, C574S, E1207C

Promoter

T7

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL23_Cas9-His Δcys E945C

Plasmid

#206284

Purpose

Bacterial expression of Cas9-His Δcys E945C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, C574S, E945C

Promoter

T7

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL24_Cas9-His Δcys E1026C

Plasmid

#206285

Purpose

Bacterial expression of Cas9-His Δcys E1026C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, C574S, E1026C

Promoter

T7

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL27_Cas9-His Δcys E532C/E945C

Plasmid

#206288

Purpose

Bacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, E532C, C574S, E945C

Promoter

T7

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL26_Cas9-His Δcys M1C/E532C

Plasmid

#206287

Purpose

Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

M1C, C80S, E532C, C574S

Promoter

T7

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL22_Cas9-His Δcys E532C

Plasmid

#206283

Purpose

Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, E532C, C574S

Promoter

T7

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP24_T24_pRham-ilvA-relE

Plasmid

#201531

Purpose

Expressing synthetic overlapping gene, ilvA-relE. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PrhaBAD (rhamnose)

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJUMP24_T24_pRham-ilvA-relE_RBS3

Plasmid

#201532

Purpose

Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE_RBS3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PrhaBAD (rhamnose)

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET-GFP-9S [rpoA]

Plasmid

#176221

Purpose

Expression of GFP-9S [rpoA]

Depositor

Ian Small

Article

Bernath-Levin et al Synth Biol (Oxf). 2021 Dec 23;7(1):ysab0

Insert

GFP-9S [rpoA]

Use

Synthetic Biology

Tags

His-GFP

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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1056D

Plasmid

#183132

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1056E

Plasmid

#183133

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[dsx]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1056F

Plasmid

#183134

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[tra]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
BicC.Ca9.R

Plasmid

#168295

Purpose

Expresses Cas9 in Drosophila late germline cells

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

BicC.Cas9-T2A-eGFP-p10

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1046E=dgRNAs[traB,bTub]

Plasmid

#149425

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Insert

U6.3-gRNAs[TraB, bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6.3

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mt-DdCBE G1333-C

Plasmid

#174561

Purpose

mitochondrial-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1333-C

Use

Tags

FLAG

Expression

Plant

Mutation

Promoter

PcUbi

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hNKD2

Plasmid

#174329

Purpose

encodes human NKD2 cDNA with a C-terminal MycHis Tag.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Use

Tags

MycHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mt-DdCBE G1397-C

Plasmid

#174563

Purpose

mitochondrial-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-C

Use

Tags

FLAG

Expression

Plant

Mutation

Promoter

PcUbi

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mt-DdCBE G1397-N

Plasmid

#174562

Purpose

mitochondrial-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-N

Use

Tags

HA

Expression

Plant

Mutation

Promoter

PcUbi

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mt-DdCBE G1333-N

Plasmid

#174560

Purpose

mitochondrial-targeting DdCBE (split DddA, G1333-N) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1333-N

Use

Tags

HA

Expression

Plant

Mutation

Promoter

PcUbi

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
cp-DdCBE G1397-C

Plasmid

#174559

Purpose

Chloroplast-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-C

Use

Tags

FLAG

Expression

Plant

Mutation

Promoter

PcUbi

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
cp-DdCBE G1397-N

Plasmid

#174558

Purpose

Chloroplast-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-N

Use

Tags

HA

Expression

Plant

Mutation

Promoter

PcUbi

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-tetpA(R1R4)-iRPT

Plasmid

#154882

Purpose

Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

Depositor

Hideyuki Okano

Article

Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

Inserts

iRFP713
Puromycin resistance gene
rtTAM2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-DEST-R5R4-pNeo

Plasmid

#141058

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Neomycin resistance gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK8_005

Plasmid

#123885

Purpose

Encodes the F1 Kanamycin resistance cassette as a type 8 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-KanR-ColE1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM535

Plasmid

#84227

Purpose

Disturb the expression of miR535 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR535

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
Osa-pCAMBIA-1301-STTM820

Plasmid

#84228

Purpose

Disturb the expression of miR820 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR820

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM827

Plasmid

#84229

Purpose

Disturb the expression of miR827 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR827

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM1425

Plasmid

#84230

Purpose

Disturb the expression of miR1425 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR1425

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM1430

Plasmid

#84231

Purpose

Disturb the expression of miR1430 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR1430

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM1432

Plasmid

#84233

Purpose

Disturb the expression of miR1432 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR1432

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM1861

Plasmid

#84234

Purpose

Disturb the expression of miR1861 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR1861

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
Osa-pCAMBIA-1301-STTM5144

Plasmid

#84235

Purpose

Disturb the expression of miR5144 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR5144

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM5338

Plasmid

#84236

Purpose

Disturb the expression of miR5338 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR5338

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM5794

Plasmid

#84237

Purpose

Disturb the expression of miR5794 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR5794

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM164

Plasmid

#84196

Purpose

Disturb the expression of miR164 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR164

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
Osa-pCAMBIA-1301-STTM167

Plasmid

#84198

Purpose

Disturb the expression of miR167 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR167

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
Osa-pCAMBIA-1301-STTM169

Plasmid

#84199

Purpose

Disturb the expression of miR169 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR169

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM171

Plasmid

#84200

Purpose

Disturb the expression of miR171 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR171

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM395

Plasmid

#84205

Purpose

Disturb the expression of miR395 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR395

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM398

Plasmid

#84208

Purpose

Disturb the expression of miR398 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR398

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
Osa-pCAMBIA-1301-STTM399

Plasmid

#84216

Purpose

Disturb the expression of miR399 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR399

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM408-5p

Plasmid

#84218

Purpose

Disturb the expression of miR408-5p in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR408-5p

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM439

Plasmid

#84222

Purpose

Disturb the expression of miR439 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR439

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM444

Plasmid

#84223

Purpose

Disturb the expression of miR444 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR444

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM528

Plasmid

#84224

Purpose

Disturb the expression of miR528 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR528

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM529

Plasmid

#84225

Purpose

Disturb the expression of miR529 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR529

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
Osa-pCAMBIA-1301-STTM530-3p

Plasmid

#84226

Purpose

Disturb the expression of miR530-3p in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR530-3p

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Gma-pCAMBIA-1301-STTM396

Plasmid

#84081

Purpose

Disturb the expression of miR396 in soybean

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR396

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Gma-pCAMBIA-1301-STTM408

Plasmid

#84082

Purpose

Disturb the expression of miR408 in soybean

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR408

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only
Osa-pCAMBIA-1301-STTM397

Plasmid

#84207

Purpose

Disturb the expression of miR397 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR397

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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